cDNA Clone Abin1

• Plasmid: pFN18-Abin1 D472N
• Parent Plasmid: pFN18a Halo
• Expressed: HALO-Abin1 D472N
• Tag: HALO
• Species: Human

Sequence of Insert

cgatcgatATGGAAGGGAGAGGACCGTACCGGATCTACGACCCTGGGGGC
AGCGTGCCCTCAGGAGAGGCATCCGCAGCTTTTGAGCGCCTAGTGAAGGA
GAATTCCCGGCTGAAGGAAAAAATGCAAGGGATAAAGATGTTAGGGGAGC
TTTTGGAAGAGTCCCAGATGGAAGCGACCAGGCTCCGGCAGAAGGCAGAG
GAGCTAGTGAAGGACAACGAGCTGCTCCCACCACCTTCTCCCTCCTTGGG
CTCCTTCGACCCCCTGGCTGAGCTCACAGGAAAGGACTCAAATGTCACAG
CATCTCCCACAGCCCCTGCATGCCCCAGTGACAAGCCAGCACCAGTCCAG
AAGCCTCCATCCAGTGGCACCTCCTCTGAATTTGAAGTGGTCACTCCTGA
GGAGCAGAATTCACCAGAGAGCAGCAGCCATGCCAATGCGATGGCGCTGG
GCCCCCTGCCCCGTGAGGACGGCAACCTGATGCTGCACCTGCAGCGCCTG
GAGACCACGCTGAGTGTGTGTGCCGAGGAGCCGGACCACGGCCAGCTCTT
CACCCACCTGGGCCGCATGGCCCTGGAGTTCAACCGACTGGCATCCAAGG
TGCACAAGAATGAGCAGCGCACCTCCATTCTGCAGACCCTGTGTGAGCAG
CTTCGGAAGGAGAACGAGGCTCTGAAGGCCAAGTTGGATAAGGGCCTGGA
ACAGCGGGATCAGGCTGCCGAGAGGCTGCGGGAGGAAAATTTGGAGCTCA
AGAAGTTGTTGATGAGCAATGGCAACAAAGAGGGTGCGTCTGGGCGGCCA
GGCTCACCGAAGATGGAAGGGACAGGCAAGAAGGCAGTGGCTGGACAGCA
GCAGGCTAGTGTGACGGCAGGTAAGGTCCCAGAGGTGGTGGCCTTGGGCG
CACCCGAGAAGAAGGTGAAGATGCTGGAGCAGCAGCGCAGTGAGCTGCTG
GAAGTGAACAAGCAGTGGGACCAGCATTTCCGGTCCATGAAGCAGCAGTA
TGAGCAGAAGATCACTGAGCTGCGTCAGAAGCTGGCTGATTTGCAGAAGC
AGGTGACTGACCTGGAGGCCGAGCGGGAGCAGAAGCAGCGTGACTTTGAC
CGCAAGCTCCTCCTGGCCAAGTCCAAGATTGAAATGGAGGAGACCGACAA
GGAGCAGCTGACAGCAGAGGCCAAGGAGCTGCGCCAAAAGGTCAAGTACC
TGCAGGATCAGCTGAGCCCACTCACCCGACAGCGTGAGTACCAGGAAAAG
GAGATCCAGCGGCTCAACAAGGCCCTGGAGGAAGCACTGAGCATCCAAAC
CCCGCCATCATCTCCACCAACAGCATTTGGGAGCCCAGAAGGAGCAGGGG
CCCTCCTAAGGAAACAGGAGCTGGTCACGCAGAATGAGTTGCTGAAACAG
CAGGTGAAGATCTTCGAGGAGaACTTCCAGAGGGAGCGCAGTGATCGTGA
GCGCATGAATGAGGAGAAGGAAGAGCTGAAGAAGCAAGTGGAGAAGCTGC
AGGCCCAGGTCACCCTGTCAAATGCCCAGCTAAAAGCATTCAAAGATGAG
GAGAAGGCAAGAGAAGCCCTCAGACAGCAGAAGAGGAAAGCAAAGGCCTC
AGGAGAGCGTTACCATGTGGAGCCCCACCCAGAACATCTCTGCGGGGCCT
ACCCCTACGCCTACCCGCCCATGCCAGCCATGGTGCCACACCATGGCTTC
GAGGACTGGTCCCAGATCCGCTACCCCCCTCCCCCCATGGCCATGGAGCA
CCCGCCCCCACTCCCCAACTCGCGCCTCTTCCATCTGCCGGAATACACCT
GGCGTCTACCTTGTGGAGGGGTTCGAAATCCAAATCAGAGCTCCCAAGTG
ATGGACCCTCCCACAGCCAGGCCTACAGAACCAGAGTCTCCAAAAAATGA
CCGTGAGGGGCCTCAGTGAGGATCC

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRN
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGEPT
TEDLYFQSDNAIDMEGRGPYRIYDPGGSVPSGEASAAFERLVKENSRLKE
KMQGIKMLGELLEESQMEATRLRQKAEELVKDNELLPPPSPSLGSFDPLA
ELTGKDSNVTASPTAPACPSDKPAPVQKPPSSGTSSEFEVVTPEEQNSPE
SSSHANAMALGPLPREDGNLMLHLQRLETTLSVCAEEPDHGQLFTHLGRM
ALEFNRLASKVHKNEQRTSILQTLCEQLRKENEALKAKLDKGLEQRDQAA
ERLREENLELKKLLMSNGNKEGASGRPGSPKMEGTGKKAVAGQQQASVTA
GKVPEVVALGAPEKKVKMLEQQRSELLEVNKQWDQHFRSMKQQYEQKITE
LRQKLADLQKQVTDLEAEREQKQRDFDRKLLLAKSKIEMEETDKEQLTAE
AKELRQKVKYLQDQLSPLTRQREYQEKEIQRLNKALEEALSIQTPPSSPP
TAFGSPEGAGALLRKQELVTQNELLKQQVKIFEENFQRERSDRERMNEEK
EELKKQVEKLQAQVTLSNAQLKAFKDEEKAREALRQQKRKAKASGERYHV
EPHPEHLCGAYPYAYPPMPAMVPHHGFEDWSQIRYPPPPMAMEHPPPLPN
SRLFHLPEYTWRLPCGGVRNPNQSSQVMDPPTARPTEPESPKNDREGPQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU23868
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 39108 backbone (pFN18 cut Pvu BAMH1)