cDNA Clone Ce_MCM-10

• Synonyms: MCM10
• Plasmid: pMEX 3C C. elegans MCM-10(G470-S691 end)
• Parent Plasmid: pMEX 3C
• Expressed: MBP C. elegans MCM-10(G470-S691 end)
• Genbank: NP_499456.1, NM_067055.4
• Tag: MBP
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGGGTTCAAAGCTATGGACAATGTCACTGGCGAATAAGAAAGCTGCTCCGTTAGGTCATAAAGATGCTCAGGAGCATGCCGCTAGGCTCCGCGCAATCGCAATTCTCAAGGCAAAACGAAAAGAAGCCGAAATGGAGAAGCTCACCGGAGGCGGCGGCGGAGTCAAGCGAAAAGCTTCCGGAACACCGACGGCTCCGGAGCCCGAAGCAAAGAAGCCGAGAAGCCAGAAAATGGATGAGATTCGTGCGATGCTCGCCAGAAAGAGCACACATCACAAGGAAGCGGAGAAGGCGGAGCACGACATGCTCCAGCGTCATTTGACAGGAATGGAAGAACGCGAAAAAGTGGAAACATTTACGACGACTTGTGTTGAAGTTAAAAATGTTAAAGTTGTCACGTGTAATCAGTGCAAATACACATCACAATTCGCATCATCAGAATGTATTAAAAAAGAGCATAAACTCATTAAACACACCGCTGACAAGCGATTCTTCAAATGTACTGGATGCAAAAAACGGACTGTTTGTTTTGAAATGATGCCTGTCAAGCATTGTGCGCACTGCAACGAGAACAAATGGGAGCGCGTAGCGATGAAAGACGAACGAAAAGTGGTTCTCGACACGGAAAATCTGCTGCTCCGCGGTGAAGAACGTCCGTTTGTTGGCTCCTGAGCGGCCGC

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPLGSMGSKLWTMSLANKKAAPLGHKDAQEHAARLRAIAILKAKRKEAEMEKLTGGGGGVKRKASGTPTAPEPEAKKPRSQKMDEIRAMLARKSTHHKEAEKAEHDMLQRHLTGMEEREKVETFTTTCVEVKNVKVVTCNQCKYTSQFASSECIKKEHKLIKHTADKRFFKCTGCKKRTVCFEMMPVKHCAHCNENKWERVAMKDERKVVLDTENLLLRGEERPFVGS*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pMEX 3C C. elegans MCM-10(G470-S691 end)61.59 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66343
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: This was a direct digest of the PCR product generated by amplifying the fragment from a template plasmid given to us by Karim Labib's lab.
• Low Temp: No