cDNA Clone BRK

• Synonyms: PTK6
• Plasmid: pGEX6P-1-BRK
• Parent Plasmid: pGEX6P1
• Expressed: GST-BRK
• Genbank: NM_005975
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGTGTCCCGGGACCAGGCTCACCTGGGCCCCAAGTATGTGGGCCTCTGGGACTTCAAGTCCCGGACGGACGAGGAGCTGAGCTTCCGCGCGGGGGACGTCTTCCACGTGGCCAGGAAGGAGGAGCAGTGGTGGTGGGCCACGCTGCTGGACGAGGCGGGTGGGGCCGTGGCCCAGGGCTATGTGCCCCACAACTACCTGGCCGAGAGGGAGACGGTGGAGTCGGAACCGTGGTTCTTTGGCTGCATCTCCCGCTCGGAAGCTGTGCGTCGGCTGCAGGCCGAGGGCAACGCCACGGGC
GCCTTCCTGATCAGGGTCAGCGAGAAGCCGAGTGCCGACTACGTCCTGTC
GGTGCGGGACACGCAGGCTGTGCGGCACTACAAGATCTGGCGGCGTGCCG
GGGGCCGGCTGCACCTGAACGAGGCGGTGTCCTTCCTCAGCCTGCCCGAG
CTTGTGAACTACCACAGGGCCCAGAGCCTGTCCCACGGCCTGCGGCTGGC
CGCGCCCTGCCGGAAGCACGAGCCTGAGCCCCTGCCCCATTGGGATGACT
GGGAGAGGCCGAGGGAGGAGTTCACGCTCTGCAGGAAGCTGGGGTCCGGC
TACTTTGGGGAGGTCTTCGAGGGGCTCTGGAAAGACCGGGTCCAGGTGGC
CATTAAGGTGATTTCTCGAGACAACCTCCTGCACCAGCAGATGCTGCAGT
CGGAGATCCAGGCCATGAAGAAGCTGCGGCACAAACACATCCTGGCGCTG
TACGCCGTGGTGTCCGTGGGGGACCCCGTGTACATCATCACGGAGCTCAT
GGCCAAGGGCAGCCTGCTGGAGCTGCTCCGCGACTCTGATGAGAAAGTCC
TGCCCGTTTCGGAGCTGCTGGACATCGCCTGGCAGGTGGCTGAGGGCATG
TGTTACCTGGAGTCGCAGAATTACATCCACCGGGACCTGGCCGCCAGGAA
CATCCTCGTCGGGGAAAACACCCTCTGCAAAGTTGGGGACTTCGGGTTAG
CCAGGCTTATCAAGGAGGACGTCTACCTCTCCCATGACCACAATATCCCC
TACAAGTGGACGGCCCCTGAAGCGCTCTCCCGAGGCCATTACTCCACCAA
ATCCGACGTCTGGTCCTTTGGGATTCTCCTGCATGAGATGTTCAGCAGGG
GTCAGGTGCCCTACCCAGGCATGTCCAACCATGAGGCCTTCCTGAGGGTG
GACGCCGGCTACCGCATGCCCTGCCCTCTGGAGTGCCCGCCCAGCGTGCA
CAAGCTGATGCTGACATGCTGGTGCAGGGACCCCGAGCAGAGACCCTGCT
TCAAGGCCCTGCGGGAGAGGCTCTCCAGCTTCACCAGCTACGAGAACCCG
ACCTGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVSRDQAHLGPKYVGLWDF
KSRTDEELSFRAGDVFHVARKEEQWWWATLLDEAGGAVAQGYVPHNYLAE
RETVESEPWFFGCISRSEAVRRLQAEGNATGAFLIRVSEKPSADYVLSVR
DTQAVRHYKIWRRAGGRLHLNEAVSFLSLPELVNYHRAQSLSHGLRLAAP
CRKHEPEPLPHWDDWERPREEFTLCRKLGSGYFGEVFEGLWKDRVQVAIK
VISRDNLLHQQMLQSEIQAMKKLRHKHILALYAVVSVGDPVYIITELMAK
GSLLELLRDSDEKVLPVSELLDIAWQVAEGMCYLESQNYIHRDLAARNIL
VGENTLCKVGDFGLARLIKEDVYLSHDHNIPYKWTAPEALSRGHYSTKSD
VWSFGILLHEMFSRGQVPYPGMSNHEAFLRVDAGYRMPCPLECPPSVHKL
MLTCWCRDPEQRPCFKALRERLSSFTSYENPT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5577
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 78655 Daltons