cDNA Clone Brd4 (ms)

• Plasmid: pCAGGS 3xFLAG ms Brd4
• Parent Plasmid: pCAGGS 3xFLAG
• Expressed: FLAG-mouse Brd4
• Tag: 3FLAG
• Species: Mouse

Sequence of Insert

ggatccATGTCTACGGAGAGCGGCCCTGGGACAAGATTGAGAAATCTGCCAGTAATGGGGGATGGACTAGAAACCTCCCAAATGTCTACAACGCAGGCCCAGGCCCAACCCCAGCCAGCAAATGCAGCCAGCACCAATCCTCCACCCCCAGAGACCTCCAACCCTAACAAGCCCAAGAGACAGACAAACCAACTGCAATATCTGCTCAGAGTGGTGCTCAAGACACTATGGAAACACCAGTTTGCGTGGCCTTTCCAGCAGCCCGTGGATGCCGTCAAGCTGAACCTCCCTGATTACTATAAGATTATTAAAACACCCATGGATATGGGAACAATAAAGAAGCGCTTGGAAAACAACTATTACTGGAATGCTCAGGAATGTATCCAGGACTTCAACACTATGTTTACAAATTGTTACATCTATAACAAGCCTGGAGATGACATCGTCTTAATGGCAGAAGCTCTGGAGAAGCTCTTCTTGCAAAAAATCAATGAACTGCCTACAGAAGAAACTGAGATCATGATAGTCCAGGCAAAAGGAAGAGGACGAGGGAGGAAAGAAACAGGGGCAGCAAAGCCTGGTGTATCCACGGTACCAAACACAACTCAAGCATCAACTTCTCCGCAGACCCAGACGCCTCAGCAGAACCCTCCTCCACCTGTGCAGGCCACAACTCACCCCTTTCCTGCTGTCACCCCAGACCTCATTGCCCAGCCTCCTGTCATGACAATGGTGCCCCCTCAGCCACTTCAGACTCCTTCACCGGTACCCCCCCAGCCACCACCCCCACCTGCTCCAGTTCCACAGCCTGTGCAGAGTCACCCGCCCATCATTGCGACCACCCCCCAGCCTGTGAAGACAAAGAAAGGGGTGAAGAGGAAAGCAGATACCACCACCCCTACCACCATCGACCCCATTCATGAGCCACCCTCACTGGCCCCAGAGCCCAAGACCGCCAAGCTGGGTCCTCGGCGGGAGAGCAGCAGACCTGTGAAGCCTCCAAAGAAGGATGTACCGGACTCACAGCAGCACCCAGGGCCAGAGAAGAGCAGCAAGATCTCTGAGCAGCTAAAGTGCTGCAGTGGCATCCTCAAGGAGATGTTTGCCAAGAAACATGCTGCCTATGCCTGGCCTTTCTACAAGCCTGTGGATGTGGAGGCACTGGGTCTGCACGACTACTGTGACATCATCAAACATCCCATGGACATGAGCACAATCAAGTCTAAACTAGAGTCCCGAGAGTACAGAGATGCCCAGGAATTTGGTGCTGATGTCCGATTGATGTTCTCCAACTGCTACAAGTACAACCCCCCTGACCATGAAGTGGTAGCCATGGCTCGAAAACTCCAGGATGTGTTTGAAATGCGCTTTGCCAAGATGCCTGATGAGCCTGAAGAGCCAGTTGTTACAGTGTCCTCTCCTGCAGTGCCACCCCCTACAAAGGTGGTAGCCCCACCCTCATCTAGTGACAGCAGCAGCGACAGTTCTTCCGACAGCGACAGTTCCACTGACGACTCTGAGGAAGAGCGAGCCCAGCGGCTGGCTGAACTCCAGGAACAGCTCAAGGCCGTGCATGAGCAGCTTGCAGCCCTCTCACAGCCCCAGCAGAACAAACCAAAGAAAAAGGAGAAGGACAAGAAGGAAAAGAAAAAGGAAAAGCACAAAAAGAAAGAAGAAGTGGAGGAAAATAAAAAAAGCAAAACCAAGGAACTTCCTCCCAAAAAGACAAAGAAAAATAACAGCAGCAACAGCAATGTGAGCAAGAAGGAACCAGTACCCACGAAGACCAAGCCGCCTCCCACATATGAATCAGAAGAGGAGGATAAGTGTAAGCCCATGTCTTATGAGGAGAAGCGGCAGCTAAGTCTAGATATCAACAAACTTCCTGGTGAGAAGCTAGGCCGTGTAGTACACATAATTCAGTCAAGGGAACCATCACTTAAAAACTCCAACCCCGATGAGATTGAGATTGACTTTGAGACCCTGAAGCCATCTACACTACGAGAGTTGGAGCGATATGTCACCTCCTGTTTGCGGAAGAAAAGGAAACCTCAAGCTGAAAAAGTTGACGTGATTGCTGGTTCTTCCAAGATGAAGGGATTCTCATCCTCTGAGTCGGAGAGCACCAGCGAATCCAGCTCCTCTGACAGTGAAGACTCTGAAACAGAGATGGCTCCCAAGTCAAAAAAGAAGGGGCACACTGGGAGGGACCAGAAAAAGCATCATCACCATCACCATCCACAGATGCAGCCAGCCCCAGCTCCTGTGCCCCAGCAGCCGCCCCCACCTCCACAGCAGCCTCCTCCACCCCCACCTCCGCAGCAGCAGCAGCAGCAACCTCCACCCCCACCACCTCCGCCCTCCATGCCACAGCAGACTGCCCCAGCGATGAAGTCCTCGCCCCCACCCTTCATCACTGCCCAGGTCCCCGTCCTGGAACCACAGCTGCCAGGCAGTGTCTTTGACCCTATTGGCCACTTCACCCAGCCCATCTTGCACCTGCCGCAGCCGGAGCTGCCTCCTCACCTGCCCCAGCCACCTGAGCACAGCACTCCACCCCATCTCAACCAGCATGCTGTGGTCTCTCCTCCAGCTTTGCACAATGCGCTGCCCCAACAGCCATCTCGGCCCAGTAACCGAGCTGCTGCTCTGCCCCCAAAGCCTACCCGACCCCCAGCTGTGTCCCCTGCCCTGGCCCAGCCCCCCCTGCTCCCACAACCACCAATGGCTCAGCCCCCCCAAGTGCTGCTGGAGGATGAAGAGCCACCTGCCCCACCCCTCACCTCCATGCAGATGCAGTTGTACCTACAGCAGCTGCAGAAGGTGCAGCCTCCCACACCACTACTCCCTTCCGTGAAGGTGCAGTCCCAACCCCCACCGCCTTTGCCGCCTCCACCCCACCCTTCTGTGCAGCAGCAGCAGCTCCAGCCACAGCCACCGCCACCCCCACCTCCTCAGCCACAGCCACCACCCCAGCAACAACACCAGCCTCCACCACGACCAGTTCACTTGCCATCCATGCCCTTTTCTGCTCATATTCAGCAGCCCCCACCACCCCCAGGACAGCAACCTACTCACCCACCCCCAGGACAGCAGCCCCCACCACCACAGCCTGCCAAGCCCCAGCAAGTCATCCAGCATCACCCTTCCCCCCGGCACCACAAGTCAGACCCCTACTCAGCTGGTCATCTTCGTGAGGCTCCCTCTCCGCTTATGATACATTCCCCTCAGATGCCACAGTTCCAGAGCCTGACCCATCAGTCTCCTCCCCAGCAAAACGTCCAGCCTAAGAAGCAGGAGCTGCGACCACCTTCAGTCGTCCAGCCCCAGCCCCTGGTGGTAGTAAAGGAGGAGAAGATTCACTCACCAATCATTCGCAGCGAGCCTTTCAGCACCTCACTTCGACCAGAGCCCCCCAAGCACCCAGAGAACATCAAGGCCCCAGTCCACCTGCCCCAGCGGCCTGAGATGAAGCCTGTAGACATAGGGAGGCCTGTGATCCGGCCTCCAGAGCAGAGTGCACCCCCACCAGGGGCCCCTGACAAGGACAAACAGAAGCAGGAGCCAAAAACACCAGTGGCACCCAAAAAGGACCTGAAAATTAAGAACATGGGCTCCTGGGCCAGCCTGGTACAGAAGCATCCGACCACCCCATCCTCCACAGCCAAGTCCTCAAGTGACAGCTTTGAGCATTTCCGCCGTGCTGCTCGGGAGAAGGAGGAGAGGGAAAAGGCCCTGAAGGCTCAGGCTGAGCATGCAGAGAAGGAGAAGGAGCGGCTGAGGCAGGAGCGCATGAGGAGCCGAGAGGATGAAGATGCGCTGGAACAGGCACGTCGAGCCCATGAGGAGGCACGACGGCGCCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGAGACAGGAGCAGCAGCAGCAGCAGCAGCAGGCAGCTGCTGTGGCCGCTGCCTCTGCCCCCCAGGCCCAGAGCTCCCAGCCCCAGTCTATGCTGGACCAGCAGAGGGAGTTGGCCCGGAAACGAGAGCAGGAGCGGAGGCGCAGGGAGGCAATGGCAGCTACAATTGACATGAATTTCCAGAGTGATCTTTTGTCAATATTTGAAGAAAATCTTTTTTGAGCGGCCGC

Amino Acid Sequence

MDYKDHDGDYKDHDIDYKDDDDKGSMSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF*

• Antibiotic: Amp
• DU Number: DU55083
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Could you please clone mouse Brd4 into pCAGGs with an N-terminal FLAG tag? DU46334 might be used as template.