cDNA Clone BS69

• Plasmid: pEBG-2T-BS69
• Parent Plasmid: pEBG2T
• Expressed: GST-BS69
• Genbank: NM_006624.2 GI:47078241
• Tag: GST
• Species: Human

Sequence of Insert

ggatccTCTCGAGTCCACGGTATGCACCCTAAAGAGACCACCCGTCAGCTGAGCTTAGCTGTGAAAGATGGTCTTATTGTCGAAACTCTAACAGTGGGCTGCAAAGGTTCAAAAGCTGGTATTGAACAAGAAGGATATTGGTTGCCAGGAGAT
GAGATTGACTGGGAAACAGAAAATCATGACTGGTATTGTTTTGAATGCCATTTGCCTGGAGAGGTGTTGATATGTGACCTGTGTTTTCGTGTGTATCATTCCAAGTGTTTGTCTGATGAGTTCAGGCTTAGAGACAGCAGTAGTCCCTGGCAGTGCCCAGTTTGCAGGAGCATTAAGAAGAAGAATACAAACAAACAGGAGATGGGCACATACCTCAGATTCATTGTCTCCCGCATGAAGGAGAGGGCTATAGATCTTAATAAAAAGGGGAAGGACAATAAACACCCGATGTACAGGAGGCTGGTGCACTCAGCTGTGGACGTTCCCACCATTCAAGAGAAAGTGAATGAAGGGAAATACCGAAGTTATGAAGAGTTCAAAGCTGATGCCCAATTGCTTCTCCACAATACCGTGATTTTCTATGGAGCAGACAGTGAGCAAGCTGACATTGCGAGGATGCTATATAAAGACACATGTCATGAGCTGGATGAACTGCAGCTTTGCAAGAATTGCTTTTACTTGTCAAATGCTCGTCCTGACAACTGGTTCTGTTATCCTTGTATACCTAATCATGAGCTGGTTTGGGCTAAAATGAAAGGTTTTGGGTTTTGGCCAGCCAAAGTCATGCAGAAAGAAGACAATCAAGTCGACGTTCGCTTCTTTGGCCACCACCACCAGAGGGCCTGGATTCCTTCTGAAAACATTCAAGATATCACAGTCAACATTCATCGGCTGCACGTGAAGCGCAGTATGGGTTGGAAAAAGGCCTGTGATGAGCTGGAGCTGCATCAGCGTTTCCTACGAGAAGGGAGATTTTGGAAATCTAAGAATGAGGACCGAGGTGAGGAAGAGGCAGAATCCAGTATCTCCTCCACCAGTAATGAGCAGCTAAAGGTCACTCAAGAACCAAGAGCAAAGAAAGGACGACGTAATCAAAGTGTGGAGCCCAAAAAGGAAGAACCAGAGCCTGAAACAGAAGCAGTAAGTTCTAGCCAGGAAATACCCACGATGCCTCAGCCCATCGAAAAAGTCTCCGTGTCAACTCAGACAAAGAAGTTAAGTGCCTCTTCACCAAGAATGCTGCATCGGAGCACCCAGACCACAAACGACGGCGTGTGTCAGAGCATGTGCCATGACAAATACACCAAGATCTTCAATGACTTCAAAGACCGGATGAAGTCGGACCACAAGCGGGAGACAGAGCGTGTTGTCCGAGAAGCTCTGGAGAAGCTGCGTTCTGAAATGGAAGAAGAAAAGAGACAAGCTGTAAATAAAGCTGTAGCCAACATGCAGGGTGAGATGGACAGAAAATGTAAGCAAGTAAAGGAAAAGTGTAAGGAGGAATTTGTAGAAGAAATCAAGAAGCTGGCAACACAGCACAAGCAACTGATTTCTCAGACCAAGAAGAAGCAGTGGTGCTACAACTGTGAGGAGGAGGCCATGTACCACTGCTGCTGGAACACATCCTACTGCTCCATCAAGTGCCAGCAGGAGCACTGGCACGCGGAGCACAAGCGCACCTGCCGCCGGAAAAGATGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSSRVHGMHPKETTRQLSLAVKDGLI
VETLTVGCKGSKAGIEQEGYWLPGDEIDWETENHDWYCFECHLPGEVLIC
DLCFRVYHSKCLSDEFRLRDSSSPWQCPVCRSIKKKNTNKQEMGTYLRFI
VSRMKERAIDLNKKGKDNKHPMYRRLVHSAVDVPTIQEKVNEGKYRSYEE
FKADAQLLLHNTVIFYGADSEQADIARMLYKDTCHELDELQLCKNCFYLS
NARPDNWFCYPCIPNHELVWAKMKGFGFWPAKVMQKEDNQVDVRFFGHHH
QRAWIPSENIQDITVNIHRLHVKRSMGWKKACDELELHQRFLREGRFWKS
KNEDRGEEEAESSISSTSNEQLKVTQEPRAKKGRRNQSVEPKKEEPEPET
EAVSSSQEIPTMPQPIEKVSVSTQTKKLSASSPRMLHRSTQTTNDGVCQS
MCHDKYTKIFNDFKDRMKSDHKRETERVVREALEKLRSEMEEEKRQAVNK
AVANMQGEMDRKCKQVKEKCKEEFVEEIKKLATQHKQLISQTKKKQWCYN
CEEEAMYHCCWNTSYCSIKCQQEHWHAEHKRTCRRKR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1473
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express BS69 with N-terminal GST