cDNA Clone CK1 gamma

• Synonyms: CSNK1G1
• Plasmid: pEBG6P-1 CK1 gamma
• Parent Plasmid: pEBG6P
• Expressed: GST-CK1 gamma
• Genbank: NM_001011664
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGACCATCCTAGTAGGGAAAAGGATGAAAGACAACGGACAACTAAACCCATGGCACAAAGGAGTGCACACTGCTCTCGACCATCTGGCTCCTCATCGTCCTCTGGGGTTCTTATGGTGGGACCCAACTTCAGGGTTGGCAAGAAGATAGGATGTGGGAACTTCGGAGAGCTCAGATTAGGTAAAAATCTCTACACCAATGAATATGTAGCAATCAAACTGGAACCAATAAAATCACGTGCTCCACAGCTTCATTTAGAGTACAGATTTTATAAACAGCTTGGCAGTGCAGGTGAAGGT
CTCCCACAGGTGTATTACTTTGGACCATGTGGGAAATATAATGCCATGGT
GCTGGAGCTCCTTGGCCCTAGCTTGGAGGACTTGTTTGACCTCTGTGACC
GAACATTTACTTTGAAGACGGTGTTAATGATAGCCATCCAGCTGCTTTCT
CGAATGGAATACGTGCACTCAAAGAACCTCATTTACCGAGATGTCAAGCC
AGAGAACTTCCTGATTGGTCGACAAGGCAATAAGAAAGAGCATGTTATAC
ACATTATAGACTTTGGACTGGCCAAGGAATACATTGACCCCGAAACCAAA
AAACACATACCTTATAGGGAACACAAAAGTTTAACTGGAACTGCAAGATA
TATGTCTATCAACACGCATCTTGGCAAAGAGCAAAGCCGGAGAGATGATT
TGGAAGCCCTAGGCCACATGTTCATGTATTTCCTTCGAGGCAGCCTCCCC
TGGCAAGGACTCAAGGCTGACACATTAAAAGAGAGATATCAAAAAATTGG
TGACACCAAAAGGAATACTCCCATTGAAGCTCTCTGTGAGAACTTTCCAG
AGGAGATGGCAACCTACCTTCGATATGTCAGGCGACTGGACTTCTTTGAA
AAACCTGATTATGAGTATTTACGGACCCTCTTCACAGACCTCTTTGAAAA
GAAAGGCTACACCTTTGACTATGCCTATGATTGGGTTGGGAGACCTATTC
CTACTCCAGTAGGGTCAGTTCACGTAGATTCTGGTGCATCTGCAATAACT
CGAGAAAGCCACACACATAGGGATCGGCCATCACAACAGCAGCCTCTTCG
AAATCAGGTGGTTAGCTCAACCAATGGAGAGCTGAATGTTGATGATCCCA
CGGGAGCCCACTCCAATGCACCAATCACAGCTCATGCCGAGGTGGAGGTA
GTGGAGGAAGCTAAGTGCTGCTGTTTCTTTAAGAGGAAAAGGAAGAAGAC
TGCTCAGCGCCACAAGTGAGGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMDHPSREKDERQR
TTKPMAQRSAHCSRPSGSSSSSGVLMVGPNFRVGKKIGCGNFGELRLGKN
LYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLGSAGEGLPQVYYFGPCGKY
NAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLLSRMEYVHSKNLIYR
DVKPENFLIGRQGNKKEHVIHIIDFGLAKEYIDPETKKHIPYREHKSLTG
TARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERY
QKIGDTKRNTPIEALCENFPEEMATYLRYVRRLDFFEKPDYEYLRTLFTD
LFEKKGYTFDYAYDWVGRPIPTPVGSVHVDSGASAITRESHTHRDRPSQQ
QPLRNQVVSSTNGELNVDDPTGAHSNAPITAHAEVEVVEEAKCCCFFKRK
RKKTAQRHK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5581
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express CK1 gamma with N-terminal GST (contains preScission protease site); N.B. Sequence difference compared with NM_001011664: t717c (silent in H239).; ; Estimated MW of expressed protein : 75927 Daltons