Full Name
TRAF6
Long Name Textual
TNF receptor-associated factor 6
Synonyms
TRAF6
Immuno Sequence
GST-TRAF6 [DU 1758]
Mono/Poly clonal
Polyclonal
Gel Image
The indicated amounts of anti-TRAF6 antibodies or pre-immune control IgG were incubated for 16 h at 40C on a rotating wheel with 0.025 ml of cell extract (0.5 mg protein) from unstimulated IL-1R HEK293 cells and 7.5 μl of packed Protein G- Sepharose beads. After centrifugation to pellet the antibody-Sepharose conjugate, the supernatants were removed and the immunoprecipitates washed three times with 50 mM Tris-HCl pH 7.5, 1 % (v/v) Triton-X100, 0.05 % (v/v) 2-mercaptoethanol and 0.2 M NaCl. The supernatants and pellets were denatured in SDS and aliquots subjected to SDS-PAGE and immunoblotting using a commercial TRAF6 antibody from Santa Cruz (H-274).
Sheep No
S529C
Unit Source
Aliquot
0.1mg
Price
£150.00