cDNA Clone CCDC99

• Synonyms: Spindly, hSpindly, protein Spindly
• Plasmid: pCMV5D GFP CCDC99 K117R
• Parent Plasmid: pCMV5D GFP
• Expressed: GFP-CCDC99 K117R
• Genbank: NM_017785.4
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCATGGAGGCAGATATAATCACAAATCTTCGATGCAGGCTCAAAGAGGCTGAAGAGGAGCGACTAAAAGCTGCACAGTATGGTTTACAACTAGTAGAGAGTCAAAATGAATTACAGAATCAATTGGATAAATGTCGTAATGAAATGATGACCATGACTGAGAGTTATGAACAAGAAAAATATACCCTTCAAAGAGAAGTTGAACTCAAGAGTCGAATGTTAGAAAGTTTGAGCTGCGAATGTGAAGCTATTAAACAACAACAAAAAATGCACCTGGAGAAATTGGAAGAACAACTAAGCAGAAGCCATGGACAGGAAGTGAATGAACTAAAAACTAAGATAGAAAAACTGAGAGTGGAATTAGATGAAGCCAGGCTTAGTGAAAAGCAGCTGAAGCACCAAGTAGATCATCAGAAGGAACTCCTCTCTTGTAAATCAGAGGAACTGCGCGTAATGTCTGAACGTGTGCAGGAAAGCATGTCTTCAGAGATGCTGGCTCTTCAAATTGAGCTGACAGAAATGGAGAGTATGAAGACCACCCTCAAAGAAGAAGTGAATGAACTACAATACAGACAAGAACAGCTAGAACTTCTTATTACTAACCTAATGCGCCAGGTAGACCGGCTTAAAGAGGAAAAAGAGGAGCGAGAGAAAGAAGCAGTTTCTTACTATAATGCCCTAGAGAAAGCTCGTGTAGCAAATCAAGATCTTCAGGTACAGTTGGACCAGGCACTCCAGCAAGCCTTGGATCCCAATAGTAAAGGCAACTCTTTGTTTGCAGAGGTGGAAGATCGAAGGGCAGCAATGGAACGTCAGCTTATCAGTATGAAAGTCAAGTATCAGTCACTAAAGAAGCAAAATGTATTTAACAGAGAACAGATGCAGAGAATGAAGTTACAAATTGCCACGTTGCTACAGATGAAAGGGTCTCAAACAGAATTTGAGCAGCAGGAACGGTTGCTTGCCATGTTGGAGCAGAAGAATGGTGAAATAAAACATCTTTTAGGTGAAATTAGAAATCTGGAGAAATTTAAGAATTTATATGACAGTATGGAATCTAAGCCTTCAGTCGACTCTGGTACTCTGGAAGATAACACCTATTATACAGATTTACTTCAGATGAAGCTGGATAACTTAAACAAAGAAATTGAAAGCACTAAAGGTGAATTGTCCATACAGCGAATGAAAGCATTATTTGAGAGCCAGCGGGCTCTAGATATTGAGCGAAAACTTTTTGCAAATGAAAGATGCCTCCAGCTTTCAGAAAGTGAAAATATGAAACTGAGAGCTAAACTAGATGAATTGAAACTAAAATATGAACCTGAAGAGACAGTTGAAGTGCCTGTACTGAAAAAGAGGCGTGAGGTGCTCCCTGTGGATATAACCACCGCTAAAGATGCATGTGTCAACAACAGTGCTCTCGGGGGAGAAGTTTATCGATTACCGCCTCAGAAAGAGGAGACACAGTCCTGCCCTAACAGTTTAGAAGATAACAACTTGCAATTAGAAAAATCAGTTTCTATACACACACCAGTAGTCAGTCTCTCTCCTCACAAAAATCTGCCCGTGGATATGCAGCTGAAGAAGGAAAAGAAATGTGTGAAACTCATAGGAGTTCCCGCTGACGCTGAGGCCTTAAGTGAAAGAAGTGGAAACACCCCTAACTCTCCCAGGTTAGCTGCTGAATCAAAGCTTCAAACAGAAGTTAAAGAAGGAAAAGAAACTTCAAGCAAATTGGAAAAAGAAACTTGTAAGAAATCACACCCTATTCTATATGTGTCTTCTAAATCTACTCCAGAGACCCAGTGCCCTCAACAGTAAGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSFEEFMEAD
IITNLRCRLKEAEEERLKAAQYGLQLVESQNELQNQLDKCRNEMMTMTES
YEQEKYTLQREVELKSRMLESLSCECEAIKQQQKMHLEKLEEQLSRSHGQ
EVNELKTKIEKLRVELDEARLSEKQLKHQVDHQKELLSCKSEELRVMSER
VQESMSSEMLALQIELTEMESMKTTLKEEVNELQYRQEQLELLITNLMRQ
VDRLKEEKEEREKEAVSYYNALEKARVANQDLQVQLDQALQQALDPNSKG
NSLFAEVEDRRAAMERQLISMKVKYQSLKKQNVFNREQMQRMKLQIATLL
QMKGSQTEFEQQERLLAMLEQKNGEIKHLLGEIRNLEKFKNLYDSMESKP
SVDSGTLEDNTYYTDLLQMKLDNLNKEIESTKGELSIQRMKALFESQRAL
DIERKLFANERCLQLSESENMKLRAKLDELKLKYEPEETVEVPVLKKRRE
VLPVDITTAKDACVNNSALGGEVYRLPPQKEETQSCPNSLEDNNLQLEKS
VSIHTPVVSLSPHKNLPVDMQLKKEKKCVKLIGVPADAEALSERSGNTPN
SPRLAAESKLQTEVKEGKETSSKLEKETCKKSHPILYVSSKSTPETQCPQ
Q*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48454
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: K117R, also contains the known variations Y508H and L586S (benign according to ensembl).; ; All CCDC99 plasmids MUST be grown at 30 degrees C or less to prevent recombination.