cDNA Clone CK1α

• Synonyms: Casein kinase I isoform alpha
• Plasmid: pBabeD puro cVenus-5Gly-FLAG A206K CK1 alpha
• Parent Plasmid: pBabeD puro
• Expressed: cVenus-5Gly-FLAG A206K CK1 alpha
• Genbank: P48729.2
• Species: Human

Sequence of Insert

AGATCTATGGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCA
CAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAACA
CCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGC
TACCAGTCCAAACTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGT
CCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGC
TGTACAAGGGTGGCGGAGGTGGAATGGACTACAAGGACGATGACGATAAG
GGATCCATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTCGGAGG
GAAATATAAACTGGTACGGAAGATCGGGTCTGGCTCCTTCGGGGACATCT
ATTTGGCGATCAACATCACCAACGGCGAGGAAGTGGCAGTGAAGCTAGAA
TCTCAGAAGGCCAGGCATCCCCAGTTGCTGTACGAGAGCAAGCTCTATAA
GATTCTTCAAGGTGGGGTTGGCATCCCCCACATACGGTGGTATGGTCAGG
AAAAAGACTACAATGTACTAGTCATGGATCTTCTGGGACCTAGCCTCGAA
GACCTCTTCAATTTCTGTTCAAGAAGGTTCACAATGAAAACTGTACTTAT
GTTAGCTGACCAGATGATCAGTAGAATTGAATATGTGCATACAAAGAATT
TTATACACAGAGACATTAAACCAGATAACTTCCTAATGGGTATTGGGCGT
CACTGTAATAAGTTATTCCTTATTGATTTTGGTTTGGCCAAAAAGTACAG
AGACAACAGGACAAGGCAACACATACCATACAGAGAAGATAAAAACCTCA
CTGGCACTGCCCGATATGCTAGCATCAATGCACATCTTGGTATTGAGCAG
AGTCGCCGAGATGACATGGAATCATTAGGATATGTTTTGATGTATTTTAA
TAGAACCAGCCTGCCATGGCAAGGGCTAAAGGCTGCAACAAAGAAACAAA
AATATGAAAAGATTAGTGAAAAGAAGATGTCCACGCCTGTTGAAGTTTTA
TGTAAGGGGTTTCCTGCAGAATTTGCGATGTACTTAAACTATTGTCGTGG
GCTACGCTTTGAGGAAGCCCCAGATTACATGTATCTGAGGCAGCTATTCC
GCATTCTTTTCAGGACCCTGAACCATCAATATGACTACACATTTGATTGG
ACAATGTTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCA
GGGTCAGCAGGCCCAAACCCCCACAGGCAAGCAAACTGACAAAACCAAGA
GTAACATGAAAGGTTTCTAAGCGGCCGC

Amino Acid Sequence

MDKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSY
QSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGGGGMDYKDDDDKG
SMASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLES
QKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLED
LFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRH
CNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQS
RRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLC
KGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWT
MLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29022
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Tyr17 and Thr330 restored (NCBI update); Parental 33769.
• Low Temp: No