cDNA Clone CLK1

• Plasmid: pGEX6P-3-CLK1 D325A
• Parent Plasmid: pGEX6P3
• Expressed: GST-CLK1 D325A
• Genbank: BC031549
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGAGACACTCAAAGAGAACTTACTGTCCTGATTGGGATGACAAGGATTGGGATTATGGAAAATGGAGGAGCAGCAGCAGTCATAAAAGAAGGAAGAGATCACATAGCAGTGCCCAGGAGAACAAGCGCTGCAAATACAATCACTCTAAAATGTGTGATAGCCATTATTTGGAAAGCAGGTCTATAAATGAGAAAGATTATCATAGTCGACGCTACATTGATGAGTACAGAAATGACTACACTCAAGGATGTGAACCTGGACATCGCCAAAGAGACCATGAAAGCCGGTATCAGAACCAT
AGTAGCAAGTCTTCTGGTAGAAGTGGAAGAAGTAGTTATAAAAGCAAACA
CAGGATTCACCACAGTACTTCACATCGTCGTTCACATGGGAAGAGTCACC
GAAGGAAAAGAACCAGGAGTGTAGAGGATGATGAGGAGGGTCACCTGATC
TGTCAGAGTGGAGACGTACTAAGTGCAAGATATGAAATTGTTGATACTTT
AGGTGAAGGAGCTTTTGGAAAAGTTGTGGAGTGCATCGATCATAAAGCGG
GAGGTAGACATGTAGCAGTAAAAATAGTTAAAAATGTGGATAGATACTGT
GAAGCTGCTCGCTCAGAAATACAAGTTCTGGAACATCTGAATACAACAGA
CCCCAACAGTACTTTCCGCTGTGTCCAGATGTTGGAATGGTTTGAGCATC
ATGGTCACATTTGCATTGTTTTTGAACTATTGGGACTTAGTACTTACGAC
TTCATTAAAGAAAATGGTTTTCTACCATTTCGACTGGATCATATCAGAAA
GATGGCATATCAGATATGCAAGTCTGTGAATTTTTTGCACAGTAATAAGT
TGACTCACACAGACTTAAAGCCTGAAAACATCTTATTTGTGCAGTCTGAC
TACACAGAGGCGTATAATCCCAAAATAAAACGTGATGAACGCACCTTAAT
AAATCCAGATATTAAAGTTGTAGCCTTTGGTAGTGCAACATATGATGACG
AACATCACAGTACATTGGTATCTACAAGACATTATAGAGCACCTGAAGTT
ATTTTAGCCCTAGGGTGGTCCCAACCATGTGATGTCTGGAGCATAGGATG
CATTCTTATTGAATACTATCTTGGGTTTACCGTATTTCCAACACACGATA
GTAAGGAGCATTTAGCAATGATGGAAAGGATTCTTGGACCTCTACCAAAA
CATATGATACAGAAAACCAGGAAACGTAAATATTTTCACCACGATCGATT
AGACTGGGATGAACACAGTTCTGCCGGCAGATATGTTTCAAGACGCTGTA
AACCTCTGAAGGAATTTATGCTTTCTCAAGATGTTGAACATGAGCGTCTC
TTTGACCTCATTCAGAAAATGTTGGAGTATGATCCAGCCAAAAGAATTAC
TCTCAGAGAAGCCTTAAAGCATCCTTTCTTTGACCTTCTGAAGAAAAGTA
TATAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMRHSKRTYCPDWDDKDWDY
GKWRSSSSHKRRKRSHSSAQENKRCKYNHSKMCDSHYLESRSINEKDYHS
RRYIDEYRNDYTQGCEPGHRQRDHESRYQNHSSKSSGRSGRSSYKSKHRI
HHSTSHRRSHGKSHRRKRTRSVEDDEEGHLICQSGDVLSARYEIVDTLGE
GAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPN
STFRCVQMLEWFEHHGHICIVFELLGLSTYDFIKENGFLPFRLDHIRKMA
YQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNPKIKRDERTLINP
DIKVVAFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCIL
IEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDW
DEHSSAGRYVSRRCKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRITLR
EALKHPFFDLLKKSI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16984
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mutagenesis of pGEX6P-3 CLK1 (DU5187); Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 84061 Daltons