cDNA Clone ARI-1 (C. elegans)

• Plasmid: pET15b 6HIS TEV ARI-1 C.elegans
• Parent Plasmid: pET15b 6His TEV
• Expressed: 6HIS TEV ARI-1 C.elegans
• Genbank: CCD61206.1
• Tag: 6His
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGAGTTCCGACGATGAGATCAATATGGACGACAGTGACTCAAGTCAAGGAGAAATTGACGATGGGTGTATGTCTGACGATGATGGCATCGTACTGGAGAGCCGCGAACAGAATAGCTCCGACTACAAAGATAACGGGGAACCGGATAATGAGGTGCTAAACCACGATTCCTTGGAAGCAGAGATGAAAAAGACAATAACAGATGTACAAGCAGTTCTTCAAGTGAAAACTGGTGTCTGTCGGATTCTTCTGCACAAGTATAAATGGAATAAGGAATCATTATTAGAACGATTCTACGAACATCCTGACACTACCACATTTCTTATCGATGCTCATGTTATTCCACGTCGCCAGGAGCGTTTGCCGGCTGGAGACGCGGAATGCGACATATGTTGCTCGTTGGGAGAACTCTCTGGGCTTTCTTGTAATCACAGAGCTTGTACACAATGCTGGAAGGCGTATTTAACTAATAAGATTGCAAACAATGCACAATCCGAAATAGAATGTATGGCTCCAAATTGCAAATTGCTCATAGAAGATGAAAAGGTTATGTTCTACATTACCGACCCAACAGTAATTGCCACCTACAGAAAGCTGATTGTGGCTAGTTATGTGGAAACGAATCGTCTTCTCAAGTGGTGCCCCGGTATAGATTGTGGGAAAGCTGTTAGAGTCAGCCATTGGGAACCCCGCCTCGTCGTATGCTCCTGTGGATCTCGATTCTGCTTCTCTTGTGGTCACGATTGGCATGAACCAGTGAACTGTAGGCTGCTGAAGTTATGGCTTAAAAAGTGCAATGATGATAGTGAAACATCGAATTGGATTAATGCAAACACTAAAGAATGTCCAAAATGTATGATCACAATTGAAAAAGACGGTGGATGCAATCATATGACGTGTAAAAATACAGCATGCCGGTTCGAATTTTGTTGGATGTGCCTTGGTCCATGGGAACCTCATGGTTCATCATGGTATAGTTGTAATAGATTTGATGATTCTGCAGCTAAAAATGCTCGGGATGCTCAAGAAGTATCACGTGCAAATCTTCAAAGATATCTTTTCTATTATAATCGATATATGGGACATCAACAAAGTTTACGTTTGGAGGGAAAGCTCTATGCAACCGTCAAATCAAAAATGGAGCAAATGCAGACACTTTCCATGTCTTGGATTGAAGTTCAATTCCTTCGGAAAGCTGTCGATGTATTGTCAGAATGCCGTCGGACACTGATGTTCACTTATGCATTCGCATTTTATTTGAAGCGAGATAATAACGCCATTATTTTTGAATCAAATCAAAAAGATCTCGAAATGGAAACAGAACAGCTGTCCGGGTTTTTGGAACGAGATTTAGACAATGAGAATCTGGTGACACTTAAACAAAAGGTCCAAGACAAGTACCGATACGTAGAACATCGCCGAAAAGTACTTCTAGACCATTGTTCCGAAGGAGCTGATCAAGAACTTTGGGTTTTCAACGAGTAAGCGGCCGCG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGGSMSSDDEINMDDSDSSQGEIDDGCMSDDDGIVLESREQNSSDYKDNGEPDNEVLNHDSLEAEMKKTITDVQAVLQVKTGVCRILLHKYKWNKESLLERFYEHPDTTTFLIDAHVIPRRQERLPAGDAECDICCSLGELSGLSCNHRACTQCWKAYLTNKIANNAQSEIECMAPNCKLLIEDEKVMFYITDPTVIATYRKLIVASYVETNRLLKWCPGIDCGKAVRVSHWEPRLVVCSCGSRFCFSCGHDWHEPVNCRLLKLWLKKCNDDSETSNWINANTKECPKCMITIEKDGGCNHMTCKNTACRFEFCWMCLGPWEPHGSSWYSCNRFDDSAAKNARDAQEVSRANLQRYLFYYNRYMGHQQSLRLEGKLYATVKSKMEQMQTLSMSWIEVQFLRKAVDVLSECRRTLMFTYAFAFYLKRDNNAIIFESNQKDLEMETEQLSGFLERDLDNENLVTLKQKVQDKYRYVEHRRKVLLDHCSEGADQELWVFNE*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15b 6HIS TEV ARI-1 C.elegans60.9 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU62230
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 6HIS TEV same as in pFBHTb (vector DU62186)
• Low Temp: No