cDNA Clone CCDC99

• Synonyms: Spindly, hSpindly, protein Spindly
• Plasmid: pACT2.6 CCDC99
• Parent Plasmid: pACT2.6
• Expressed: GAL4-DNA AD-HA-CCDC99
• Genbank: NM_017785.4
• Tag: GAL4 DNA-AD
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCATGGAGGCAGATATAATCACAAATCTTCGATGCAGGCTCAAAGAGGCTGAAGAAGAGCGACTAAAAGCTGCACAGTATGGTTTACAACTAGTAGAGAGTCAAAATGAATTACAGAATCAATTGGATAAATGTCGTAATGAAATGATGACCATGACTGAGAGTTATGAACAAGAAAAATATACCCTTCAAAGAGAAGTTGAACTCAAGAGTCGAATGTTAGAAAGTTTGAGCTGCGAATGTGAAGCTATTAAACAACAACAAAAAATGCACCTGGAGAAATTGGAAGAACAACTAAGCAGAAGCCATGGACAGGAAGTGAATGAACTAAAAACTAAGATAGAAAAACTGAAAGTGGAATTAGATGAAGCCAGGCTTAGTGAAAAGCAGCTGAAGCACCAAGTAGATCATCAGAAGGAACTCCTCTCTTGTAAATCAGAGGAACTGCGCGTAATGTCTGAACGTGTGCAGGAAAGCATGTCTTCAGAGATGCTGGCTCTTCAAATTGAGCTGACAGAAATGGAGAGTATGAAGACCACCCTCAAAGAAGAAGTGAATGAACTACAATACAGACAAGAACAGCTAGAACTTCTTATTACTAACCTAATGCGCCAGGTAGACCGGCTTAAAGAGGAAAAAGAGGAGCGAGAGAAAGAAGCAGTTTCTTACTATAATGCCCTAGAGAAAGCTCGTGTAGCAAATCAAGATCTTCAGGTACAGTTGGACCAGGCACTCCAGCAAGCCTTGGATCCCAATAGTAAAGGCAACTCTTTGTTTGCAGAGGTGGAAGATCGAAGGGCAGCAATGGAACGTCAGCTTATCAGTATGAAAGTCAAGTATCAGTCACTAAAGAAGCAAAATGTATTTAACAGAGAACAGATGCAGAGAATGAAGTTACAAATTGCCACGTTGCTACAGATGAAAGGGTCTCAAACTGAATTTGAGCAGCAGGAACGGTTGCTTGCCATGTTGGAGCAGAAGAATGGTGAAATAAAACATCTTTTAGGTGAAATTAGAAATCTGGAGAAATTTAAGAATTTATATGACAGTATGGAATCTAAGCCTTCAGTCGACTCTGGTACTCTGGAAGATAACACCTATTATACAGATTTACTTCAGATGAAGCTGGATAACTTAAACAAAGAAATTGAAAGCACTAAAGGTGAATTGTCCATACAGCGAATGAAAGCATTATTTGAGAGCCAGCGGGCTCTAGATATTGAGCGAAAACTTTTTGCAAATGAAAGATGCCTCCAGCTTTCAGAAAGTGAAAATATGAAACTGAGAGCTAAACTAGATGAATTGAAACTAAAATATGAACCTGAAGAGACAGTTGAAGTGCCTGTACTGAAAAAGAGGCGTGAGGTGCTCCCTGTGGATATAACCACCGCTAAAGATGCATGTGTCAACAACAGTGCTCTCGGGGGAGAAGTTTATCGATTACCGCCTCAGAAAGAGGAGACACAGTCCTGCCCTAACAGTTTAGAAGATAACAACTTGCAATTAGAAAAATCAGTTTCTATACACACACCAGTAGTCAGTCTCTCTCCTCACAAAAATCTGCCCGTGGATATGCAGCTGAAGAAGGAAAAGAAATGTGTGAAACTCATAGGAGTTCCCGCTGACGCTGAGGCCTTAAGTGAAAGAAGTGGAAACACCCCTAACTCTCCCAGGTTAGCTGCTGAATCAAAGCTTCAAACAGAAGTTAAAGAAGGAAAAGAAACTTCAAGCAAATTGGAAAAAGAAACTTGTAAGAAATCACACCCTATTCTATATGTGTCTTCTAAATCTACTCCAGAGACCCAGTGCCCTCAACAGTAAGCGGCCGC

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLI
TTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYAS
LGGHMAMGGSGEFMEADIITNLRCRLKEAEEERLKAAQYGLQLVESQNEL
QNQLDKCRNEMMTMTESYEQEKYTLQREVELKSRMLESLSCECEAIKQQQ
KMHLEKLEEQLSRSHGQEVNELKTKIEKLKVELDEARLSEKQLKHQVDHQ
KELLSCKSEELRVMSERVQESMSSEMLALQIELTEMESMKTTLKEEVNEL
QYRQEQLELLITNLMRQVDRLKEEKEEREKEAVSYYNALEKARVANQDLQ
VQLDQALQQALDPNSKGNSLFAEVEDRRAAMERQLISMKVKYQSLKKQNV
FNREQMQRMKLQIATLLQMKGSQTEFEQQERLLAMLEQKNGEIKHLLGEI
RNLEKFKNLYDSMESKPSVDSGTLEDNTYYTDLLQMKLDNLNKEIESTKG
ELSIQRMKALFESQRALDIERKLFANERCLQLSESENMKLRAKLDELKLK
YEPEETVEVPVLKKRREVLPVDITTAKDACVNNSALGGEVYRLPPQKEET
QSCPNSLEDNNLQLEKSVSIHTPVVSLSPHKNLPVDMQLKKEKKCVKLIG
VPADAEALSERSGNTPNSPRLAAESKLQTEVKEGKETSSKLEKETCKKSH
PILYVSSKSTPETQCPQQ*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU44998
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All CCDC99 plasmids MUST be grown at 30 degrees C or less to prevent recombination.