Plasmid
pGEX6P-1-HA-BRSK1
Parent Plasmid
pGEX6P1
Expressed
GST-HA-BRSK1
Genbank
NM_032430
Tag
GST
HA
Species
Human
Sequence of Insert
View Sequence
ggatccTACCCATACGATGTGCCAGATTACGCCTCGTCCGGGGCCAAGGAGGGAGGTGGGGGCTCTCCCGCCTACCACCTCCCCCACCCCCACCCCCACCCACCCCAGCACGCCCAATATGTGGGCCCCTATCGGCTGGAGAAGACGCTGGGCAAAGGACAGACAGGGCTGGTTAAACTCGGGGTCCACTGCATCACGGGTCAGAAGGTCGCCATCAAGATCGTGAACCGGGAGAAGCTGTCGGAGTCGGTGCTGATGAAGGTGGAGCGGGAGATCGCCATCCTGAAGCTCATCGAACACCCACATGTCCTCAAGCTCCACGACGTCTACGAGAACAAGAAATATTTGTACCTGGTTCTGGAGCACGTCTCGGGGGGTGAGCTATTCGACTACCTGGTAAAGAAGGGGAGACTGACGCCCAAGGAGGCCCGAAAGTTCTTCCGCCAGATTGTGTCTGCGCTGGACTTCTGCCACAGCTACTCCATCTGCCACAGAGACCTAAAGCCCGAGAACCTGCTTTTGGATGAGAAAAACAACATCCGCATTGCAGACTTCGGCATGGCGTCCCTGCAGGTGGGGGACAGCCTCCTGGAGACCAGCTGCGGGTCCCCCCATTATGCGTGTCCAGAGGTGATTAAGGGGGAAAAATATGATGGCCGCCGGGCAGACATGTGGAGCTGTGGAGTCATCCTCTTCGCCCTGCTCGTGGGGGCTCTGCCCTTTGATGACGACAACCTCCGCCAGCTGCTGGAGAAGGTGAAACGGGGCGTCTTCCACATGCCCCACTTCATTCCTCCAGATTGCCAGAGCCTCCTGAGGGGAATGATCGAAGTGGAGCCCGAAAAAAGGCTCAGTCTGGAGCAAATTCAGAAACATCCTTGGTACCTAGGCGGGAAACACGAGCCAGACCCGTGCCTGGAGCCAGCCCCTGGCCGCCGGGTAGCCATGCGGAGCCTGCCATCCAACGGAGAGCTGGACCCCGACGTCCTAGAGAGCATGGCATCACTGGGCTGCTTCAGGGACCGCGAGAGGCTGCATCGCGAGCTGCGCAGTGAGGAGGAGAACCAAGAAAAGATGATATATTATCTGCTTTTGGATCGGAAGGAGCGGTATCCCAGCTGTGAGGACCAGGACCTGCCTCCCCGGAATGATGTTGACCCCCCCCGGAAGCGTGTGGATTCTCCCATGCTGAGCCGTCACGGGAAGCGGCGACCAGAGCGGAAGTCCATGGAAGTCCTGAGCATCACCGATGCCGGGGGTGGTGGCTCCCCTGTACCCACCCGACGGGCCTTGGAGATGGCCCAGCACAGCCAGAGATCCCGTAGCGTCAGTGGAGCCTCCACGGGTCTGTCCTCCAGCCCTCTAAGCAGCCCAAGGAGTCCGGTCTTTTCCTTTTCACCGGAGCCGGGGGCTGGAGATGAGGCTCGAGGCGGGGGCTCCCCGACTTCCAAAACGCAGACGCTGCCTTCTCGGGGCCCCAGGGGTGGGGGCGCCGGGGAGCAGCCCCCGCCCCCCAGTGCCCGCTCCACACCCCTGCCCGGCCCCCCAGGCTCCCCGCGCTCCTCTGGCGGGACCCCCTTGCACTCGCCTCTGCACACGCCCCGGGCCAGTCCCACCGGGACCCCGGGGACAACACCACCCCCCAGCCCCGGCGGTGGCGTCGGGGGAGCCGCCTGGAGGAGTCGTCTCAACTCCATCCGCAACAGCTTCCTGGGCTCCCCTCGCTTTCACCGGCGCAAGATGCAGGTCCCTACCGCTGAGGAGATGTCCAGCTTGACGCCAGAGTCCTCCCCGGAGCTGGCAAAACGCTCCTGGTTCGGGAACTTCATCTCCTTGGACAAAGAAGAACAAATATTCCTCGTGCTAAAGGACAAACCTCTCAGCAGCATCAAAGCAGACATCGTCCATGCCTTTCTGTCGATCCCCAGCCTGAGTCACAGTGTGCTGTCACAGACCAGCTTCAGGGCCGAGTACAAGGCCAGTGGCGGCCCCTCCGTCTTCCAAAAGCCCGTCCGCTTCCAGGTGGACATCAGCTCCTCTGAGGGTCCAGAGCCCTCCCCGCGACGGGACGGCAGCGGAGGTGGTGGCATCTACTCCGTCACCTTCACTCTCATCTCGGGTCCCAGCCGTCGGTTCAAGCGAGTGGTGGAGACCATCCAGGCACAGCTCCTGAGCACTCATGACCAGCCCTCCGTGCAGGCCCTGGCAGACGAGAAGAACGGGGCCCAGACCCGGCCTGCTGGTGCCCCACCCCGAAGCCTGCAGCCCCCACCCGGCCGCCCAGACCCAGAGCTGAGCAGCTCTCCCCGCCGAGGCCCCCCCAAGGACAAGAAGCTCCTGGCCACCAACGGGACCCCTCTGCCCTGAAAGCCgaattc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSYPYDVPDYASSGAKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGGGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRGPPKDKKLLATNGTPLP*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSYPYDVPDYASSGAKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGGGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRGPPKDKKLLATNGTPLP*
Vector Type
Bacterial
Antibiotic
Amp
DU Number
DU1170
Unit Source
Location
PPU
MTA
Notes
Bacterial construct to express BRSK1 with N-terminal GST and HA tags.
Price
£125.00
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