cDNA Clone (mu)RNF146

• Plasmid: pGEX-TEV-(mu)RNF146 Ala30-Arg183
• Parent Plasmid: pGEX-TEV
• Expressed: GST-RNF146 (RING-WWE)
• Genbank: NM_001110196.1
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCGCACCTTCTCTGACAGTTCCTGAATGTGCCATTTGTCTACAAACATGTGTTCACCCTGTCAGTCTGCCCTGTAAGCATGTTTTCTGTTATCTGTGTGTAAAGGGTGCTTCATGGCTTGGGAAGCGATGTGCTCTTTGTCGACAAGAGATTCCTGAGGATTTTCTTGACAAGCCAACCTTGTTGTCACCAGAAGAACTTAAGGCTGCAAGCAGAGGAAATGGTGAATATGCGTGGTATTATGAAGGAAGGAATGGGTGGTGGCAGTATGATGAGCGCACTAGTCGGGAGCTAGAAGATGCTTTTTCCAAAGGTAAAAAGAACACGGAAATGTTAATTGCTGGATTTCTGTATGTTGCTGATCTTGAAAACATGGTTCAATATAGGAGAAATGAACATGGACGTCGCAGGAAGATTAAGCGAGATATAATAGATATACCAAAGAAGGGAGTAGCTGGACTTAGGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSAPSLTVPECAICLQTCVHPVS
LPCKHVFCYLCVKGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASR
GNGEYAWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEMLIAGFLYVADL
ENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGLR*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX-TEV-(mu)RNF146 Ala30-Arg183724.01 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65351
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 20757; For more information please look into the biochemical assay in the following paper:; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289021/
• Low Temp: No