cDNA Clone BRK

• Synonyms: PTK6
• Plasmid: pGEX6P-1-BRK kinase domain (H180-T445)
• Parent Plasmid: pGEX6P2
• Expressed: GST-BRK kinase domain (H180-T445)
• Genbank: NM_005975
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCATTGGGATGACTGGGAGAGGCCGAGGGAGGAGTTCACGCTCTG
CAGGAAGCTGGGGTCCGGCTACTTTGGGGAGGTCTTCGAGGGGCTCTGGA
AAGACCGGGTCCAGGTGGCCATTAAGGTGATTTCTCGAGACAACCTCCTG
CACCAGCAGATGCTGCAGTCGGAGATCCAGGCCATGAAGAAGCTGCGGCA
CAAACACATCCTGGCGCTGTACGCCGTGGTGTCCGTGGGGGACCCCGTGT
ACATCATCACGGAGCTCATGGCCAAGGGCAGCCTGCTGGAGCTGCTCCGC
GACTCTGATGAGAAAGTCCTGCCCGTTTCGGAGCTGCTGGACATCGCCTG
GCAGGTGGCTGAGGGCATGTGTTACCTGGAGTCGCAGAATTACATCCACC
GGGACCTGGCCGCCAGGAACATCCTCGTCGGGGAAAACACCCTCTGCAAA
GTTGGGGACTTCGGGTTAGCCAGGCTTATCAAGGAGGACGTCTACCTCTC
CCATGACCACAATATCCCCTACAAGTGGACGGCCCCTGAAGCGCTCTCCC
GAGGCCATTACTCCACCAAATCCGACGTCTGGTCCTTTGGGATTCTCCTG
CATGAGATGTTCAGCAGGGGTCAGGTGCCCTACCCAGGCATGTCCAACCA
TGAGGCCTTCCTGAGGGTGGACGCCGGCTACCGCATGCCCTGCCCTCTGG
AGTGCCCGCCCAGCGTGCACAAGCTGATGCTGACATGCTGGTGCAGGGAC
CCCGAGCAGAGACCCTGCTTCAAGGCCCTGCGGGAGAGGCTCTCCAGCTT
CACCTGAAGCTTGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSHWDDWERPREEFTLCRKLG
SGYFGEVFEGLWKDRVQVAIKVISRDNLLHQQMLQSEIQAMKKLRHKHIL
ALYAVVSVGDPVYIITELMAKGSLLELLRDSDEKVLPVSELLDIAWQVAE
GMCYLESQNYIHRDLAARNILVGENTLCKVGDFGLARLIKEDVYLSHDHN
IPYKWTAPEALSRGHYSTKSDVWSFGILLHEMFSRGQVPYPGMSNHEAFL
RVDAGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31503
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 57572 Daltons