cDNA Clone CDKL5 (mouse)

• Synonyms: BC038161; Stk9
• Plasmid: pBabeD puro GFP mouse CDKL5 T169E Y171E
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP-mouse CDKL5 T169E Y171E
• Genbank: NM_001024624.2 GI:167004177
• Tag: GFP
• Species: Mouse

Sequence of Insert

GGATCTGCCACCATGCATGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCA
GCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGT
GACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAG
GAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCA
TCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCAT
CAAGGTCCCGCTCTCGGCATGGAGAGCTGTACAGTCCGGACTCGGATCCATGAAGATTCCTAACATTGGTAATGTGATGAATAAATTTGAGATCCTTGGG
GTTGTCGGTGAAGGAGCCTATGGAGTTGTACTTAAATGCAGACACAAGGAAACACATGAAATTGTGGCAATCAAGAAATTCAAGGACAGCGAAGAAAATG
AAGAAGTCAAGGAGACGACCTTACGAGAGCTTAAAATGCTTCGCACTCTCAAGCAGGAAAACATTGTGGAGCTGAAGGAAGCTTTTCGTCGGAGAGGAAA
ATTATACCTGGTGTTTGAATATGTTGAGAAGAATATGCTTGAATTGCTGGAAGAAATGCCAAATGGAGTTCCACCTGAAAAAGTCAAGAGCTATATCTAC
CAACTAATCAAAGCTATCCACTGGTGCCACAAGAACGACATCGTCCACAGAGATATAAAGCCTGAAAATCTCTTAATCAGCCACAATGATGTCCTGAAAC
TATGTGACTTTGGTTTTGCTCGCAATCTCTCAGAGGGCAATAATGCTAATTATGAGGAGGAGGTGGCTACAAGGTGGTACCGATCCCCAGAACTGCTACT
TGGAGCCCCCTATGGAAAGTCAGTGGACATGTGGTCAGTGGGCTGTATTCTTGGAGAGCTTAGTGATGGCCAGCCCTTATTTCCTGGAGAAAGTGAAATT
GACCAACTCTTTACTATTCAGAAGGTGCTAGGACCACTTCCATCTGAGCAAATGAAGCTCTTCTACAGTAACCCTCGTTTCCATGGGCTCCGGTTCCCAG
CTGTTAACCATCCTCAGTCATTAGAGAGAAGATACCTTGGAATTTTAAATAGTGTTTTACTTGACCTAATGAAGAATTTACTGAAGTTGGACCCAGCTGA
CAGATACTTGACAGAGCAGTGTTTAAATCATCCTACATTTCAGACTCAGAGACTTTTGGACCGGTCTCCTTCAAGATCAACAAAAAGGAAACCTTATCAT
GTGGAAAGCAGCACCCTGTCTAATAGAAACCAATCCACCAAGGGTGCTGCCTTACAGACTCACCACAGATCCAACAGCAAGGACATTCAGAACTTAAGTG
TGGGTCTGCCCAGGGCTGAGGAAGGCCTGCCTGCAAACGAAAGCTTCCTCAATGGAAACCTTGCTGGAGCAACTCTCAGCCCAATGCACACCAAAACCTA
CCAAGCAAGCACCCAGCCTGGCTCTTCCAGCAAAGATCTCACAAACAACAACATACCACACCTTCTCAGTCCAAAAGAAGCCAAGTCCAAAACAGAGTTT
GACTTCAATATTGACACAAAGCCTTCAGAAGGTCCAGGCACAAAGTACCTCAAGTCCAGCACCAGATCTCAGCAGAACAGGCACTCATTCATGGAAAGCT
CCCAGAGCAAAGCTGGGACCCTGCAGCCCAGTGAGAAGCAGAGTCGGCACAGCTATATTGACACCATTCCCCAGTCCTCCCGGAGTCCATCCTACAGGAC
AAAGGCCAAAAGCCATGGGGCGTTGAGTGACTCCAAGTCTGTGAGCAACCTTTCCGAAGCCAGAGCCCAAATCACAGAGACTAATACCAGTAGATACTTC
CCATCCAGTTGTTTGGACTTGAACTCTCCCACCAGCCCAACCCCCACCAGGCACACTGATACGAGAACTTTGCTCAGCCCTTCCGGTCGAAATAATAGAA
ATGAAGGCACTCTGGACTCACGGCGAACCACCACTAGACACTCAAAAACCATGGAGGAGTTGAAGCTGCCTGAACACATGGACAGCAGCCATTCGCATTC
ACTGTCTGCACCTCATGAATCATTTTCTTATGGGCTAGGCTACACCAGCCCCTTCTCCTCTCAGCAGCGTCCTCACAGGCATTCCATGTACGTGACCCGG
GACAAAGTGAGAGCGAAAGGCCTTGATGGAAGCTTGAGCATAGGACAAGGGATGGCAGCCAGAGCCAACAGTTTGCAGCTCTTATCACCCCAGCCTGGAG
AACAGCTCCCTCCAGAGATGACTGTGGCAAGACCTTCTGTTAAAGAGTCCTCCCGAGAAGGCGCCTCTTCATTCCACACCCGTCAGAAGTCTGAGGGTGG
AGTATACCATGACCCACACTCTGACGACGGCACAGCTCCCAAAGAAAATCGGCATCTGTACAATGACCCTGTCCCAAGGAGAGTTGGTAGCTTTTATAGA
GTGCCATCTCCACGACCAGACAATTCTTTCCATGAAAATAATGTGTCCACTCGAGTTTCTTCTCTGCCATCTGATAGCAGTTCTGGTACAAATCATTCAA
AAAGACAACCAGGATTTGATCCATGGAAAAGTCCTGAAAACATTAGTCATGCCGATCAACTCAAAGAAAAGGAGAAGCAAGGTTTTTTCAGGTCAATGAA
AAAGAAAAAGAAGAAAACTCAAACAGTGCCCAACACTGACGGTCCTGATCTATTGACCTTACAGAAAGCCATTCATTCTTCTAGCACTGCAAGCAGCAGA
CCAAAGGAGTGGCGACCTGAGAAGCTCTCAGATCTACAGACCCAGAGCCAACCACTAAAATCTCTTCGAAAGCTGTTGCATCTGTCTTCATCAACCAATC
ATCCAGCTTCTTCAGATCCTCGGTTCCAGCCCTTAACAGCTCAACAAGCCAAAAATTCCTTCTCAGAAATTCGGATTCACCCTCTGAGTCAGGCCACTGG
TGGGAGCAGCAACATCCGGCAGGAGCCTACACCAAAAGGCAGACCAGCCCTTCAGTTGCCAGGATCTTCCCTCTTAAGGTACAACGGATGGAAACACTCA
AGATCAAGATCCAGCCAGCCAGACGAAGTGATCTTCTTGGCATCCCATGAGAAATGGAAACAGTGAGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSATMHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTG
KLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVY
IMADKQKNGIKVPLSAWRAVQSGLGSMKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQENIVE
LKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGNNANYEEEVAT
RWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSVLLDLM
KNLLKLDPADRYLTEQCLNHPTFQTQRLLDRSPSRSTKRKPYHVESSTLSNRNQSTKGAALQTHHRSNSKDIQNLSVGLPRAEEGLPANESFLNGNLAGA
TLSPMHTKTYQASTQPGSSSKDLTNNNIPHLLSPKEAKSKTEFDFNIDTKPSEGPGTKYLKSSTRSQQNRHSFMESSQSKAGTLQPSEKQSRHSYIDTIP
QSSRSPSYRTKAKSHGALSDSKSVSNLSEARAQITETNTSRYFPSSCLDLNSPTSPTPTRHTDTRTLLSPSGRNNRNEGTLDSRRTTTRHSKTMEELKLP
EHMDSSHSHSLSAPHESFSYGLGYTSPFSSQQRPHRHSMYVTRDKVRAKGLDGSLSIGQGMAARANSLQLLSPQPGEQLPPEMTVARPSVKESSREGASS
FHTRQKSEGGVYHDPHSDDGTAPKENRHLYNDPVPRRVGSFYRVPSPRPDNSFHENNVSTRVSSLPSDSSSGTNHSKRQPGFDPWKSPENISHADQLKEK
EKQGFFRSMKKKKKKTQTVPNTDGPDLLTLQKAIHSSSTASSRPKEWRPEKLSDLQTQSQPLKSLRKLLHLSSSTNHPASSDPRFQPLTAQQAKNSFSEI
RIHPLSQATGGSSNIRQEPTPKGRPALQLPGSSLLRYNGWKHSRSRSSQPDEVIFLASHEKWKQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU51845
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee