cDNA Clone CMyc

• Plasmid: pETBlue2 6his cMyc
• Parent Plasmid: pETBlue2 original
• Expressed: 6His-cMyc
• Genbank: V00568
• Tag: 6His
• Species: Human

Sequence of Insert

ccatgggccatcaccatcaccatcaccccctcaacgttagcttcaccaac
aggaactatgacctcgactacgactcggtgcagccgtatttctactgcga
cgaggaggagaacttctaccagcagcagcagcagagcgagctgcagcccc
cggcgcccagcgaggatatctggaagaaattcgagctgctgcccaccccg
cccctgtcccctagccgccgctccgggctctgctcgccctcctacgttgc
ggtcacacccttctcccttcggggagacaacgacggcggtggcgggagct
tctccacggccgaccagctggagatggtgaccgagctgctgggaggagac
atggtgaaccagagtttcatctgcgacccggacgacgagaccttcatcaa
aaacatcatcatccaggactgtatgtggagcggcttctcggccgccgcca
agctcgtctcagagaagctggcctcctaccaggctgcgcgcaaagacagc
ggcagcccgaaccccgcccgcggccacagcgtctgctccacctccagctt
gtacctgcaggatctgagcgccgccgcctcagagtgcatcgacccctcgg
tggtcttcccctaccctctcaacgacagcagctcgcccaagtcctgcgcc
tcgcaagactccagcgccttctctccgtcctcggattctctgctctcctc
gacggagtcctccccgcagggcagccccgagcccctggtgctccatgagg
agacaccgcccaccaccagcagcgactctgaggaggaacaagaagatgag
gaagaaatcgatgttgtttctgtggaaaagaggcaggctcctggcaaaag
gtcagagtctggatcaccttctgctggaggccacagcaaacctcctcaca
gcccactggtcctcaagaggtgccacgtctccacacatcagcacaactac
gcagcgcctccctccactcggaaggactatcctgctgccaagagggtcaa
gttggacagtgtcagagtcctgagacagatcagcaacaaccgaaaatgca
ccagccccaggtcctcggacaccgaggagaatgtcaagaggcgaacacac
aacgtcttggagcgccagaggaggaacgagctaaaacggagcttttttgc
cctgcgtgaccagatcccggagttggaaaacaatgaaaaggcccccaagg
tagttatccttaaaaaagccacagcatacatcctgtccgtccaagcagag
gagcaaaagctcatttctgaagaggacttgttgcggaaacgacgagaaca
gttgaaacacaaacttgaacagctacggaactcttgtgcgtaagaattc

Amino Acid Sequence

MGHHHHHHPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPP
APSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSF
STADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAK
LVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSV
VFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEE
TPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHS
PLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCT
SPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKV
VILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1059
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express N-terminal 6his fusion; Change to V00568 ORF sequence: ; g672c (silent in L224)