Full Name
Filamin gamma phospho Ser 2213
Long Name Textual
Filamin
Immuno Sequence
GRERLGS*FGSITR [residues 2207 - 2219 of human]
Mono/Poly clonal
Polyclonal
Gel Image
Murine C2C12 myoblasts were deprived of serum for 12 hours, then stimulated with 250 ng/ml insulin or 20 ng/ml EGF for the times indicated. The cells were lysed and the lysates subjected to SDS/Page followed by transfer to an immobilonP membrane. The membranes were then probed with anti-Filamin phospho Ser 2213 or anti-Filamin (S661B) antibodies. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
Sheep No
S950A
Unit Source
Aliquot
0.1mg
Price
£150.00