cDNA Clone CK1 epsilon

• Synonyms: CSNK1E
• Plasmid: pGEX6P-2-CK1 epsilon
• Parent Plasmid: pGEX6P2
• Expressed: GST-CK1 epsilon
• Genbank: NM_152221
• Tag: GST
• Species: Human

Sequence of Insert

gcggccgcgATGGAGCTACGTGTGGGGAACAAGTACCGCCTGGGACGGAAGATCGGGAGCGGGTCCTTCGGAGATATCTACCTGGGTGCCAACATCGCCTCTGGTGAGGAAGTCGCCATCAAGCTGGAGTGTGTGAAGACAAAGCACCCCCAGCTGCACATCGAGAGCAAGTTCTACAAGATGATGCAGGGTGGCGTGGGGATCCCGTCCATCAAGTGGTGCGGAGCTGAGGGCGACTACAACGTGATGGTCATGGAGCTGCTGGGGCCTAGCCTCGAGGACCTGTTCAACTTCTGTTCCCGCAAATTCAGCCTCAAGACGGTGCTGCTCTTGGCCGACCAGATGATCAGCCGCATCGAGTATATCCACTCCAAGAACTTCATCCACCGGGACGTCAAGCCCGACAACTTCCTCATGGGGCTGGGGAAGAAGGGCAACCTGGTCTACATCATCGACTTC
GGCCTGGCCAAGAAGTACCGGGACGCCCGCACCCACCAGCACATTCCCTA
CCGGGAAAACAAGAACCTGACCGGCACGGCCCGCTACGCTTCCATCAACA
CGCACCTGGGCATTGAGCAAAGCCGTCGAGATGACCTGGAGAGCCTGGGC
TACGTGCTCATGTACTTCAACCTGGGCTCCCTGCCCTGGCAGGGGCTCAA
AGCAGCCACCAAGCGCCAGAAGTATGAACGGATCAGCGAGAAGAAGATGT
CAACGCCCATCGAGGTCCTCTGCAAAGGCTATCCCTCCGAATTCTCAACA
TACCTCAACTTCTGCCGCTCCCTGCGGTTTGACGACAAGCCCGACTACTC
TTACCTACGTCAGCTCTTCCGCAACCTCTTCCACCGGCAGGGCTTCTCCT
ATGACTACGTCTTTGACTGGAACATGCTGAAATTCGGTGCAGCCCGGAAT
CCCGAGGATGTGGACCGGGAGCGGCGAGAACACGAACGCGAGGAGAGGAT
GGGGCAGCTACGGGGGTCCGCGACCCGAGCCCTGCCCCCTGGCCCACCCA
CGGGGGCCACTGCCAACCGGCTCCGCAGTGCCGCCGAGCCCGTGGCTTCC
ACGCCAGCCTCCCGCATCCAGCCGGCTGGCAATACTTCTCCCAGAGCGAT
CTCGCGGGTCGACCGGGAGAGGAAGGTGAGTATGAGGCTGCACAGGGGTG
CGCCCGCCAACGTCTCCTCCTCAGACCTCACTGGGCGGCAAGAGGTCTCC
CGGATCCCAGCCTCACAGACAAGTGTGCCATTTGACCATCTCGGGAAGTG
Agcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEIPGSTRAAAMELRVGNK
YRLGRKIGSGSFGDIYLGANIASGEEVAIKLECVKTKHPQLHIESKFYKM
MQGGVGIPSIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLL
ADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRD
ARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNL
GSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFSTYLNFCRSL
RFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDVDRER
REHEREERMGQLRGSATRALPPGPPTGATANRLRSAAEPVASTPASRIQP
AGNTSPRAISRVDRERKVSMRLHRGAPANVSSSDLTGRQEVSRIPASQTS
VPFDHLGK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5127
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 75142 Daltons