cDNA Clone (mu) MYCBP2

• Plasmid: pSc-B-(mu)MYCBP2 nt6292 - 10,107
• Parent Plasmid: pSC-B
• Expressed: Mouse Mycbp2
• Genbank: NM_207215.2 → NP_997098.2
• Species: Mouse

Sequence of Insert

GGATCCTCTGGATGGCCTACTATGGTTTTAGTGTTGCCAGGAAATGAAGC
CCTTTTCTCACTGGAAACTGCTTCAGATTATGTGAAAGATGACAAAGCCT
CCTTCTATGGGTTCAAGTGTTTTGCAATTGGATATGAATTTAGCCCTGGA
CCTGATGAGGGAGTTATTCAGCTGGAAAAAGAATTAGCCAATCTTGGTGG
GGTTTGTGCCGCAGCCCTGATGAAGAAGGATCTCGCACTGCCTGTTGGTA
ACGAGTTGGAGGAAGATCTTGAAATTCTTGAAGAGGCTGCATTGCAGGTG
TGCAAAACTCACTCTGGCATTCTTGGAAAGGGTTTGGCTCTTTCTCATTC
ACCAACTATCTTAGAGGCACTTGAAGGAAACTTGCCGCTTCAAATCCAAA
GCAATGAGCAGTCCTTTCTGGATGATTTCATTGCTTGTGTCCCAGGGTCA
AGTGGTGGAAGGCTTGCAAGATGGCTTCAGCCAGATTCCTATGCTGACCC
TCAGAAAACTTCGTTGATTCTGAATAAGGATGATATTCGCTGTGGCTGGC
CCACCACCATAACTGTTCAAACAAAAGACCAATATGGCGATGTGGTTCAT
GTTCCCAACATGAAGGTGGAAGTGAAAGCTGTACCTGTTTCTCAGAAAAA
AACATCTTTGCAGCAAGATCAAGGAAAGAAATGTCAAAGGATTCCTGGCA
GCCCCTCAGCAGCAGCTTCTAGCGCCGACATGACTTTTGGTGGGTTGGCA
TCCCCAAAGCTGGACGTCTCGTATGAGCCAATGATCGTCAAGGAGGCGCG
GTATATTGCCATAACAATGATGAAGGTATATGAAAACTACTCATTTGAAG
AACTGCGTTTTGCATCACCAACTCCGAAAAGGCCCAGTGAAAATATGCTT
ATTCGTGTCAATAACGATGGGACCTACTGTGCAAACTGGACTCCAGGGGC
TATCGGGCTCTACACTGTTCATGTGACCATTGATGGCATTGAAATAGATG
CTGGCCTTGAAGTAAAAGTCAAAGATCCACCAAAGGGGATGATCCCCCCA
GGGACTCAGCTAGTCAAGCCGAAGGCAGACCCCCAGCCAAATAAGATTCG
AAAATTTGTGGCCAAGGACAGTGCTGGTCTCCGCATCCGGAGCCACCCTT
CCCTTCAGAGTGAACAGATTGGAATAGTGAGAGTCAATGGAACTATCACT
TTTATTGATGAGATCCACAATGATGATGGCGTGTGGTTGAGACTGAATGA
GGAGACAATAAAGAAGTATGTTCCTAACATGAACGGTTACACTGAAGCCT
GGTGCCTCTCTTTTAACCAGCATCTTGGCAAGAGCCTTCTGGTCCCTGTT
GACGAACCTAAAACTAATACTGATGACTTTTTCAAAGACATGAACTCCTG
CGGCCCACAGGAAGCAACAATGCAAGAACGAGATCACCCGTTCTTGAGAG
GTGGGCCAGGCATGTACAAGGTAGTGAAGACGGGGCCTTCAGGTCACAAC
ATCAGAAGCTGCCCTAACCTTAGAGGTATCCCAATTGGAATGTTAGTTCT
GGGAAACAAAGTCAAAGCAGTGGGAGAGGTAACCAATTCTGAAGGTGCAT
GGGTGCAGCTGGATAAGAACAGCATGGTAGAATTCTGTGAGAGTGATGAA
GGAGAGGCATGGTCCTTAGCTAGAGACAGAGGCGGAAACCAGTACCTACG
GCATGAAGATGAACAAGTTCTGCTGGATCAGAATTCTCAACCTCCTCCTC
CAAGCCCTTTCTCAGTACAAGCTTTCAATAAAGGGGCAAGTTGCAGTGCC
CAAGGATTTGACTATGGCCTGGGAAATAACAAAGGTGACAGAGGAACTAT
CTCAACATCTTCAAGACCAGTGTCCACATCAGGGAAGTCAGAGCTGCCCT
CTAAGCACAGCAGGTCAGTTAAACCCGACGGGCATGTGAGCCGGACTCCT
GCTGACCAGAAGAAGCCACGGGGGACAGAAGGCTTATCTGCTAGTGAATC
CCTCATGTTAAAATCTGATGCTGCAAAGTTGAGGTCAGACTCCCATAGTA
GGTCCCTGTCCCCTAACCATAACACTCTGCAGACACTGAAGTCTGATGGG
AGGACGTCTTCTGGCTTCAGGGCCGAATCCCCAGGACCAGGCTCGAGGTC
ATCCTCCCCTAAGCCCAAGCCTCTGCCGACTCCCAGGTCTAGCCCATCTG
GTGCAAGCTCTCCACGCTCCTCCTCACCACAGGATAAAAATCTACCTCAG
AAAAGCACAGCTCCTGCTAAGACAAAACTTGACCCACCTCGGGAACGTTC
CAAGTCAGACTCATATACACTTGATCCAGATACCCTCCGTAAGAAGAAAA
TGCCCCTCACAGAACCATTAAGGGGGCGGTCCACATCACCAAAACCAAAA
CCAGTACCAAAGGATCCTAAAGACTCCCCAGGGTCTGAAAACAGAGCCCC
TTCTCCCCACGTAGTGCAGGAAAACCTCCACAGTGAGGTAGTTGAAGTCT
GTACTTCAAGTACTTTAAAAACAAATGGTGTCACGGACAGCACCTGCGAT
GACAGCGGTGACTTGAAGAGTGTGGATGAAGGTTCAAATAAAGTGCATTT
CAGCATAGGCAAAGCACCGCTGAAAGATGAACAGGAAATGAGAGCATCAC
CCAAAATAAGTCGGAAGTGTGCTAACAGACACACCAGGCCCAAAAAAGAA
AAGTCTAACTTCCTTTTCAAAGGAGATGGAACCAAATCTTTAGAACCAGC
CAAGCAAGCCATGTCCCCGTCTGTGGCTGAGcGTGCCAGAGCCGTCTTTG
CCTCTTTTTTGTGGCATGAAGGCATAGTACATGATGCAATGGCTTGTTCT
TCCTTCCTGAAATTTAATCCTGACCTTTCCAAAGAACATGCTCCTATAAG
AAGTAGTTTGAATAGCCAACCACCCACAGAGGAAAAGGAAATCAAGTTAA
AAAATAGACATTCATTGGAAATATCGTCTGCTCTGAATATGTTTAACATT
GCACCTCATGGCCCAGATATATCCAAGATGGGTAGTATCAACAAAAATAA
GGTCTTGTCCATGCTTAAGGAACCGCCTCTCCATGAAAAATGTGAGGACG
GGAAATCGGAAGCCACTTTTGAAATGTCCATGCATCACACAATGAAGTCG
AAATCTCCACTTCCTTTAACATTACAACATTTAGTGGCTTTCTGGGAAGA
TATCTCTTTGGCTACAATCAAAGCTGCTTCCCAGAATATGATTTTTCCAA
GTCCTGGTTCCTGTGCAGTCCTTAAAAAGAAAGAATGTGAAAAAGAGAAT
AAAAAAACAAAAAAGGAAAAAAAGAAAAAAGAAAAGACAGAAATTCGGCC
AAGGGGCAATTTGTTTGGTGAAATGGCCCAGCTGGCTGTAGGAGGGCCAG
AGAAAGATACCATCTGTGAGCTGTGTGGAGAATCCCATCCGTACCCAGTG
ACATATCACATGAGACAAGCTCACCCAGGTTGTGGCCGATATGCTGGTGG
ACAAGGATACAATAGCATTGGGCATTTTTGTGGAGGATGGGCTGGTAACT
GTGGTGATGGGGGAATGGGAGGAAGCACTTGGTACC

Amino Acid Sequence

GSSGWPTMVLVLPGNEALFSLETASDYVKDDKASFYGFKCFAIGYEFSPG
PDEGVIQLEKELANLGGVCAAALMKKDLALPVGNELEEDLEILEEAALQV
CKTHSGILGKGLALSHSPTILEALEGNLPLQIQSNEQSFLDDFIACVPGS
SGGRLARWLQPDSYADPQKTSLILNKDDIRCGWPTTITVQTKDQYGDVVH
VPNMKVEVKAVPVSQKKTSLQQDQGKKCQRIPGSPSAAASSADMTFGGLA
SPKLDVSYEPMIVKEARYIAITMMKVYENYSFEELRFASPTPKRPSENML
IRVNNDGTYCANWTPGAIGLYTVHVTIDGIEIDAGLEVKVKDPPKGMIPP
GTQLVKPKADPQPNKIRKFVAKDSAGLRIRSHPSLQSEQIGIVRVNGTIT
FIDEIHNDDGVWLRLNEETIKKYVPNMNGYTEAWCLSFNQHLGKSLLVPV
DEPKTNTDDFFKDMNSCGPQEATMQERDHPFLRGGPGMYKVVKTGPSGHN
IRSCPNLRGIPIGMLVLGNKVKAVGEVTNSEGAWVQLDKNSMVEFCESDE
GEAWSLARDRGGNQYLRHEDEQVLLDQNSQPPPPSPFSVQAFNKGASCSA
QGFDYGLGNNKGDRGTISTSSRPVSTSGKSELPSKHSRSVKPDGHVSRTP
ADQKKPRGTEGLSASESLMLKSDAAKLRSDSHSRSLSPNHNTLQTLKSDG
RTSSGFRAESPGPGSRSSSPKPKPLPTPRSSPSGASSPRSSSPQDKNLPQ
KSTAPAKTKLDPPRERSKSDSYTLDPDTLRKKKMPLTEPLRGRSTSPKPK
PVPKDPKDSPGSENRAPSPHVVQENLHSEVVEVCTSSTLKTNGVTDSTCD
DSGDLKSVDEGSNKVHFSIGKAPLKDEQEMRASPKISRKCANRHTRPKKE
KSNFLFKGDGTKSLEPAKQAMSPSVAERARAVFASFLWHEGIVHDAMACS
SFLKFNPDLSKEHAPIRSSLNSQPPTEEKEIKLKNRHSLEISSALNMFNI
APHGPDISKMGSINKNKVLSMLKEPPLHEKCEDGKSEATFEMSMHHTMKS
KSPLPLTLQHLVAFWEDISLATIKAASQNMIFPSPGSCAVLKKKECEKEN
KKTKKEKKKKEKTEIRPRGNLFGEMAQLAVGGPEKDTICELCGESHPYPV
TYHMRQAHPGCGRYAGGQGYNSIGHFCGGWAGNCGDGGMGGSTWY

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU65485
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: From native BamHI site at nt6292 to native Kpn1 site at nt10,107.; Comprises Gly2098-Tyr 3369 of full-length protein with the following changes:; Deletion spanning N2549-Q2573, Deletion spanning D2735-S2786 and a single mutation at C3102>R (TGT > CGT)
• Low Temp: No