cDNA Clone CHK1

• Synonyms: CHEK1
• Plasmid: pCMV5-FLAG CHK1
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-CHK1
• Genbank: BC004202
• Tag: FLAG
• Species: Human

Sequence of Insert

gaattcATGGCAGTGCCCTTTGTGGAAGACTGGGACTTGGTGCAAACCCTGGGAGAAGGTGCCTATGGAGAAGTTCAACTTGCTGTGAATAGAGTAACTGAAGAAGCAGTCGCAGTGAAGATTGTAGATATGAAGCGTGCCGTAGACTGTCCAGAAAATATTAAGAAAGAGATCTGTATCAATAAAATGCTAAATCATGAAAATGTAGTAAAATTCTATGGTCACAGGAGAGAAGGCAATATCCAATATTTATTTCTGGAGTACTGTAGTGGAGGAGAGCTTTTTGACAGAATAGAGCCAGACATA
GGCATGCCTGAACCAGATGCTCAGAGATTCTTCCATCAACTCATGGCAGG
GGTGGTTTATCTGCATGGTATTGGAATAACTCACAGGGATATTAAACCAG
AAAATCTTCTGTTGGATGAAAGGGATAACCTCAAAATCTCAGACTTTGGC
TTGGCAACAGTATTTCGGTATAATAATCGTGAGCGTTTGTTGAACAAGAT
GTGTGGTACTTTACCATATGTTGCTCCAGAACTTCTGAAGAGAAGAGAAT
TTCATGCgGAACCAGTTGATGTTTGGTCCTGTGGAATAGTACTTACTGCA
ATGCTCGCTGGAGAATTGCCATGGGACCAACCCAGTGACAGCTGTCAGGA
GTATTCTGACTGGAAAGAAAAAAAAACATACCTCAACCCTTGGAAAAAAA
TCGATTCTGCTCCTCTAGCTCTGCTGCATAAAATCTTAGTTGAGAATCCA
TCAGCAAGAATTACCATTCCAGACATCAAAAAAGATAGATGGTACAACAA
ACCCCTCAAGAAAGGGGCAAAAAGGCCCCGAGTCACTTCAGGTGGTGTGT
CAGAGTCTCCCAGTGGATTTTCTAAGCACATTCAATCCAATTTGGACTTC
TCTCCAGTAAACAGTGCTTCTAGTGAAGAAAATGTGAAGTACTCCAGTTC
TCAGCCAGAACCCCGCACAGGTCTTTCCTTATGGGATACCAGCCCCTCAT
ACATTGATAAATTGGTACAAGGGATCAGCTTTTCCCAGCCCACATGTCCT
GATCATATGCTTTTGAATAGTCAGTTACTTGGCACCCCAGGATCCTCACA
GAACCCCTGGCAGCGGTTGGTCAAAAGAATGACACGATTCTTTACCAAAT
TGGATGCAGACAAATCTTATCAATGCCTGAAAGAGACTTGTGAGAAGTTG
GGCTATCAATGGAAGAAAAGTTGTATGAATCAGGTTACTATATCAACAAC
TGATAGGAGAAACAATAAACTCATTTTCAAAGTGAATTTGTTAGAAATGG
ATGATAAAATATTGGTTGACTTCCGGCTTTCTAAGGGTGATGGATTGGAG
TTCAAGAGACACTTCCTGAAGATTAAAGGGAAGCTGATTGATATTGTGAG
CAGCCAGAAGGTTTGGCTTCCTGCCACATGAgcggccgc

Amino Acid Sequence

MDYKDDDDKGSPEFMAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVA
VKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEY
CSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENL
LLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHA
EPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDS
APLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSES
PSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYID
KLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDA
DKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDK
ILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKVWLPAT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5929
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence difference compared with BC004202 CDS: a558g (silent); ; Estimated MW of expressed protein : 56062 Daltons