cDNA Clone Brca1 (Mouse)

• Plasmid: pCAGGS puro 3XFLAG mouse Brca1 (P659H)
• Parent Plasmid: pCAGGS puro 3FLAG
• Expressed: 3XFLAG mouse Brca1
• Genbank: NM_009764.3 GI:161016834 with change c1976a (P659H)
• Tag: 3FLAG
• Species: Mouse

Sequence of Insert

CTCGAGCATGGATTTATCTGCCGTCCAAATTCAAGAAGTACAAAATGTCCTTCATGCTATGCAGAAAATCTTAGAGTGTCCGATCTGTTTGGAACTGATCAAAGAACCTGTTTCCACAAAGTGTGACCACATATTTTGCAAATTTTGTATGCTGAAACTTCTTAACCAGAAGAAAGGGCCTTCACAATGTCCTTTGTGTAAGAATGAGATAACCAAAAGGAGCCTACAGGGAAGCACAAGGTTTAGTCAGCTTGCTGAAGAGCTGCTGAGAATAATGGCTGCTTTTGAGCTTGACACGGGAATGCAGCTTACAAATGGTTTTAGTTTTTCAAAAAAGAGAAATAATTCTTGTGAGCGTTTGAATGAGGAGGCGTCGATCATCCAGAGCGTGGGCTACCGGAACCGTGTCAGAAGGCTTCCCCAGGTCGAACCTGGAAATGCCACCTTGAAGGACAGCCTAGGTGTCCAGCTGTCTAACCTTGGAATCGTGAGATCAGTGAAGAAAAACAGGCAGACCCAACCTCGAAAGAAATCTGTCTACATTGAACTAGACTCTGATTCTTCTGAAGAGACAGTAACTAAGCCAGGTGATTGCAGTGTGAGAGACCAGGAATTGTTACAGACCGCCCCTCAAGAAGCTGGAGATGAAGGCAAGCTGCACTCTGCAGAAGAGGCTGCTTGTGAGTTTTCTGAGGGCATAAGAAACATTGAACATCATCAATGCAGTGATGATTTAAACCCTACTGAGAATCATGCAACTGAAAGGCATCCAGAAAAATGTCAGAGTATTTCTATTTCAAATGTGTGTGTGGAGCCATGTGGCACAGATGCTCATGCCAGCTCATTACAGCCTGAGACCAGCAGTTTATTGCTCATTGAAGACAGAATGAATGCAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCATAGCAGTGAGCCAGCAGAGCAGATGGGCTGCAAGTAAAGGAACATGTAACGACAGGCAGGTTCCCAGCACTGGGGAAAAGGTAGGTCCAAACGCTGACTCCCTTAGTGATAGAGAGAAGTGGACTCACCCGCAAAGTCTGTGCCCTGAGAATTCTGGAGCTACCACCGATGTTCCTTGGATAACACTAAATAGCAGCGTTCAGAAAGTTAATGAGTGGTTTTCCAGAACTGGTGAAATGTTAACTTCTGACAGCGCATCTGCCAGGAGGCACGAGTCAAATGCTGAAGCAGCTGTTGTGTTGGAAGTTTCAAACGAAGTGGATGGGGGTTTTAGTTCTTCAAGGAAAACAGACTTAGTAACCCCCGACCCCCATCATACTTTAATGTGTAAAAGTGGAAGAGACTTCTCCAAACCAGTAGAGGATAATATCAGTGATAAAATATTTGGGAAATCCTATCAGAGAAAGGGAAGCCGCCCTCACCTGAACCATGTGACTGAAATTATAGGCACATTTATTACAGAACCACAGATAACACAAGAGCAGCCCTTCACAAATAAATTAAAACGTAAGAGAAGTACATCCCTTCAACCTGAGGACTTCATCAAGAAAGCAGATTCAGCAGGTGTTCAAAGGACTCCTGACAACATAAATCAGGGAACTGACCTAATGGAGCCAAATGAGCAAGCAGTGAGTACTACCAGTAACTGTCAGGAGAACAAAATAGCAGGTAGTAATCTCCAGAAAGAGAAAAGCGCTCATCCAACTGAATCATTGAGAAAGGAACCTGCTTCCACAGCAGGAGCCAAATCTATAAGCAACAGTGTAAGTGATTTGGAGGTAGAATTAAACGTCCACAGTTCAAAAGCACCTAAGAAAAATAGGCTGAGGAGGAAGTCTTCTATCAGGTGTGCTCTTCCACTTGAACCAATCAGTAGAAATCCAAGCCCACCTACTTGTGCTGAGCTTCAAATCGATAGTTGTGGTAGCAGTGAAGAAACAAAGAAAAACCATTCCAACCAACAGCCAGCCGGGCACCTTAGAGAGCATCAACTCATCGAAGACACTGAACCTGCAGCGGATGCCAAGAAGAACGAGCCAAATGAACACATAAGGAAGAGACGTGCCAGCGATGCTTTCCCAGAAGAGAAATTAATGAACAAAGCTGGTTTATTAACTAGCTGTTCAAGTCCTAGAAAATCTCAAGGGCCTGTCAATCCCAGCCCTCAGAGAACAGGAACAGAGCAACTTGAAACACGCCAAATGTCTGACAGTGCCAAAGAACTCGGGGATCGGGTCCTAGGAGGAGAGCCCAGTGGCAAAACCACTGACCGATCTGAGGAGAGCACCAGCGTATCCTTGGTATCTGACACTGACTACGACACTCAGAACAGTGTCTCAGTCCTGGACGCTCACACTGTCAGATATGCAAGAACAGGATCCGCTCAGTGTATGACTCAGTTTGTAGCAAGCGAAAACCCCAAGGAACTCGTCCATGGCTCTAACAATGCTGGGAGTGGCACAGAGGGTCTCAAGCCCCCCTTGAGACACGCGCTTAACCTCAGTCAGGAGAAAGTAGAAATGGAAGACAGTGAACTTGATACTCAGTATTTGCAGAATACATTTCAAGTTTCAAAGCGTCAGTCATTTGCTTTATTTTCAAAACCTAGAAGTCCCCAAAAGGACTGTGCTCACTCTGTGCCCTCAAAGGAACTGAGTCCAAAGGTGACAGCTAAAGGTAAACAAAAAGAACGTCAGGGACAGGAAGAATTTGAAATCAGTCACGTACAAGCAGTTGCGGCCACAGTGGGCTTACCTGTGCCCTGTCAAGAAGGTAAGCTAGCTGCTGATACAATGTGTGATAGAGGTTGTAGGCTTTGTCCATCATCTCATTACAGAAGCGGGGAGAATGGACTCAGCGCCACAGGTAAATCAGGAATTTCACAAAACTCACATTTTAAACAATCAGTTTCTCCCATCAGGTCATCTATAAAAACTGACAATAGGAAACCTCTGACAGAGGGACGATTTGAGAGACATACATCATCAACTGAGATGGCGGTGGGAAATGAGAACATTCTTCAGAGTACAGTGCACACAGTTAGCCTGAATAACAGAGGAAATGCTTGTCAAGAAGCCGGCTCGGGCAGTATTCATGAAGTATGTTCCACTGGTGACTCCTTCCCAGGACAACTAGGTAGAAACAGAGGGCCTAAGGTGAACACTGTGCCTCCATTAGATAGTATGCAGCCTGGTGTCTGTCAGCAAAGTGTTCCTGTAAGTGATAAGTATCTTGAAATAAAAAAGCAGGAGGGTGAGGCTGTCTGTGCAGACTTCTCTCCATGTCTATTCTCAGACCATCTTGAGCAATCTATGAGTGGTAAGGTTTTTCAGGTTTGCTCTGAGACACCTGATGACCTGCTGGATGATGTTGAAATACAGGGACATACTAGCTTTGGTGAAGGTGACATAATGGAGAGATCTGCTGTCTTTAACGGAAGCATCCTGAGAAGGGAGTCCAGTAGGAGCCCTAGTCCTGTAACCCATGCATCGAAGTCTCAGAGTCTCCACAGAGCGTCTAGGAAATTAGAATCGTCAGAAGAGAGCGACTCCACTGAGGATGAAGATCTTCCCTGCTTCCAACACTTACTGAGCAGAATAAGCAACACACCTGAGCTTACCAGATGCAGCAGTGCTGTGACACAGCGTATGCCAGAGAAAGCGGAGGGGACCCAAGCACCATGGAAGGGTAGCAGCAGTGACTGCAATAATGAGGTGATCATGATAGAGGCATCTCAGGAGCATCAGTTTAGTGAGGATCCAAGATGCTCTGGCAGCATGTTCTCTTCACAGCACAGTGCTGCCCAAGGGTCAACTGCAAATGCAAACTCCCAGGATTCCAATTTTATTCCACCTTCCAAACAGAGGAGTCACCAGTGTGGGAATGAGGAAGCTTTCCTAAGTGACAAGGAATTGATTTCAGATAACGAGGAAATGGCAACTTGCCTAGAAGAGGATAATGACCAAGAAGAGGATAGTATAATCCCAGATTCAGAGGCATCCGGATACGAGAGTGAAACAAACCTTTCTGAAGACTGCTCGCAGAGTGATATTTTAACCACTCAGCAGCGGGCGACCATGAAGTATAACCTGATAAAGCTGCAGCAGGAAATGGCTCACCTGGAAGCTGTGCTGGAGCAGCGTGGGAACCAGCCTTCTGGCCACTCCCCTTCCCTCCTAGCGGACCCTTGTGCCCTGGAAGACCTGCCAGATCTGGAACCAAACATGTCAGGAGCAGCAATTTTAACTTCAAAGAACATTAATGAGAATCCTGTAAGCCAAAATTTGAAGAGCGCTTGTGATGACAAATTCCAACTACAACATCTGGAGGGTCCCACCAGTGGAGATGACGAGTCAGGGATGGGAAGGCCTTCCCCTTTTAAATCTCCGTTGGCAGGCAGTAGGGGCTCTGCACATGGCTGCTCTAGGCATCTTCAAAAGAGAAACTCCCCCTCTCAGGAGGAGCTCCTCCAGCCTGCTGGATCAGAGGCGTCATCTGAGCCACACAATTCAACAGGGCAGTCTTGCCTGCCAAGGCGAGAGCTAGAAGGAACCCCATACCTGGGATCTGGAATCAGCCTTTTCTCTAGTAGAGACCCCGAATCTGAGTCCCCTAAAGAGCCAGCCCACATTGGCACCACACCAGCTTCAACCTCTGCACTGAAAATACCCCAAGGTCAAGTTGCTTTCCGGAGTGCAGCTGCTGCTGGTGCTGATAAAGCAGTGGTAGGAATTGTGAGCAAGATAAAGCCGGAATTGACATCTTCAGAAGAAAGAGCGGATAGAGACATATCCATGGTGGTGTCAGGCTTGACCCCCAAAGAAGTAATGACCGTGCAAAAGTTTGCTGAAAAATACCGCCTCACTTTAACTGACGCAATTACTGAGGAGACTACACATGTAATTATAAAAACAGATGCGGAGTTTGTGTGTGAGCGGACACTGAAATATTTTCTGGGCATTGCAGGAGGAAAGTGGATAGTTAGCTATTCATGGGTGGTCCGGTCTATCCAAGAAAGAAGACTTCTGAATGTGCATGAATTTGAAGTCAAAGGAGATGTTGTGACTGGAAGAAATCACCAAGGTCCAAGGCGATCCAGAGAATCCCGGGAAAAGCTCTTCAAGGGCCTACAGGTCTATTGTTGTGAGCCCTTCACCAACATGCCCAAAGATGAGCTGGAGAGGATGCTGCAGCTGTGTGGGGCTTCCGTGGTGAAGGAGCTTCCATCGCTCACCCATGACACAGGTGCTCATCTAGTTGTGATCGTGCAGCCAAGCGCCTGGACAGAAGACAGCAACTGCCCAGATATTGGGCAGCTGTGCAAGGCACGTCTTGTGATGTGGGACTGGGTGTTGGACAGTCTATCCAGCTACCGGTGTCGGGATCTGGATGCCTACCTGGTACAGAATATCACCTGTGACAGTAGTGAGCCACAAGACTCCAATGATTAAGCGGCCGC

Amino Acid Sequence

MDYKDHDGDYKDHDIDYKDDDDKGSPEFNSSMDLSAVQIQEVQNVLHAMQ
KILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEIT
KRSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLN
EEASIIQSVGYRNRVRRLPQVEPGNATLKDSLGVQLSNLGIVRSVKKNRQ
TQPRKKSVYIELDSDSSEETVTKPGDCSVRDQELLQTAPQEAGDEGKLHS
AEEAACEFSEGIRNIEHHQCSDDLNPTENHATERHPEKCQSISISNVCVE
PCGTDAHASSLQPETSSLLLIEDRMNAEKAEFCNKSKQPGIAVSQQSRWA
ASKGTCNDRQVPSTGEKVGPNADSLSDREKWTHPQSLCPENSGATTDVPW
ITLNSSVQKVNEWFSRTGEMLTSDSASARRHESNAEAAVVLEVSNEVDGG
FSSSRKTDLVTPDPHHTLMCKSGRDFSKPVEDNISDKIFGKSYQRKGSRP
HLNHVTEIIGTFITEPQITQEQPFTNKLKRKRSTSLQPEDFIKKADSAGV
QRTPDNINQGTDLMEPNEQAVSTTSNCQENKIAGSNLQKEKSAHPTESLR
KEPASTAGAKSISNSVSDLEVELNVHSSKAPKKNRLRRKSSIRCALPLEP
ISRNPSPPTCAELQIDSCGSSEETKKNHSNQQPAGHLREHQLIEDTEPAA
DAKKNEPNEHIRKRRASDAFPEEKLMNKAGLLTSCSSPRKSQGPVNPSPQ
RTGTEQLETRQMSDSAKELGDRVLGGEPSGKTTDRSEESTSVSLVSDTDY
DTQNSVSVLDAHTVRYARTGSAQCMTQFVASENPKELVHGSNNAGSGTEG
LKPPLRHALNLSQEKVEMEDSELDTQYLQNTFQVSKRQSFALFSKPRSPQ
KDCAHSVPSKELSPKVTAKGKQKERQGQEEFEISHVQAVAATVGLPVPCQ
EGKLAADTMCDRGCRLCPSSHYRSGENGLSATGKSGISQNSHFKQSVSPI
RSSIKTDNRKPLTEGRFERHTSSTEMAVGNENILQSTVHTVSLNNRGNAC
QEAGSGSIHEVCSTGDSFPGQLGRNRGPKVNTVPPLDSMQPGVCQQSVPV
SDKYLEIKKQEGEAVCADFSPCLFSDHLEQSMSGKVFQVCSETPDDLLDD
VEIQGHTSFGEGDIMERSAVFNGSILRRESSRSPSPVTHASKSQSLHRAS
RKLESSEESDSTEDEDLPCFQHLLSRISNTPELTRCSSAVTQRMPEKAEG
TQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGSMFSSQHSAAQGSTA
NANSQDSNFIPPSKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDNDQE
EDSIIPDSEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAH
LEAVLEQRGNQPSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKNINEN
PVSQNLKSACDDKFQLQHLEGPTSGDDESGMGRPSPFKSPLAGSRGSAHG
CSRHLQKRNSPSQEELLQPAGSEASSEPHNSTGQSCLPRRELEGTPYLGS
GISLFSSRDPESESPKEPAHIGTTPASTSALKIPQGQVAFRSAAAAGADK
AVVGIVSKIKPELTSSEERADRDISMVVSGLTPKEVMTVQKFAEKYRLTL
TDAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQER
RLLNVHEFEVKGDVVTGRNHQGPRRSRESREKLFKGLQVYCCEPFTNMPK
DELERMLQLCGASVVKELPSLTHDTGAHLVVIVQPSAWTEDSNCPDIGQL
CKARLVMWDWVLDSLSSYRCRDLDAYLVQNITCDSSEPQDSND*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49073
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee