cDNA Clone Cullin2

• Plasmid: pCMV Cullin2-FLAG
• Parent Plasmid: pCMV5 C-FLAG
• Expressed: Cullin2-FLAG
• Genbank: NM_003591
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTCTTTGAAACCAAGAGTAGTAGATTTTGATGAAACATGGAACAAACTTTTGACGACAATAAAAGCCGTGGTCATGTTGGAATACGTCGAAAGAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCCTATCCTGAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTTGGAAAATCATGTTCGGCATTTGCATAAGAGAGTTTTGGAGTCAGAAGAACAAGTACTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGCAGACTATATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAAGAATAAATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATATGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGGAAATTGATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCGAGAAATCAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTCCCCTTA
AAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAACAGGAGA
GTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACTGCTCAC
AGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATTCGATGT
CGAAAATACCTACATCCAAGTTCATATACTAAGGTGATTCATGAATGTCA
ACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAATGTCATA
ATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTACGTCTTA
CTCCGTGCTGTGTCCACTGGTTTACCTCATATGATTCAGGAGCTGCAAAA
CCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACTCAGGAAA
ACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGTAAATTT
GTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGC
GTTGGATAAGGCCCTTACGTCAGTTGTAAATTACAGAGAACCTAAGTCTG
TTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAACTTACTGAAG
AAGTCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCTCACGAG
CTTCATCACAGTGTTCAAATACATTGATGACAAGGACGTCTTTCAAAAGT
TCTACGCAAGAATGCTGGCAAAACGTTTAATTCATGGGTTATCCATGTCT
ATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTGTGGTTA
TGAGTTTACCAGCAAGCTACATCGGATGTATACAGATATGAGTGTCAGCG
CTGATCTCAACAATAAGTTCAACAATTTTATCAAAAACCAAGACACAGTA
ATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGGCTGGTGCGTG
GCCTCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGGAATTAG
AAAAAAGTGTACAGATGTTTGAATTATTTTATAGCCAACATTTCAGTGGA
AGGAAACTTACATGGTTACATTATCTGTGTACAGGTGAAGTTAAAATGAA
CTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAATGGCAG
TTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGTTATAAAGAGCTTCAG
GACAGCACTCAGATGAATGAAAAGGAACTGACAAAAACAATCAAATCATT
ACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAAGATATTGATG
CAGAATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGAACAAAA
TTTAAAATTACTACATCAATGCAGAAAGACACACCACAAGAAATGGAGCA
GACTAGAAGTGCAGTTGATGAGGACCGGAAAATGTATCTCCAAGCTGCTA
TAGTTCGTATCATGAAAGCACGAAAAGTGCTTCGGCACAATGCCCTTATT
CAAGAGGTGATTAGCCAGTCAAGAGCTAGGTTTAATCCCAGTATCAGCAT
GATTAAGAAGTGTATTGAAGTTCTGATAGACAAACAATACATAGAACGCA
GCCAGGCGTCGGCAGATGAATACAGCTACGTCGCGGCGGCCGC

Amino Acid Sequence

MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYP
EPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADY
MDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKL
MVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPL
KFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRC
RKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVL
LRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKF
VQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLK
KSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMS
MDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTV
IDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSG
RKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQ
DSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTK
FKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALI
QEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVAAAADY
KDDDDK**

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20495
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C-terminally FLAG tagged Cullin2 for mammalian expression; ; Estimated MW of expressed protein : 88192 Daltons