cDNA Clone 53BP1

• Synonyms: TP53BP1
• Plasmid: pCMV5-HA 53BP1 420-1560.NLS
• Parent Plasmid: pCMV5
• Expressed: HA-53BP1 420-1560-NLS
• Genbank: NM_005657
• Tag: HA

Sequence of Insert

ggtaccGCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCCCCCTCTCCTGCCTGAGTCCACTGTATCACCACAAGCCTCAACACCAATATCTCAGAGCACACCAGTCTTCCCTCCTGGGTCACTTCCTATCCCATCCCAGCCTCAGTTTTCTCATGACATTTTTATTCCTTCCCCAAGTCTGGAAGAACAATCAAATGATGGGAAGAAAGATGGAGATATGCATAGTTCATCTTTGACAGTTGAGTGTTCTAAAACTTCAGAGATTGAACCAAAGAATTCCCCTGAGGATCTTGGGCTATCTTTGACAGGGGATTCTTGCAAGTTGATGCTTTCTACAAGTGAATATAGTCAGTCCCCAAAGATGGAGAGCTTGAGTTCTCACAGAATTGATGAAGATGGAGAAAACACACAGATTGAGGATACGGAACCCATGTCTCCAGTTCTCAATTCTAAATTTGTTCCTGCTGAAAATGATAGTATCCTGATGAATCCAGCACAGGATGGTGAAGTACAACTGAGTCAGAATGATGACAAAACAAAGGGAGATGATACAGACACCAGGGATGACATTAGTATTTTAGCCACTGGTTGCAAGGGCAGAGAAGAAACGGTAGCAGAAGATGTTTGTATTGATCTCACTTGTGATTCGGGGAGTCAGGCAGTTCCGTCACCAGCTACTCGATCTGAGGCACTTTCTAGTGTGTTAGATCAGGAGGAAGCTATGGAAATTAAAGAACACCATCCAGAGGAGGGGTCTTCAGGGTCTGAGGTGGAAGAAATCCCTGAGACACCTTGTGAAAGTCAAGGAGAGGAACTCAAAGAAGAAAATATGGAGAGTGTTCCGTTGCACCTTTCTCTGACTGAAACTCAGTCCCAAGGGTTGTGTCTTCAAAAGGAAATGCCAAAAAAAGAATGCTCAGAAGCTATGGAAGTTGAAACCAGTGTGATTAGTATTGATTCCCCTCAAAAGTTGGCAATACTTGACCAAGAATTGGAACATAAGGAACAGGAAGCTTGGGAAGAAGCTACTTCAGAGGACTCCAGTGTTGTCATTGTAGATGTGAAAGAGCCATCTCCCAGAGTTGATGTTTCTTGTGAACCTTTGGAGGGAGTGGAGAAGTGCTCAGATTCCCAGTCATGGGAGGATATTGCTCCAGAAATAGAACCATGTGCTGAGAATAGATTAGACACCAAGGAAGAAAAGAGTGTAGAATATGAAGGAGATCTGAAATCAGGGACTGCAGAAACAGAACCTGTAGAGCAAGATTCTTCACAGCCTTCCTTACCTTTAGTGAGAGCAGATGATCCTTTAAGACTTGACCAGGAGTTGCAGCAGCCCCAAACTCAGGAGAAAACAAGTAATTCATTAACAGAAGACTCAAAAATGGCTAATGCAAAGCAGCTAAGCTCAGATGCAGAGGCCCAGAAGCTGGGGAAGCCCTCTGCCCATGCCTCACAAAGCTTCTGTGAAAGTTCTAGTGAAACCCCATTTCATTTCACTTTGCCTAAAGAAGGTGATATCATCCCACCATTGACTGGTGCAACCCCACCTCTTATTGGGCACCTAAAATTGGAGCCCAAGAGACACAGTACTCCTATTGGTATTAGCAACTATCCAGAAAGCACCATAGCAACCAGTGATGTCATGTCTGAAAGCATGGTGGAGACCCATGATCCCATACTTGGGAGTGGAAAAGGGGATTCTGGGGCTGCCCCAGACGTGGATGATAAATTATGTCTAAGAATGAAACTGGTTAGTCCTGAGACTGAGGCGAGTGAAGAGTCTTTGCAGTTCAACCTGGAAAAGCCTGCAACTGGTGAAAGAAAAAATGGATCTACTGCTGTTGCTGAGTCTGTTGCCAGTCCCCAGAAGACCATGTCTGTGTTGAGCTGTATCTGTGAAGCCAGGCAAGAGAATGAGGCTCGAAGTGAGGATCCCCCCACCACACCCATCAGGGGGAACTTGCTCCACTTTCCAAGTTCTCAAGGAGAAGAGGAGAAAGAAAAATTGGAGGGTGACCATACAATCAGGCAGAGTCAACAGCCTATGAAGCCCATTAGTCCTGTCAAGGACCCTGTTTCTCCTGCTTCCCAGAAGATGGTCATACAAGGGCCATCCAGTCCTCAAGGAGAGGCAATGGTGACAGATGTGCTAGAAGACCAGAAAGAAGGACGGAGTACTAATAAGGAAAATCCTAGTAAGGCCTTGATTGAAAGGCCCAGCCAAAATAACATAGGAATCCAAACCATGGAGTGTTCCTTGAGGGTCCCAGAAACTGTTTCAGCA
GCAACCCAGACTATAAAGAATGTGTGTGAGCAGGGGACCAGTACAGTGGA
CCAGAACTTTGGAAAGCAAGATGCCACAGTTCAGACTGAGAGGGGGAGTG
GTGAGAAACCAGTCAGTGCTCCTGGGGATGATACAGAGTCGCTCCATAGC
CAGGGAGAAGAAGAGTTTGATATGCCTCAGCCTCCACATGGCCATGTCTT
ACATCGTCACATGAGAACAATCCGGGAAGTACGCACACTTGTCACTCGTG
TCATTACAGATGTGTATTATGTGGATGGAACAGAAGTAGAAAGAAAAGTA
ACTGAGGAGACTGAAGAGCCAATTGTAGAGTGTCAGGAGTGTGAAACTGA
AGTTTCCCCTTCACAGACTGGGGGCTCCTCAGGTGACCTGGGGGATATCA
GCTCCTTCTCCTCCAAGGCATCCAGCTTACACCGCACATCAAGTGGGACA
AGTCTCTCAGCTATGCACAGCAGTGGAAGCTCAGGGAAAGGAGCCGGACC
ACTCAGAGGGAAAACCAGCGGGACAGAACCCGCAGATTTTGCCTTACCCA
GCTCCCGAGGAGGCCCAGGAAAACTGAGTCCTAGAAAAGGGGTCAGTCAG
ACAGGGACGCCAGTGTGTGAGGAGGATGGTGATGCAGGCCTTGGCATCAG
ACAGGGAGGGAAGGCTCCAGTCACGCCTCGTGGGCGTGGGCGAAGGGGCC
GCCCACCTTCTCGGACCACTGGAACCAGAGAAACAGCTGTGCCTGGCCCC
TTGGGCATAGAGGACATTTCACCTAACTTGTCACCAGATGATAAATCCTT
CAGCCGTGTCGTGCCCCGAGTGCCAGACTCCACCAGACGAACAGATGTGG
GTGCTGGTGCTTTGCGTCGTAGTGACTCTCCAGAAATTCCTTTCCAGGCT
GCTGCTGGCCCTTCTGATGGCTTAGATGCCTCCTCTCCAGGAAATAGCTT
TGTAGGGCTCCGTGTTGTAGCCAAGTGGTCATCCAATGGCTACTTTTACT
CTGGGAAAATCACACGAGATGTCGGAGCTGGGAAGTATAAATTGCTCTTT
GATGATGGGTACGAATGTGATGTGTTGGGCAAAGACATTCTGTTATGTGA
CCCCATCCCGCTGGACACTGAAGTGACGGCCCTCTCGGAGGATGAGTATT
TCAGTGCAGGAGTGGTGAAAGGACCACCGAAGAAGAAGCGCAAGGTCTGAggtacc

Amino Acid Sequence

MYPYDVPDYAPPLLPESTVSPQASTPISQSTPVFPPGSLPIPSQPQFSHD
IFIPSPSLEEQSNDGKKDGDMHSSSLTVECSKTSEIEPKNSPEDLGLSLT
GDSCKLMLSTSEYSQSPKMESLSSHRIDEDGENTQIEDTEPMSPVLNSKF
VPAENDSILMNPAQDGEVQLSQNDDKTKGDDTDTRDDISILATGCKGREE
TVAEDVCIDLTCDSGSQAVPSPATRSEALSSVLDQEEAMEIKEHHPEEGS
SGSEVEEIPETPCESQGEELKEENMESVPLHLSLTETQSQGLCLQKEMPK
KECSEAMEVETSVISIDSPQKLAILDQELEHKEQEAWEEATSEDSSVVIV
DVKEPSPRVDVSCEPLEGVEKCSDSQSWEDIAPEIEPCAENRLDTKEEKS
VEYEGDLKSGTAETEPVEQDSSQPSLPLVRADDPLRLDQELQQPQTQEKT
SNSLTEDSKMANAKQLSSDAEAQKLGKPSAHASQSFCESSSETPFHFTLP
KEGDIIPPLTGATPPLIGHLKLEPKRHSTPIGISNYPESTIATSDVMSES
MVETHDPILGSGKGDSGAAPDVDDKLCLRMKLVSPETEASEESLQFNLEK
PATGERKNGSTAVAESVASPQKTMSVLSCICEARQENEARSEDPPTTPIR
GNLLHFPSSQGEEEKEKLEGDHTIRQSQQPMKPISPVKDPVSPASQKMVI
QGPSSPQGEAMVTDVLEDQKEGRSTNKENPSKALIERPSQNNIGIQTMEC
SLRVPETVSAATQTIKNVCEQGTSTVDQNFGKQDATVQTERGSGEKPVSA
PGDDTESLHSQGEEEFDMPQPPHGHVLHRHMRTIREVRTLVTRVITDVYY
VDGTEVERKVTEETEEPIVECQECETEVSPSQTGGSSGDLGDISSFSSKA
SSLHRTSSGTSLSAMHSSGSSGKGAGPLRGKTSGTEPADFALPSSRGGPG
KLSPRKGVSQTGTPVCEEDGDAGLGIRQGGKAPVTPRGRGRRGRPPSRTT
GTRETAVPGPLGIEDISPNLSPDDKSFSRVVPRVPDSTRRTDVGAGALRR
SDSPEIPFQAAAGPSDGLDASSPGNSFVGLRVVAKWSSNGYFYSGKITRD
VGAGKYKLLFDDGYECDVLGKDILLCDPIPLDTEVTALSEDEYFSAGVVK
GPPKKKRKV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5304
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 124583 Daltons