cDNA Clone CK1ε

• Plasmid: pcDNA5-FRT/TO-mCherry-CK1ε N133A
• Parent Plasmid: pcDNA5-FRT/TO-mCherry
• Expressed: mcherry-CK1ε N133A
• Genbank: NM_152221
• Tag: mCherry

Sequence of Insert

agatctATGGAGCTACGTGTGGGGAACAAGTACCGCCTGGGACGGAAGAT
CGGGAGCGGGTCCTTCGGAGATATCTACCTGGGTGCCAACATCGCCTCTG
GTGAGGAAGTCGCCATCAAGCTGGAGTGTGTGAAGACAAAGCACCCCCAG
CTGCACATCGAGAGCAAGTTCTACAAGATGATGCAGGGTGGCGTGGGGAT
CCCGTCCATCAAGTGGTGCGGAGCTGAGGGCGACTACAACGTGATGGTCA
TGGAGCTGCTGGGGCCTAGCCTCGAGGACCTGTTCAACTTCTGTTCCCGC
AAATTCAGCCTCAAGACGGTGCTGCTCTTGGCCGACCAGATGATCAGCCG
CATCGAGTATATCCACTCCAAGAACTTCATCCACCGGGACGTCAAGCCCG
ACgcCTTCCTCATGGGGCTGGGGAAGAAGGGCAACCTGGTCTACATCATC
GACTTCGGCCTGGCCAAGAAGTACCGGGACGCCCGCACCCACCAGCACAT
TCCCTACCGGGAAAACAAGAACCTGACCGGCACGGCCCGCTACGCTTCCA
TCAACACGCACCTGGGCATTGAGCAAAGCCGTCGAGATGACCTGGAGAGC
CTGGGCTACGTGCTCATGTACTTCAACCTGGGCTCCCTGCCCTGGCAGGG
GCTCAAAGCAGCCACCAAGCGCCAGAAGTATGAACGGATCAGCGAGAAGA
AGATGTCAACGCCCATCGAGGTCCTCTGCAAAGGCTATCCCTCCGAATTC
TCAACATACCTCAACTTCTGCCGCTCCCTGCGGTTTGACGACAAGCCCGA
CTACTCTTACCTACGTCAGCTCTTCCGCAACCTCTTCCACCGGCAGGGCT
TCTCCTATGACTACGTCTTTGACTGGAACATGCTGAAATTCGGTGCAGCC
CGGAATCCCGAGGATGTGGACCGGGAGCGGCGAGAACACGAACGCGAGGA
GAGGATGGGGCAGCTACGGGGGTCCGCGACCCGAGCCCTGCCCCCTGGCC
CACCCACGGGGGCCACTGCCAACCGGCTCCGCAGTGCCGCCGAGCCCGTG
GCTTCCACGCCAGCCTCCCGCATCCAGCCGGCTGGCAATACTTCTCCCAG
AGCGATCTCGCGGGTCGACCGGGAGAGGAAGGTGAGTATGAGGCTGCACA
GGGGTGCGCCCGCCAACGTCTCCTCCTCAGACCTCACTGGGCGGCAAGAG
GTCTCCCGGATCCCAGCCTCACAGACAAGTGTGCCATTTGACCATCTCGG
GAAGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLGSMELRVGNKY
RLGRKIGSGSFGDIYLGANIASGEEVAIKLECVKTKHPQLHIESKFYKMM
QGGVGIPSIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLA
DQMISRIEYIHSKNFIHRDVKPDAFLMGLGKKGNLVYIIDFGLAKKYRDA
RTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLG
SLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFSTYLNFCRSLR
FDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDVDRERR
EHEREERMGQLRGSATRALPPGPPTGATANRLRSAAEPVASTPASRIQPA
GNTSPRAISRVDRERKVSMRLHRGAPANVSSSDLTGRQEVSRIPASQTSV
PFDHLGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28840
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 31558
• Low Temp: No