Full Name
DAZAP
Long Name Textual
DAZ associated protein
Immuno Sequence
DAZAP (GST cleaved) [DU 3123]
Mono/Poly clonal
Polyclonal
Gel Image
EK293 cells were pre-incubated for 1 hour with or without 2 μM PD 184352 or 10 μM U0126, then stimulated with 100 g/ml PMA [panel A] or for 10 mins with 100 ng/ml EGF [panel B] and then lysed. Cell lysates were denatured in SDS, subjected to SDS/PAGE, transferred to nitrocellulose membranes and immunoblotted with an antibody that recognises DAZAP1 phosphorylated at Threonine 315 (anti-DAZAP phospho Thr 315 S498B) [upper panels] and with an antibody that recognises the phosphorylated and unphosphorylated forms of DAZAP1 equally well (anti-DAZAP S470B) [lower panels]. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
Sheep No
S470B
Unit Source
Aliquot
0.1mg
Price
£150.00