cDNA Clone (mu) MYCBP2

• Plasmid: pSc-B-(mu)MYCBP2 nt 4155 - 6292
• Parent Plasmid: pSC-B
• Expressed: Mouse Mycbp2
• Genbank: NM_207215.2 → NP_997098.2
• Species: Mouse

Sequence of Insert

GGTACCGATGTAAATGCTGGTCAGATACCTCAACTGTTATACAGACTCCC
GACCAGTGACGGCAGTACCTCAAAAGGCAAGCAGCAAACGAGTGAGCCTG
TCCACATCTTAAAGAGGTCTTTTGCAAGAACTGTCTCCGTGGAGTGCTTC
GAGTCGCTGCTGAGCATTCTTCACTGGAGCTGGACCACCTTAGTCTTAGG
AGTGGAAGAGCTCAGGGGGCTGAAAGGGTTCCAGTTCACCGCTACACTCC
TTGACTTAGAGCGGCTGCGCTTCGTGGGCACCTGTTGTCTGCGGTTACTG
CGCGTCTACACCTGTGAGATATACCCAGTATCAGCTACAGGAAAAGCAGT
TGTTGAAGAAACTAGCAAATTAGCTGAGTGTATTGGAAAAACAAGAACTT
TGTTAAGAAAAATCTTATCGGAAGGAGTTGATCATTGCATGGTGAAGTTG
GATAATGACCCTCAGGGATACCTCAGCCAGCCCCTGAGGCTACTAGAAGC
TGTGCTTCAGGAGTGCCACAACACCTTCACCGCCTGCTTTCACTCCTTCT
ACCCAACGCCGGCACTACAGTGGGCATGCCTCTGTGATCTGCTCAATTGC
CTGGATCAGGATATCCAGGAAGCAAACTTTAAGACGTCAAGTAGCCGGCT
CCTTGCAGCTGTCATGTCAGCTCTGTGTCACACTTCTGTGAAGCTGACTT
CTCTCTTCCCTATTGCATACGATGGAGAAGTGTTGCTGCGATCGATTGTT
AAGCAAGTCAGCACGGAGAACGACTCAACACTAGTTCATCGTTTTCCACT
TTTAGTGGGACATATGGAAAAGCTCAGCCAGAGTGAAGAGAACATCTCCG
GCATGACGAGCTTCCGGGAAGTGCTGGAGAAGATGCTGGTCATTGTTGTG
CTTCCAGTGAGAAACAGCCTTCGGAGGGAGAGTGAGCTCTTCTCCTCTCA
CCTGGTCTCCAACACCTGCGGACTGCTGGCCAGCATTGTCAGTGAGCTGA
CTGCATCAGCCCTCGGGTCGGAGGTTGATGGACTTAATTCTCTTCACTCT
GTCAAAGCCAGTGCTAACCGATTCACAAAAACAAGTCAGGGAAGAAGTTG
GAACACTGGAAATGGGTCCCCTGATGCAATCTGTTTTGCAGTCGACAAAC
CTGGAATTGTGGTTGTTGGTTTCGCTGTCTATGGAGGAGGCGGCATCCAT
GAGTATGAGCTGGAGGTGTTGGTTGATGATAGTGAACATGCAGGAGATTC
AACTCATTCTCATAGATGGACGTCTCTAGAATTAGTTAAAGGAACTTATA
CAACAGACGATTCACCCAGTGACATAGCTGAAATCAGACTTGACAAAGTT
GTTCCCTTAAAGGAAAATGTTAAGTATGCTGTGCGCTTGAGGAACTATGG
CAGTCGCACAGCCAATGGAGATGGAGGGATGACCACTGTTCAGTGCCCTG
ATGGAGTGACCTTTACCTTCAGCACATGCAGCTTGAGCAGTAATGGCACA
AACCAAACCAGAGGACAGATTCCCCAGATACTATACTATAGGAGTGAATT
TGATGGAGATTTACAATCCCAACTTCTAAGTAAAGCTAATGAAGAAGATA
AAAACTGTAGCAGAGCACTCTCTGTGGTAAGCACTGTTGTTCGAGCTGCG
AAGGACCTGTTGCACAGAGCTCTTGCTGTGGATGCTGATGACATTCCAGA
ACTGCTTAGCTCTTCCAGTCTGTTTTCCATGCTGCTTCCCCTTATTATAG
CCTACATAGGACCAGTAGCCGCTGCTATTCCCAAGGTTGCTGTAGAAGTT
TTTGGCCTTGTCCAGCAATTGCTTCCCTCAGTTGCCATTTTGAATCAGAA
GTATGCACCCCCTGCGTTCAATCCCAATCAGTCAACTGATAGTACGACAG
GCAACCAGCCCGAGCAAGGCCTCTCAGCTTGTACCACTTCCAATCACTAT
GCTGTCATTGAGAGTGAGCACCCATACAAGCCTGCCTGTGTGATGCACTA
CAAGGTGACATTCCCAGAATGTGTTAGGTGGATGACAATCGAATTTGACC
CTCAGTGTGGTACTGCACAGTCAGAAGACGTCATCCGTTTGCTTATTCCT
GTCAGAACCATTCAGAACTCTGGATATGGAGCAAAACTGACATCTGTTCA
TGAAAACCTTAATTCATGGGTAGAATTAAAGAAATATTCAGGATCC

Amino Acid Sequence

GTDVNAGQIPQLLYRLPTSDGSTSKGKQQTSEPVHILKRSFARTVSVECF
ESLLSILHWSWTTLVLGVEELRGLKGFQFTATLLDLERLRFVGTCCLRLL
RVYTCEIYPVSATGKAVVEETSKLAECIGKTRTLLRKILSEGVDHCMVKL
DNDPQGYLSQPLRLLEAVLQECHNTFTACFHSFYPTPALQWACLCDLLNC
LDQDIQEANFKTSSSRLLAAVMSALCHTSVKLTSLFPIAYDGEVLLRSIV
KQVSTENDSTLVHRFPLLVGHMEKLSQSEENISGMTSFREVLEKMLVIVV
LPVRNSLRRESELFSSHLVSNTCGLLASIVSELTASALGSEVDGLNSLHS
VKASANRFTKTSQGRSWNTGNGSPDAICFAVDKPGIVVVGFAVYGGGGIH
EYELEVLVDDSEHAGDSTHSHRWTSLELVKGTYTTDDSPSDIAEIRLDKV
VPLKENVKYAVRLRNYGSRTANGDGGMTTVQCPDGVTFTFSTCSLSSNGT
NQTRGQIPQILYYRSEFDGDLQSQLLSKANEEDKNCSRALSVVSTVVRAA
KDLLHRALAVDADDIPELLSSSSLFSMLLPLIIAYIGPVAAAIPKVAVEV
FGLVQQLLPSVAILNQKYAPPAFNPNQSTDSTTGNQPEQGLSACTTSNHY
AVIESEHPYKPACVMHYKVTFPECVRWMTIEFDPQCGTAQSEDVIRLLIP
VRTIQNSGYGAKLTSVHENLNSWVELKKYSGS

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU65484
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: From native Kpn1 site at nt 4155 to native BamHI site at nt6292 ; Comprises GLY1371- Ser2099 of full-length protein.; Clone harbours a 9bp insertion at nt 4720 (relative to full-length NCBI numbering) giving a 3 residue insertion (Asp-Ile-Gln) between Q1573 and E1574
• Low Temp: No