cDNA Clone TSC22D2

• Synonyms: TILZ4a; TILZ4b; TILZ4c
• Plasmid: pCMV5 FLAG TSC22D2 V161A
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG TSC22D2 V161A
• Genbank: NM_014779.4
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGCCGGCCAAGAAGAAGAGCTGCTTCCAGATCACCAGTGTCACCACGGCCCAGGTGGCCACTAGCATCACCGAGGACACCGAGAGCTTGGACGACCCGGACGAGTCACGCACAGAGGACGTCTCTTCCGAGATTTTCGACGTCTCTCGGGCCACGGATTATGGCCCTGAGGAGGTCTGCGAGCGCAGCTCTTCCGAAGAGACGCTTAACAATGTTGGGGATGCGGAAACTCCCGGGACCGTCTCCCCAAACCTCCTCCTAGATGGGCAGCTGGCAGCGGCGGCTGCTGCTCCCGCCAACGGAGGAGGAGTCGTTTCGGCCCGGAGCGTGTCTGGGGCGCTCGCCAGTACCCTGGCGGCGGCTGCCACTTCGGCCCCCGCCCCCGGAGCACCCGGCGGCCCCCAGCTCGCGGGCTCATCCGCCGGGCCAGTGACTGCAGCCCCATCTCAGCCTCCCACCACATGTAGTTCCCGTTTTCGCGCGATCAAGCTGGACCACGGGAGCGGAGAGCCCTATAGACGCGGCCGATGGACGTGTATGGAATACTATGAGAGGGATTCAGACAGCAGCGTCCTGACTAGATCCGGGGATTGCATTAGACACAGCAGTACTTTTGACCAGACTGCGGAGCGGGACAGCGGCCTGGGCGCCACCGGAGGGTCGGTGGTGGTAGTAGTGGCCTCCATGCAGGGGGCGCACGGGCCCGAGTCGGGAACTGACAGCTCCTTGACTGCTGTGTCACAGCTACCCCCGTCGGAGAAAATGAGCCAGCCCACTCCGGCCCAGCCGCAGAGTTTTAGCGTTGGGCAGCCACAGCCGCCGCCGCCACCCGTAGGTGGGGCTGTGGCTCAAAGCTCGGCTCCGCTGCCGCCGTTCCCGGGAGCCGCGACCGGGCCGCAGCCAATGATGGCAGCCGCGCAGCCCAGCCAGCCCCAGGGAGCGGGGCCCGGGGGACAGACTCTGCCGCCGACGAATGTAACCCTGGCGCAGCCGGCTATGTCCCTGCCTCCGCAGCCGGGCCCTGCAGTGGGCGCCCCCGCGGCGCAGCAGCCCCAGCAGTTCGCGTATCCTCAGCCTCAGATACCGCCCGGACATTTGCTGCCCGTCCAGCCCTCCGGCCAGAGTGAGTACCTGCAGCAGCACGTGGCCGGCCTGCAGCCGCCAAGCCCCGCGCAGCCCTCGTCCACCGGCGCCGCAGCGAGCCCCGCCACGGCGGCCACCCTTCCCGTGGGCACCGGCCAGAATGCTTCCTCGGTGGGCGCGCAGCTCATGGGCGCGTCTTCCCAGCCCAGCGAAGCCATGGCCCCCCGGACGGGACCAGCGCAAGGCGGGCAGGTCGCGCCTTGTCAGCCGACTGGAGTGCCCCCGGCTACTGTGGGAGGCGTGGTGCAGCCGTGCCTCGGTCCTGCCGGGGCTGGGCAGCCCCAGTCCGTGCCTCCGCCGCAGATGGGTGGCAGTGGTCCGCTGTCAGCCGTACCTGGTGGCCCTCACGCCGTGGTGCCCGGAGTTCCAAACGTGCCTGCAGCCGTGCCCGCTCCAAGCGTGCCTAGTGTGTCTACCACTTCTGTTACTATGCCAAATGTACCCGCGCCTCTGGCCCAGTCGCAACAGCTGAGCAGCCATACGCCAGTCAGCAGGAGCAGCAGCATAATCCAGCATGTTGGGCTGCCCTTAGCGCCAGGCACACACAGCGCACCAACAAGTCTACCACAGTCTGACCTAAGCCAGTTTCAAACTCAGACCCAGCCTTTAGTCGGGCAAGTCGACGATACTAGAAGAAAATCAGAACCCCTACCTCAACCACCACTTTCTCTCATTGCTGAAAATAAGCCTGTTGTGAAGCCGCCTGTTGCAGATTCCCTGGCAAACCCCCTTCAGTTAACACCTATGAACAGTCTGGCCACCTCTGTATTCAGCATAGCTATTCCTGTTGATGGTGATGAAGACAGGAATCCTTCAACTGCTTTCTACCAAGCGTTCCATTTGAACACGTTAAAGGAATCAAAGAGCCTCTGGGATAGTGCATCTGGGGGAGGTGTTGTAGCCATTGACAACAAAATAGAACAAGCAATGGATCTGGTGAAAAGCCATTTGATGTATGCAGTAAGAGAAGAAGTGGAAGTTTTAAAGGAACAAATAAAAGAATTAGTTGAAAGAAACTCTTTACTTGAACGAGAAAATGCACTGTTAAAATCTCTTTCAAGCAATGATCAATTATCCCAACTCCCAACCCAACAGGCCAATCCTGGTAGCACTTCTCAACAGCAAGCAGTGATAGCACAGCCTCCGCAGCCAACGCAACCTCCACAGCAGCCGAATGTCTCCTCAGCATAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMPAKKKSCFQITSVTTAQVATSITEDTESLDDPDESRTEDVSSEIFDVSRATDYGPEEVCERSSSEETLNNVGDAETPGTVSPNLLLDG
QLAAAAAAPANGGGVVSARSVSGALASTLAAAATSAPAPGAPGGPQLAGSSAGPVTAAPSQPPTTCSSRFRAIKLDHGSGEPYRRGRWTCMEYYERDSDS
SVLTRSGDCIRHSSTFDQTAERDSGLGATGGSVVVVVASMQGAHGPESGTDSSLTAVSQLPPSEKMSQPTPAQPQSFSVGQPQPPPPPVGGAVAQSSAPL
PPFPGAATGPQPMMAAAQPSQPQGAGPGGQTLPPTNVTLAQPAMSLPPQPGPAVGAPAAQQPQQFAYPQPQIPPGHLLPVQPSGQSEYLQQHVAGLQPPS
PAQPSSTGAAASPATAATLPVGTGQNASSVGAQLMGASSQPSEAMAPRTGPAQGGQVAPCQPTGVPPATVGGVVQPCLGPAGAGQPQSVPPPQMGGSGPL
SAVPGGPHAVVPGVPNVPAAVPAPSVPSVSTTSVTMPNVPAPLAQSQQLSSHTPVSRSSSIIQHVGLPLAPGTHSAPTSLPQSDLSQFQTQTQPLVGQVD
DTRRKSEPLPQPPLSLIAENKPVVKPPVADSLANPLQLTPMNSLATSVFSIAIPVDGDEDRNPSTAFYQAFHLNTLKESKSLWDSASGGGVVAIDNKIEQ
AMDLVKSHLMYAVREEVEVLKEQIKELVERNSLLERENALLKSLSSNDQLSQLPTQQANPGSTSQQQAVIAQPPQPTQPPQQPNVSSA*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5 FLAG TSC22D2 V161A62.28 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61226
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Compared to reference sequence this clone is missing the first 3 amino acids MSK (ATGTCCAAG) before MPAKKK....SSA*. There are 2 silent changes at S42 and E76. Remake of DU10086.
• Low Temp: No