cDNA Clone VECTOR

• Plasmid: pET24a-C-(TEV)-DAC VECTOR
• Parent Plasmid: pET24a
• Expressed: C-(TEV)-DAC
• Genbank: xxxxx
• Tag: DAC

Sequence of Insert

GGATCCGAATTCGAGCTCGTCGACAAGCTTGCGGCCGCACTCGAGGAAAA
CCTGTATTTTCAGGGCATGTCTGCAATCCCGGGTGTACCGCAGATCGATG
CGGAGTCCTACATCCTGATTGACTATAACTCCGGCAAAGTGCTCGCCGAA
CAGAACGCAGATGTCCGCCGCGATCCTGCCAGCCTGACCAAAATGATGAC
CAGTTACGTTATCGGCCAGGCAATGAAAGCCGGTAAATTTAAAGAAACTG
ATTTAGTCACTATCGGCAACGACGCATGGGCCACCGGTAACCCGGTGTTT
AAAGGTTCTTCGCTGATGTTCCTCAAACCGGGCATGCAGGTTCCGGTTTC
TCAGCTGATCCGCGGTATTAACCTGCAATCGGGTAACGATGCTTGTGTCG
CCATGGCCGATTTTGCCGCTGGTAGCCAGGACGCTTTTGTTGGCTTGATG
AACAGCTACGTTAACGCACTGGGCCTGAAAAATACCCACTTCCAGACGGT
ACATGGTCTGGATGCTGATGGTCAGTACAGCTCCGCGCGAGATATGGCGC
TGATCGGCCAGGCATTGATCCGTGACGTACCGAATGAATACTCGATCTAT
AAAGAAAAAGAATTTACGTTTAACGGTATTCGCCAGCTGAACCGTAACGG
CCTGTTATGGGATAACAGCCTGAATGTCGACGGCATCAAAACCGGACACA
CTGACAAAGCAGGTTACAACCTTGTTGCTTCTGCGACTGAAGGCCAGATG
CGCTTGATTTCTGCGGTAATGGGCGGACGTACTTTTAAAGGCCGTGAAGC
CGAAAGTAAAAAACTGCTAACCTGGGGCTTCCGTTTCTTTGAAACCGTTA
ACCCAtgactcgag

Amino Acid Sequence

GSEFELVDKLAAALEENLYFQGMSAIPGVPQIDAESYILIDYNSGKVLAE
QNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVF
KGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLM
NSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIY
KEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQM
RLISAVMGGRTFKGREAESKKLLTWGFRFFETVNP*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET24a-C-(TEV)-DAC VECTOR101.61 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65377
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C-ter DAC tagging vector with TEV cleavage. polylinker sites upstream of tag are Bam-Eco-Hind-Not (AAA frame). This plasmid has had the ATG encoded in the vector Nde1 site just upstream of polylinker removed to avoid extraneous N-terminal residues being added to gene of interest. Therefore empty vector does not express.; Parental pET24a; Full vector in sequence of cassette field.; Negative control plasmid for fusions expressed from this vector is 65346 (where expression the vector Nde1 site encoded ATG has been retained).
• Low Temp: No