cDNA Clone CUL3

• Synonyms: CUL-3, PHA2E
• Plasmid: pX335 CUL3 ex4 KO sense
• Parent Plasmid: pX335
• Expressed: CUL3 ex4 KO sense guide + Cas9 D10A
• Genbank: NM_003590.4
• Species: Human

Sequence of Insert

gAGTCTATGAAGAAGATTTTG

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48903
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets sense strand in of exon 4, designed to KO CUL3 when co-transfected with antisense guide 48909. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.