cDNA Clone CK1α

• Synonyms: Casein kinase I isoform alpha
• Plasmid: pSc-B-CK1 alpha
• Parent Plasmid: pSC-B
• Genbank: P48729.2
• Species: Human

Sequence of Insert

GGATCCATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTCGGAGG
GAAAAATAAACTGGTACGGAAGATCGGGTCTGGCTCCTTCGGGGACATCT
ATTTGGCGATCAACATCACCAACGGCGAGGAAGTGGCAGTGAAGCTAGAA
TCTCAGAAGGCCAGGCATCCCCAGTTGCTGTACGAGAGCAAGCTCTATAA
GATTCTTCAAGGTGGGGTTGGCATCCCCCACATACGGTGGTATGGTCAGG
AAAAAGACTACAATGTACTAGTCATGGATCTTCTGGGACCTAGCCTCGAA
GACCTCTTCAATTTCTGTTCAAGAAGGTTCACAATGAAAACTGTACTTAT
GTTAGCTGACCAGATGATCAGTAGAATTGAATATGTGCATACAAAGAATT
TTATACACAGAGACATTAAACCAGATAACTTCCTAATGGGTATTGGGCGT
CACTGTAATAAGTTATTCCTTATTGATTTTGGTTTGGCCAAAAAGTACAG
AGACAACAGGACAAGGCAACACATACCATACAGAGAAGATAAAAACCTCA
CTGGCACTGCCCGATATGCTAGCATCAATGCACATCTTGGTATTGAGCAG
AGTCGCCGAGATGACATGGAATCATTAGGATATGTTTTGATGTATTTTAA
TAGAACCAGCCTGCCATGGCAAGGGCTAAAGGCTGCAACAAAGAAACAAA
AATATGAAAAGATTAGTGAAAAGAAGATGTCCACGCCTGTTGAAGTTTTA
TGTAAGGGGTTTCCTGCAGAATTTGCGATGTACTTAAACTATTGTCGTGG
GCTACGCTTTGAGGAAGCCCCAGATTACATGTATCTGAGGCAGCTATTCC
GCATTCTTTTCAGGACCCTGAACCATCAATATGACTACACATTTGATTGG
ACAATGTTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCA
GGGTCAGCAGGCCCAAACCCCCACAGGCAAGCAAACTGACAAATCCAAGA
GTAACATGAAAGGTTTCTAAGCGGCCGC

Amino Acid Sequence

MASSSGSKAEFIVGGKNKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQ
KARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDL
FNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHC
NKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSR
RDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCK
GFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTM
LKQKAAQQAASSSGQGQQAQTPTGKQTDKSKSNMKGF*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU28167
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: clone has the following mutations compared to NCBI ref Tyr17>Asn and Thr330>Ser