cDNA Clone ARHGEF5

• Synonyms: GEF5, P60, TIM, TIM1
• Plasmid: pSCB ARHGEF5 1029-end template only
• Parent Plasmid: pSC-B
• Species: Human

Sequence of Insert

GCCAACAAGCACAAGGGCTGGAGCCGGCAGGGCCTGCGCAGACCTTCCATCTTGCCTGAGGGCTCTTCAGATTCAAGAGGTCCAGCCGTGGAGAAACATCCGGGACCCTCAGACACTGTTGTTTTTCGGGAGAAAAAACCAAAGGAGGTGATGGGAGGCTTTTCAAGACGCTGCTCCAAACTCATCAACTCCTCCCAGCTGCTTTACCAGGAGTATAGTGATGTTGTCCTGAATAAGGAGATCCAGAGCCAGCAGCGGCTGGAGAGCCTGTCCGAGACACCCGGGCCTAGCTCTCCGCGGCAGCCTCGGAAGGCCCTGGTCTCCTCCGAGTCGTACCTGCAGCGGCTCTCCATGGCCTCCAGCGGCTCCCTCTGGCAGGAAATCCCCGTGGTGCGCAACAGCACCGTGCTGCTCTCCATGACCCATGAAGACCAAAAGCTGCAAGAGGTCAAATTTGAGCTGATTGTGTCAGAGGCCTCCTACCTGCGCAGTCTAAACATAGCTGTGGATCATTTCCAACTTTCAACTTCACTCCGGGCCACACTTTCCAACCAGGAGCACCAATGGCTCTTCTCTCGTTTACAGGATGTGCGAGACGTCAGCGCCACGTTCCTTTCAGACCTGGAAGAGAACTTTGAGAACAATATCTTCTCCTTCCAAGTATGTGACGTAGTCCTGAACCACGCCCCAGACTTCCGCCGGGTCTACCTGCCTTATGTCACCAACCAGACCTATCAGGAACGCACCTTCCAGAGCCTGATGAATAGCAACAGCAATTTCCGGGAGGTCTTGGAGAAGCTGGAGAGCGACCCCGTCTGCCAGCGCCTTTCCCTCAAGTCCTTTCTGATTCTGCCCTTCCAACGCATCACCCGCCTCAAACTGCTGCTCCAGAACATTCTGAAGAGAACACAGCCTGGCTCCTCGGAGGAGGCAGAGGCCACGAAGGCACACCACGCCCTGGAGCAGCTGATCCGGGACTGCAATAACAATGTCCAGAGTATGCGACGGACAGAGGAGCTAATCTACCTGAGCCAGAAGATTGAGTTTGAGTGCAAAATATTCCCGCTCATTTCTCAGTCACGCTGGCTGGTGAAAAGTGGGGAGCTGACAGCCTTGGAGTTCAGTGCTTCCCCAGGGCTACGAAGGAAGCTGAACACGCGTCCAGTCCACCTGCACCTCTTCAATGACTGTCTGCTGCTGTCTCGGCCCCGAGAGGGTAGCCGATTCCTGGTATTTGACCATGCTCCCTTCTCCTCCATTCGGGGGGAAAAGTGTGAAATGAAGCTACATGGACCTCACAAAAACCTGTTCCGACTCTTTCTGCGGCAGAACACTCAGGGCGCCCAGGCCGAGTTCCTCTTCCGCACGGAGACTCAAAGTGAAAAGCTTCGGTGGATCTCAGCCTTGGCCATGCCAAGAGAGGAGTTGGACCTTCTGGAGTGTTACAACTCCCCCCAGGTACAGTGCCTTCGAGCCTACAAGCCCCGAGAGAATGATGAATTGGCACTGGAGAAAGCCGACGTGGTGATGGTGACTCAGCAGAGCAGTGACGGCTGGCTGGAGGGCGTGAGGCTCTCAGACGGGGAGCGAGGCTGGTTTCCTGTGCAGCAGGTGGAGTTCATTTCCAACCCAGAGGTCCGTGCACAGAACCTGAAGGAAGCTCATCGAGTCAAGACTGCCAAACTACAGCTGGTGGAACAGCAAGCCTAA

Amino Acid Sequence

ANKHKGWSRQGLRRPSILPEGSSDSRGPAVEKHPGPSDTVVFREKKPKEVMGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPR
QPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDV
SATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNIL
KRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLL
SRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDE
LALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU52491
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 1029-end, no useful restriction sites. Longest fragment yet obtained by RT-PCR. Possible insertion event leading to frameshift.