cDNA Clone Cullin4a

• Plasmid: pFastbacDual DAC TEV Cullin4a, Rbx1
• Parent Plasmid: pFastbacDual DAC TEV
• Expressed: DAC-Cullin4a
• Genbank: NM_001008895
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCATGGCGGACGAGGCCCCGCGGAAGGGCAGCTTCTCGGCGCTCGTGGGCCGCACCAACGGCCTCACCAAGCCCGCGGCCCTGGCCGCCGCGCCCGCCAAGCCGGGGGGCGCGGGCGGCTCCAAGAAGCTGGTCATCAAGAACTTCCGAGACAGACCTCGGCTGCCCGACAACTACACGCAGGACACGTGGCGGAAGCTGCACGAGGCGGTGCGGGCCGTGCAGAGCAGCACCTCCATCAGGTACAACCTCGAGGAGCTCTACCAGGCTGTGGAAAATCTCTGTTCTCACAAAGTCTCCCCAATGCTCTACAAGCAACTGCGTCAGGCCTGTGAAGACCACGTCCAGGCACAGATCCTTCCGTTTAGAGAAGACTCACTAGATAGTGTTTTATTTTTAAAGAAGATTAACACGTGCTGGCAGGACCACTGCAGACAAATGATCATGATCAGAAGCATCTTCCTGTTCTTGGACCGCACCTATGTGCTGCAGAACTCCACGCTGCCCTCCATCTGGGATATGGGATTAGAACTGTTTAGAACCCATATTATTAGTGATAAAATGGTTCAGAGTAAAACCATTGATGGAATCCTACTGCTGATCGAGCGCGAGAGGAGCGGCGAGGCCGTGGACCGGAGCCTGTTGCGGAGCCTCCTGGGCATGCTGTCTGACCTGCAGGTGTATAAAGATTCATTTGAACTGAAATTTTTGGAAGAGACTAATTGCTTATATGCTGCCGAAGGCCAAAGGTTAATGCAGGAAAGAGAGGTTCCAGAATATCTTAACCATGTAAGTAAACGCTTAGAGGAAGAGGGAGACAGAGTAATCACTTACTTGGACCACAGCACACAGAAACCACTGATTGCTTGTGTGGAGAAACAGCTATTAGGAGAACATTTAACAGCAATTCTGCAGAAAGGGCTCGACCACTTACTGGATGAGAACAGAGTGCCGGACCTCGCACAGATGTACCAGCTGTTCAGCCGGGTGAGGGGCGGGCAGCAGGCGCTGCTGCAGCACTGGAGCGAGTACATCAAGACTTTTGGAACAGCGATCGTAATCAATCCTGAGAAAGACAAAGACATGGTCCAAGACCTGTTGGACTTCAAGGACAAGGTGGACCACGTGATCGAGGTCTGCTTCCAGAAGAATGAGCGGTTCGTCAACCTGATGAAGGAGTCCTTTGAGACGTTCATCAACAAGAGACCCAACAAGCCTGCAGAACTGATCGCAAAGCATGTGGATTCAAAGTTAAGAGCAGGCAACAAAGAAGCCACAGACGAGGAGCTGGAGCGGACGTTGGACAAGATCATGATCCTGTTCAGGTTTATCCACGGTAAAGATGTCTTTGAAGCATTTTATAAAAAAGATTTGGCAAAAAGACTCCTTGTTGGGAAAAGTGCCTCAGTCGATGCTGAAAAGTCTATGTTGTCAAAGCTCAAGCATGAGTGCGGTGCAGCCTTCACCAGCAAGCTGGAAGGCATGTTCAAGGACATGGAGCTTTCGAAGGACATCATGGTTCATTTCAAGCAGCATATGCAGAATCAGAGTGACTCAGGCCCTATAGACCTCACAGTGAACATACTCACAATGGGCTACTGGCCAACATACACGCCCATGGAAGTGCACTTAACCCCAGAAATGATTAAACTTCAGGAAGTATTTAAGGCATTTTATCTTGGAAAGCACAGTGGTCGAAAACTTCAGTGGCAAACTACTTTGGGACATGCTGTTTTAAAAGCGGAGTTTAAAGAAGGGAAGAAGGAATTCCAGGTGTCCCTCTTCCAGACACTGGTGCTCCTCATGTTCAACGAGGGAGATGGCTTCAGCTTTGAGGAGATAAAAATGGCCACGGGGATAGAGGATAGTGAATTGCGCAGAACGCTGCAGTCCCTGGCCTGTGGCAAAGCACGTGTGCTGATTAAAAGTCCCAAAGGAAAGGAAGTGGAAGATGGAGACAAGTTCATTTTTAATGGAGAGTTCAAGCACAAGTTGTTTAGAATAAAGATCAATCAAATTCAGATGAAGGAAACTGTTGAGGAACAGGTTAGCACCACTGAGAGAGTGTTTCAGGATAGACAATATCAGATTGATGCTGCTATCGTCAGAATAATGAAGATGAGAAAGACTCTTGGTCATAATCTTCTAGTTTCTGAATTATATAATCAGCTGAAATTTCCAGTAAAGCCTGGAGATTTGAAAAAGAGAATTGAATCTCTGATAGACAGAGACTATATGGAGAGAGACAAAGACAATCCGAATCAGTACCACTACGTGGCCTGAGCGGCCGC

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPENLYFQGGSMADEAPRKGSFSALVGRTNGLTKPAALAAAPAKPGGAGGSKKLVIKNFRDRPRLPDNYTQDTWRKLHEAVRAVQSSTSIRYNLEELYQAVENLCSHKVSPMLYKQLRQACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSTLPSIWDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLLGMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGLDHLLDENRVPDLAQMYQLFSRVRGGQQALLQHWSEYIKTFGTAIVINPEKDKDMVQDLLDFKDKVDHVIEVCFQKNERFVNLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERTLDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSDSGPIDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRKLQWQTTLGHAVLKAEFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNGEFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU23656
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cullin4a subcloned into SC23165 (pFBDual-DAC_TEV_ with Rbx1 in p10F cassette and empty DAC cassette). TEV not blunt