cDNA Clone CUL2 (mouse)

• Synonyms: cullin2
• Plasmid: pMEX3C CUL2 (mouse) 1-222
• Parent Plasmid: pMEX3C
• Expressed: MBP CUL2 (mouse) 1-222
• Genbank: NP_083678.2
• Tag: MBP
• Species: Mouse

Sequence of Insert

GGATCCATGTCTTTAAAACCAAGAGTAGTGGATTTTGATGAGACATGGAACAAACTCCTAACTACAATCAAAGCTGTTGTTATGTTGGAATATGTCGAGC
GAGCAACATGGAATGACCGCTTCTCAGATATCTATGCTTTATGTGTGGCCTATCCTGAACCACTTGGAGAAAGACTTTATGCGGAAACAAAGATTTTTTT
GGAAAGTCACGTTCGGCATTTGTATAAGAGAGTTCTGGAGTCTGAAGAACAAGTGCTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAAGGCGCC
GACTATATGGACTGCTTATATAGGTATCTCAATACCCAGTATATTAAAAAGAATAAATTGACAGAAGCTGATATACAGTATGGCTATGGTGGAGTAGATA
TGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGGAAGTTAATGGTTGAACCCCTTCAGAACATCCTCATCCGGATGCTGCTTCG
AGAAATTAAAAATGATCGTGGTGGTGAAGATCCAAACCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTT
CCCTTAAAGTTTTATCAGGGAATATTCGTGTCTCCCTTTCTGACTGAAACGGGAGAATATTACAAGCAAGAATAAGCGGCCG

Amino Acid Sequence

MMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVR
YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDL
IKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQG
PLMSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYAETKIFLESHVRHLYKRVLESEEQVLVMYHRYWEEYSKGA
DYMDCLYRYLNTQYIKKNKLTEADIQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQNILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKF
PLKFYQGIFVSPFLTETGEYYKQE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51827
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct of M. musculus Cullin2 (1-222), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.