cDNA Clone CDC45 (yeast)

• Plasmid: pRS416 PRO CDC45 y 3′UTR K576/T
• Parent Plasmid: pRS416
• Genbank: NP_013204
• Species: Yeast

Sequence of Insert

GGATCCCATCACAAGGGTTATTTAAAACTTGATCTTCATATGATTGTTGCTGTTGCTGTTGCTGTTGCTGATGATGACCGAAATTGAAGAAGGCATTCACTCCAGTGCTGCCAAAAATAAACAATGCAAAGATGAGAACGATACTCCAGCGACTTTGACTCATACTACAGTAAAATTTGGTCGTTTTTTGCCTGGCAAACCATGCCTATTTTTCGTCATTAAAGGTCTTCTGAGCTTTCATCAAAATTATCTCTATAATTATAAAGGACAGCTCAGAGCCACACCCAAACATCTGCAAAGGTAAAGGCCATCTTTCATCTCACATTGCCTAACAAGAAAGTCACATTGCAATTGTTTCGAGAAGAAAACATTAATTCAATTACGTGGTTCCCATGCGTCTGTGTTAAGCGTCAATTTATCATTTGACGAACTCGTTGCAACCACAAACAAAGCAAGGGCCGCATTTATCAAAGGAAAACCAACCAAAAGCATAAATAAACGAGATTTTTATTATGAAAAAGCCATGCGAATCCTACTAGAATAAATTCAAAGTGAAGACATTTAGTCAATTCGATCTTCTCTCTCTTGCTTTAACTAGTCGTAAAACCTAATAAGTTTCATTCACGACGCGTATTTTTTTTTCAACATATCATCTAACGCGTTTTAAGAAATTTCAGTAAATAATGAAGCTGTAGTAATGCCAACAATCCATTGTGGCTACTTTATACAACGCACTATAGAGCAATAAAAGTGTAGCAATAAACACTAGAGAGAAGGCACATAATAACAAGAATATACTCTCGCACATGTATTATGGAATCAGCCAGTTTAGCGAAGCCTACAACAAAATCTTAAGGAATTCATCGTCTCATTCATCATGTCAATTGGTCATTTTCGTTTCTTGTCTTAACATCGATGCGCTGTGTGCGACGAAGATGTTGTCACTATTATTCAAAAAACAATTGGTACAATCTCAAATAGTACCGATATTTGGGTACTCTGAATTACGACGCCACTATTCTCAATTGGATGACAATATAAATAGTCTGCTATTAGTAGGGTTTGGAGGCGTTATTGATCTGGAGGCTTTCTTAGAAATCGATCCCCAAGAGTATGTTATCGATACAGATGAAAAGTCGGGGGAACAAAGTTTCAGGAGAGACATCTACGTGTTGGATGCTCATAGACCGTGGAATCTCGACAATATATTTGGATCACAAATCATCCAATGTTTTGACGACGGTACGGTGGATGACACATTAGGCGAACAAAAAGAAGCATACTATAAATTGCTTGAACTGGACGAGGAGAGTGGTGACGATGAGCTCTCAGGTGACGAGAACGATAATAATGGGGGTGACGATGAGGCAACTGATGCCGATGAAGTCACAGACGAGGACGAAGAAGACGAAGATGAGACAATATCTAATAAAAGAGGTAATTCTTCAATAGGACCGAATGATCTCTCAAAAAGAAAGCAACGAAAGAAGCAAATCCATGAATATGAAGGTGTTTTGGAGGAATACTATTCTCAAGGCACAACAGTGGTTAACTCGATATCTGCACAAATCTATTCATTATTGTCTGCTATCGGAGAAACAAATCTTTCAAACCTGTGGTTGAATATACTTGGTACGACCTCGTTAGATATAGCATATGCTCAAGTTTACAATCGATTATACCCCTTATTGCAAGATGAAGTGAAGCGTCTCACACCAAGCAGTAGAAACTCAGTAAAGACCCCTGATACATTGACGTTAAACATCCAACCGGATTACTACCTTTTCTTACTGAGGCATTCTTCATTGTACGACAGTTTTTATTATTCTAACTATGTCAATGCCAAACTATCCCTATGGAATGAAAATGGGAAAAAGAGATTGCATAAGATGTTTGCTAGAATGGGTATACCATTAAGTACTGCACAAGAAACATGGTTATACATGGATCATTCTATTAAGAGAGAACTTGGGATAATATTTGACAAAAATTTGGATCGTTATGGGTTGCAAGATATAATTAGAGATGGGTTTGTTAGAACTCTTGGATATCGTGGGTCCATAAGTGCCAGTGAATTTGTCGAAGCACTAACAGCTCTTTTGGAAGTAGGTAATTCAACTGATAAGGATAGTGTGAAAATAAATAATGACAATAATGACGATACAGATGGAGAGGAAGAAGAAGATAACAGCGCTCAAAAATTGACGAATTTGAGAAAAAGATGGGTTTCGAATTTTTGGCTCAGTTGGGATGCTCTAGATGACAGAAAGGTGGAACTATTAAACCGTGGCATTCAACTAGCACAAGACTTACAGAGAGCAATTTTTAATACCGGGGTTGCTATATTGGAGAAGAAATTAATCAAGCATTTAAGAATTTATAGATTATGCGTCTTACAAGACGGACCCGATTTAGACTTGTACAGAAACCCATTGACGTTATTAAGATTAGGAAATTGGCTCATAGAATGCTGTGCGGAATCTGAAGACAAGCAATTGTTGCCCATGGTGCTTGCCAGCATAGATGAAAATACGGACACTTACTTGGTTGCTGGGTTAACACCTAGGTATCCTCGCGGACTAGACACGATACACACAAAAAAACCTATTTTGAATAATTTCAGCATGGCGTTTCAACAAATAACTGCAGAAACGGATGCTAAAGTGAGAATAGATAATTTTGAAAGTTCCATAATTGAAATACGTCGTGAAGATCTTTCACCATTCCTGGAGAAGCTGACCTTGAGTGGATTGTTATAATCAAATTTATATTATTTAGTCGTACATATATACCAGCATATGAATATAAAATCTAAGCTTTTTCAAGCTTTTAAGTCCAACTTTTCTATAATAATACCATATTTTGTGCGCGGCTCTTGACAAAACAAAAAAAAAAACCTAAATAAAAGGAAATTAAGAACAGGGAACTAGGTTTGGAAGGACAAATTCAGGAAGGAAAGGCGGCCGC

Amino Acid Sequence

MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVPIFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDIYVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDENDNNGGDDEATDADEVTDEDEEDEDETISNKRGNSSIGPNDLSKRKQRKKQIHEYEGVLEEYYSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYPLLQDEVKRLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNENGKKRLHKMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTLGYRGSISASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRWVSNFWLSWDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDGPDLDLYRNPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLDTIHTKKPILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU19576
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression construct to express CDC45.