cDNA Clone ATG2B

• Plasmid: pQCXIP HA ATG2B
• Parent Plasmid: pQCXIP HA
• Expressed: HA ATG2B
• Genbank: NM_018036.6
• Tag: HA
• Species: Human

Sequence of Insert

GTCGACATGCCTTGGCCGTTTTCGGAGTCCATCAAGAAGAGGGCCTGCCG
GTACCTCCTGCAGAGGTACCTGGGCCACTTTCTGCAGGAGAAGCTGAGCC
TGGAGCAGCTCAGCCTGGACCTGTACCAAGGCACCGGGTCCCTCGCCCAG
GTCCCCTTGGACAAATGGTGTCTCAATGAGATCTTGGAGTCAGCAGATGC
ACCCTTAGAAGTCACTGAAGGATTCATTCAGTCAATTTCCCTGTCAGTTC
CATGGGGCTCTTTACTGCAGGATAATTGTGCACTGGAAGTGAGAGGATTA
GAAATGGTCTTCCGGCCTAGACCTCGCCCAGCAACTGGTTCTGAGCCTAT
GTATTGGTCAAGTTTTATGACCAGCAGTATGCAATTGGCAAAAGAATGTC
TTAGCCAGAAACTAACAGATGAACAAGGAGAAGGATCCCAGCCTTTTGAA
GGACTTGAAAAGTTTGCTGAAACCATTGAAACAGTACTAAGAAGAGTAAA
AGTCACTTTTATAGATACTGTTTTGAGAATTGAACATGTGCCAGAAAATT
CCAAAACTGGAACTGCACTTGAAATTCGAATAGAAAGAACTGTGTACTGT
GATGAAACTGCTGACGAATCCTCAGGAATTAATGTGCATCAACCCACTGC
TTTTGCTCACAAGTTACTTCAGCTCTCTGGAGTGTCTCTCTTCTGGGATG
AGTTTTCTGCATCAGCCAAATCTTCCCCAGTGTGTTCAACTGCACCAGTG
GAAACTGAGCCAAAGCTCTCACCTAGCTGGAACCCCAAAATTATTTATGA
GCCACACCCACAGCTAACTAGAAATTTACCAGAGATAGCACCTTCTGACC
CAGTGCAGATTGGACGGTTAATTGGTAGGTTGGAGTTGAGTCTCACGTTG
AAACAGAATGAAGTGCTTCCTGGAGCTAAGTTGGATGTTGATGGACAGAT
AGACTCTATTCATCTACTCCTGTCACCAAGACAGGTGCACTTGCTTTTGG
ATATGTTGGCAGCTATTGCTGGACCAGAAAATTCTAGCAAAATAGGGTTA
GCTAATAAAGATAGGAAAAATCGACCCATGCAGCAGGAAGACGAGTATCG
AATTCAGATGGAATTAAACCGGTATTATTTGAGAAAAGATTCCCTCTCTG
TGGGTGTATCTTCAGAGCAAAGCTTTTATGAGACAGAAACAGCTCGTACA
CCTTCTAGCCGTGAAGAAGAAGTATTCTTCTCCATGGCTGATATGGACAT
GTCTCATAGTCTCTCTTCTCTTCCACCCCTTGGGGACCCCCCAAACATGG
ACCTTGAGTTATCATTAACTAGTACATATACAAATACCCCAGCAGGATCT
CCATTAAGTGCTACTGTGCTTCAGCCCACTTGGGGAGAGTTCCTTGATCA
TCATAAAGAACAGCCAGTAAGAGGGTCAACATTTCCATCCAACCTAGTTC
ACCCAACACCTTTACAGAAGACATCTCTCCCATCTAGATCTGTTTCAGTG
GATGAATCAAGGCCTGAACTTATTTTTAGACTAGCTGTGGGAACTTTTTC
AATCTCTGTGCTTCACATTGATCCTTTATCTCCACCTGAAACGTCACAGA
ACCTTAATCCATTGACACCTATGGCAGTAGCTTTCTTTACTTGTATAGAA
AAGATTGATCCAGCAAGATTTTCAACAGAAGATTTTAAGTCTTTCCGAGC
AGTGTTTGCAGAAGCTTGCTCACATGATCACCTTAGATTTATAGGTACTG
GCATTAAAGTATCCTATGAACAAAGACAAAGATCAGCTTCCCGATATTTT
AGTACTGATATGTCCATTGGGCAAATGGAATTTTTGGAGTGCCTATTTCC
AACTGATTTTCATTCTGTTCCTCCTCACTATACAGAGCTTTTAACGTTCC
ATTCCAAAGAAGAAACTGGTTCCCATTCCCCTGTGTGTCTTCAGCTTCAT
TATAAGCATTCTGAGAATAGAGGGCCCCAGGGTAATCAAGCAAGACTTAG
TTCAGTTCCTCACAAGGCAGAATTGCAAATTAAATTAAATCCAGTGTGTT
GTGAGCTGGATATCAGTATTGTGGACAGGTTAAATTCCTTGCTTCAACCA
CAGAAACTTGCCACAGTAGAGATGATGGCATCCCACATGTATACTTCATA
TAATAAACATATTAGTCTGCACAAGGCTTTCACTGAAGTGTTTCTAGATG
ATTCACATAGTCCTGCAAATTGTCGGATATCAGTACAAGTTGCCACACCA
GCATTAAACCTTTCTGTTCGCTTCCCAATACCTGATCTTCGATCTGATCA
AGAAAGAGGACCATGGTTTAAGAAGTCACTTCAGAAGGAGATCCTTTATT
TAGCCTTCACAGATCTAGAATTTAAGACTGAATTTATAGGAGGATCAACC
CCAGAACAAATTAAATTGGAACTTACCTTTAGAGAACTAATTGGATCGTT
CCAGGAAGAGAAAGGAGATCCATCTATTAAGTTTTTCCATGTGTCTAGTG
GAGTAGATGGAGATACAACATCGTCAGATGACTTTGACTGGCCACGAATT
GTACTGAAAATAAATCCACCAGCCATGCATTCCATTTTGGAGAGAATTGC
AGCTGAAGAAGAAGAGGAGAATGATGGTCACTACCAGGAGGAAGAGGAAG
GAGGTGCTCATTCCTTGAAAGATGTTTGTGATCTAAGAAGACCAGCCCCA
TCTCCTTTTTCTTCTCGTAGAGTAATGTTTGAAAATGAACAGATGGTGAT
GCCAGGAGACCCTGTAGAAATGACAGAATTTCAGGATAAAGCAATCAGCA
ATTCTCACTATGTGCTGGAACTTACGTTACCAAATATTTATGTAACACTA
CCTAATAAGAGCTTTTATGAGAAGCTTTATAATAGGATCTTTAATGACTT
GCTACTGTGGGAACCAACAGCTCCTTCACCAGTGGAGACATTCGAGAATA
TTTCCTATGGCATTGGGCTTTCAGTAGCCAGTCAGCTCATTAATACTTTC
AACAAAGATAGTTTTAGTGCATTTAAATCTGCAGTTCACTATGATGAGGA
AAGTGGATCTGAGGAGGAGACTTTGCAGTATTTTTCCACTGTTGATCCCA
ACTATCGTTCTCGCAGGAAAAAAAAATTAGACTCTCAGAACAAGAACTCT
CAGAGTTTTCTCTCAGTTCTTCTGAATATTAATCATGGATTAATAGCAGT
GTTCACAGATGTGAAGCAAGATAATGGAGATCTGTTGGAAAACAAGCATG
GTGAATTCTGGTTAGAGTTCAATAGTGGTTCATTATTTTGTGTGACAAAA
TATGAAGGTTTTGATGACAAGCACTACATTTGCCTTCATTCTAGCAGTTT
CAGTCTGTACCATAAAGGCATAGTGGATGGAGTGATTCTCCCGACAGAAA
CACGACTTCCCAGCTCAACCCGCCCACACTGGTTGGAACCTACTATTTAT
TCCTCTGAAGAAGATGGCCTCAGTAAAACTTCTTCAGATGGAGTTGGAGG
AGACAGTTTGAATATGCTGTCTGTTGCCGTTAAAATATTGTCTGATAAAT
CAGAGTCCAATACAAAGGAATTTCTCATTGCCGTAGGACTGAAAGGAGCC
ACTCTCCAGCATAGAATGCTTCCTTCTGGGCTTAGCTGGCATGAGCAGAT
TTTATACTTCTTGAATATTGCTGATGAACCTGTTTTGGGATATAATCCTC
CAACTTCATTTACAACTTTTCATGTTCATCTTTGGAGCTGTGCACTTGAT
TATAGACCCCTTTATTTGCCAATCCGATCTCTTCTTACCGTGGAAACATT
CAGTGTTTCCAGTAGCGTTGCATTGGATAAATCTTCCTCTACTCTCAGAA
TAATCTTGGATGAAGCTGCTTTACATCTATCTGACAAATGCAATACTGTC
ACTATAAATCTGAGTAGAGATTATGTTCGTGTGATGGATATGGGGCTTTT
GGAGTTAACCATAACTGCAGTGAAGTCTGATTCTGATGGAGAGCAAACTG
AGCCCCGCTTTGAGTTACACTGTTCCAGCGATGTTGTCCATATCAGAACG
TGCTCAGACTCTTGTGCTGCGTTAATGAATCTCATTCAGTACATTGCAAG
CTATGGTGACTTGCAGACACCTAACAAGGCAGATATGAAGCCTGGAGCCT
TTCAAAGAAGGTCTAAGGTAGATTCCAGTGGTCGATCATCCTCACGTGGT
CCAGTACTTCCTGAAGCAGATCAACAAATGTTACGAGATCTGATGAGTGA
TGCTATGGAGGAGATCGACATGCAACAAGGCACCTCGTCAGTAAAACCAC
AGGCTAATGGTGTTTTGGATGAAAAATCTCAAATTCAGGAGCCATGTTGT
TCAGACCTCTTCCTGTTTCCTGACGAGAGTGGGAATGTATCCCAGGAGTC
CGGCCCCACCTATGCCTCATTCTCTCACCATTTCATCAGTGATGCAATGA
CAGGTGTGCCCACTGAGAATGATGACTTTTGCATTCTTTTTGCACCAAAA
GCAGCCATGCAGGAGAAGGAAGAAGAACCAGTTATAAAAATCATGGTTGA
TGATGCAATTGTGATAAGAGACAATTATTTCAGTCTGCCCGTTAATAAGA
CCGATACGAGCAAAGCCCCCTTACACTTTCCCATTCCTGTGATTCGCTAT
GTGGTGAAGGAGGTCTCTCTTGTCTGGCATCTTTATGGAGGAAAGGATTT
TGGAACAGTCCCTCCCACTTCTCCGGCTAAAAGTTATATTAGTCCCCACA
GTTCGCCTTCTCACACACCCACGAGACATGGACGTAATACAGTATGTGGG
GGAAAAGGAAGGAACCATGACTTTTTAATGGAAATACAGCTAAGCAAGGT
GAAGTTTCAGCATGAAGTCTACCCGCCATGCAAACCTGATTGTGATTCCA
GCCTCTCAGAACACCCAGTCTCCCGGCAGGTGTTCATTGTTCAGGATCTT
GAGATTCGAGATCGTTTGGCAACATCACAAATGAATAAATTTTTATACCT
GTATTGCAGTAAAGAAATGCCTCGAAAAGCTCACTCCAACATGTTGACAG
TGAAAGCCTTACACGTGTGTCCAGAATCTGGCAGGTCCCCACAGGAGTGC
TGCTTGAGAGTGTCGCTGATGCCGCTCCGCCTCAATATTGACCAGGATGC
TTTGTTCTTCCTGAAGGATTTCTTCACAAGTCTTTCTGCAGAAGTAGAGC
TTCAAATGACTCCAGATCCAGAAGTTAAAAAGTCTCCTGGAGCTGATGTC
ACCTGCAGTTTGCCAAGGCATTTGAGTACCTCAAAGGAGCCAAATCTGGT
TATTTCTTTCTCTGGGCCAAAACAGCCTTCCCAAAATGATAGTGCCAATT
CAGTGGAAGTGGTTAATGGCATGGAAGAGAAGAACTTCTCTGCTGAAGAA
GCATCTTTTAGGGATCAGCCTGTGTTTTTTAGAGAATTTAGATTCACGTC
AGAAGTTCCCATTCGACTTGATTATCATGGCAAACATGTATCAATGGATC
AGGGTACGCTAGCTGGGATTTTGATTGGTCTGGCTCAGTTAAACTGCTCT
GAACTAAAGCTCAAGAGGCTTTCCTATCGACATGGTTTACTAGGCGTTGA
CAAATTATTCTCATATGCAATCACTGAGTGGCTTAATGACATTAAGAAGA
ACCAGCTACCAGGAATCCTGGGAGGTGTTGGACCTATGCATTCACTAGTA
CAATTAGTACAAGGCCTAAAGGACTTGGTCTGGCTCCCAATAGAGCAGTA
CCGGAAGGATGGCCGCATTGTCAGAGGGTTTCAGAGAGGCGCTGCTTCCT
TTGGTACCTCGACAGCGATGGCTGCTCTAGAACTCACAAACAGAATGGTT
CAAACCATACAGGCAGCTGCAGAGACTGCTTATGATATGGTGTCTCCTGG
TACCCTTTCTATCGAGCCCAAGAAGACCAAAAGGTTTCCTCATCACCGGT
TAGCCCACCAGCCAGTAGACCTGAGGGAAGGTGTGGCCAAGGCCTACAGT
GTTGTGAAAGAGGGAATCACAGACACGGCTCAGACCATTTATGAAACTGC
GGCTCGAGAACACGAGAGCAGAGGGGTGACTGGTGCCGTGGGCGAGGTTC
TGCGCCAGATTCCTCCGGCAGTGGTGAAACCTCTGATTGTTGCCACAGAA
GCAACGTCAAACGTGCTGGGTGGCATGAGAAACCAAATTAGGCCAGATGT
CCGGCAAGACGAGTCACAGAAATGGCGCCACGGGGATGACTGAGCGGCCG
C

Amino Acid Sequence

MYPYDVPDYAGSPGEFASLDMPWPFSESIKKRACRYLLQRYLGHFLQEKL
SLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLS
VPWGSLLQDNCALEVRGLEMVFRPRPRPATGSEPMYWSSFMTSSMQLAKE
CLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPE
NSKTGTALEIRIERTVYCDETADESSGINVHQPTAFAHKLLQLSGVSLFW
DEFSASAKSSPVCSTAPVETEPKLSPSWNPKIIYEPHPQLTRNLPEIAPS
DPVQIGRLIGRLELSLTLKQNEVLPGAKLDVDGQIDSIHLLLSPRQVHLL
LDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSL
SVGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPN
MDLELSLTSTYTNTPAGSPLSATVLQPTWGEFLDHHKEQPVRGSTFPSNL
VHPTPLQKTSLPSRSVSVDESRPELIFRLAVGTFSISVLHIDPLSPPETS
QNLNPLTPMAVAFFTCIEKIDPARFSTEDFKSFRAVFAEACSHDHLRFIG
TGIKVSYEQRQRSASRYFSTDMSIGQMEFLECLFPTDFHSVPPHYTELLT
FHSKEETGSHSPVCLQLHYKHSENRGPQGNQARLSSVPHKAELQIKLNPV
CCELDISIVDRLNSLLQPQKLATVEMMASHMYTSYNKHISLHKAFTEVFL
DDSHSPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEIL
YLAFTDLEFKTEFIGGSTPEQIKLELTFRELIGSFQEEKGDPSIKFFHVS
SGVDGDTTSSDDFDWPRIVLKINPPAMHSILERIAAEEEEENDGHYQEEE
EGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAI
SNSHYVLELTLPNIYVTLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFE
NISYGIGLSVASQLINTFNKDSFSAFKSAVHYDEESGSEEETLQYFSTVD
PNYRSRRKKKLDSQNKNSQSFLSVLLNINHGLIAVFTDVKQDNGDLLENK
HGEFWLEFNSGSLFCVTKYEGFDDKHYICLHSSSFSLYHKGIVDGVILPT
ETRLPSSTRPHWLEPTIYSSEEDGLSKTSSDGVGGDSLNMLSVAVKILSD
KSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYN
PPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTL
RIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAVKSDSDGEQ
TEPRFELHCSSDVVHIRTCSDSCAALMNLIQYIASYGDLQTPNKADMKPG
AFQRRSKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEIDMQQGTSSVK
PQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQESGPTYASFSHHFISDA
MTGVPTENDDFCILFAPKAAMQEKEEEPVIKIMVDDAIVIRDNYFSLPVN
KTDTSKAPLHFPIPVIRYVVKEVSLVWHLYGGKDFGTVPPTSPAKSYISP
HSSPSHTPTRHGRNTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPDCD
SSLSEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNML
TVKALHVCPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEV
ELQMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVISFSGPKQPSQNDSA
NSVEVVNGMEEKNFSAEEASFRDQPVFFREFRFTSEVPIRLDYHGKHVSM
DQGTLAGILIGLAQLNCSELKLKRLSYRHGLLGVDKLFSYAITEWLNDIK
KNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAA
SFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKTKRFPHH
RLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGE
VLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29881
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent change C1719T, contains listed SNP N1124D; Parental 59719
• Low Temp: No