cDNA Clone CRHSP24

• Plasmid: pCMV5 STREP P 6His CRHSP24
• Parent Plasmid: pCMV5 strep 3C 6His
• Expressed: pCMV5 STREP P 6His CRHSP24
• Genbank: NM_014316
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCTCATCTGA
GCCTCCCCCACCACCACAGCCCCCCACCCATCAAGCTTCAGTCGGGCTGC
TGGACACCCCTCGGAGCCGTGAGCGCTCACCATCCCCTCTGCGGGGCAAC
GTGGTCCCAAGCCCACTGCCCACTCGCCGGACGAGGACCTTCTCGGCGAC
GGTGCGGGCTTCACAGGTCCCCGTCTACAAAGGAGTCTGCAAATGCTTCT
GCCGGTCCAAGGGCCATGGCTTCATTACCCCAGCTGATGGCGGCCCCGAC
ATCTTCCTGCACATCTCTGATGTGGAAGGGGAGTATGTCCCAGTGGAAGG
CGACGAGGTCACCTATAAAATGTGCTCCATCCCACCCAAGAATGAGAAGC
TGCAGGCCGTGGAGGTCGTCATCACTCACCTGGCACCAGGCACCAAGCAT
GAGACCTGGTCTGGACATGTCATCAGCTCCTAGGTCGAC

Amino Acid Sequence

MASSLRQILDSQKMEWRSNAGGSELALEVLFQGPWHHHHHHGSPEFATMY
PYDVPDYASSEPPPPPQPPTHQASVGLLDTPRSRERSPSPLRGNVVPSPL
PTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHIS
DVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGH
VISS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3675
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal STREP/6His/HA tag for mammalian expression (Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7480-5)