cDNA Clone WNK1 (kidney Specific)

• Plasmid: pBabe puro FLAG-kidney specific WNK1
• Parent Plasmid: pBabe puro
• Expressed: FLAG-WNK1 (kidney specific)
• Genbank: Q9H4A3-4
• Tag: FLAG
• Species: Human

Sequence of Insert

gaattcATGGATATCAAGAAAAAAGATTTCTGCAGCGTGTTCGTGATCAT
TAACAGCCATTGTTGCTGCTGTCCACAGAAGGATTGCATTAACGAAGGGG
TGAAGCCAGCCAGTTTTGACAAAGTAGCAATTCCTGAAGTGAAGGAAATT
ATTGAAGGATGCATACGACAAAACAAAGATGAAAGATATTCCATCAAAGA
CCTTTTGAACCATGCCTTCTTCCAAGAGGAAACAGGAGTACGGGTAGAAT
TAGCAGAAGAAGATGATGGAGAAAAAATAGCCATAAAATTATGGCTACGT
ATTGAAGATATTAAGAAATTAAAGGGAAAATACAAAGATAATGAAGCTAT
TGAGTTTTCTTTTGATTTAGAGAGAGATGTCCCAGAAGATGTTGCACAAG
AAATGGTAGAGTCTGGGTATGTCTGTGAAGGTGATCACAAGACCATGGCT
AAAGCTATCAAAGACAGAGTATCATTAATTAAGAGGAAACGAGAGCAGCG
GCAGTTGGTACGGGAGGAGCAAGAAAAAAAAAAGCAGGAAGAGAGCAGTC
TCAAACAGCAGGTAGAACAATCCAGTGCTTCCCAGACAGGAATCAAGCAG
CTCCCTTCTGCTAGCACCGGCATACCTACTGCTTCTACCACTTCAGCTTC
AGTTTCTACACAAGTAGAACCTGAAGAACCTGAGGCAGATCAACATCAGC
AACTACAGTACCAGCAACCCAGTATATCTGTGTTATCTGATGGGACGGTT
GACAGTGGTCAGGGATCTTCTGTCTTCACAGAATCTCGAGTGAGCAGCCA
ACAGACAGTTTCATATGGTTCCCAACATGAACAGGCACATTCTACAGGCA
CAGTCCCAGGGCATATACCTTCTACTGTCCAAGCACAGTCTCAGCCCCAT
GGGGTATATCCACCCTCAAGTGTGGCACAGGGGCAGAGCCAGGGTCAGCC
ATCCTCAAGTAGCTTAACAGGGGTTTCATCTTCCCAACCCATACAACATC
CTCAGCAGCAGCAGGGAATACAGCAGACAGCCCCTCCTCAACAGACAGTG
CAGTATTCACTTTCACAGACATCAACCTCCAGTGAGGCCACTACTGCACA
GCCAGTGAGTCAACCTCAAGCTCCACAAGTCTTGCCTCAAGTATCCGCTG
GAAAACAGCTTCCAGTTTCCCAGCCAGTACCAACTATCCAAGGCGAACCT
CAGATCCCAGTTGCGACACAACCCTCGGTTGTTCCAGTCCACTCTGGTGC
TCATTTCCTTCCAGTGGGACAGCCGCTCCCTACTCCCTTGCTCCCTCAGT
ACCCTGTCTCTCAGATTCCCATATCAACTCCTCATGTGTCTACGGCTCAG
ACAGGTTTCTCATCCCTTCCCATCACAATGGCAGCTGGCATTACTCAGCC
TCTGCTCACGTTGGCTTCATCTGCTACAACAGCTGCGATCCCGGGGGTAT
CAACTGTGGTTCCTAGTCAGCTTCCAACCCTTCTGCAGCCTGTGACTCAG
CTGCCAAGTCAGGTTCACCCACAGCTCCTACAACCAGCAGTTCAGTCCAT
GGGAATACCAGCTAACCTTGGACAAGCTGCTGAGGTTCCACTTTCCTCTG
GAGATGTTCTGTACCAGGGCTTCCCACCTCGACTGCCACCACAGTACCCA
GGAGATTCAAATATTGCTCCCTCTTCCAACGTGGCTTCTGTTTGCATCCA
TTCTACAGTCCTATCCCCTCCCATGCCGACAGAAGTACTGGCTACACCTG
GGTACTTTCCCACAGTGGTGCAGCCTTATGTGGAATCAAATCTTTTAGTT
CCTATGGGTGGTGTAGGAGGACAGGTTCAAGTGTCCCAGCCAGGAGGGAG
TTTAGCACAAGCCCCCACTACATCCTCCCAGCAAGCAGTTTTGGAGAGTA
CTCAGGGAGTCTCTCAGGTTGCTCCTGCAGAGCCAGTTGCAGTAGCACAG
CCCCAAGCTACCCAGCCGACCACTTTGGCTTCCTCTGTAGACAGTGCACA
TTCAGATGTTGCTTCAGGTATGAGTGATGGCAATGAGAACGTCCCATCTT
CCAGTGGAAGGCATGAAGGAAGAACTACAAAACGGCATTACCGAAAATCT
GTAAGGAGTCGCTCTCGACATGAAAAAACTTCACGCCCAAAATTAAGAAT
TTTGAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTAGAGA
CTCATAATAGGAAAATGGTTACATTCAAATTTGACCTAGATGGTGACAAC
CCCGAGGAGATAGCAACAATTATGGTGAACAATGACTTTATTCTAGCAAT
AGAGAGAGAGTCGTTTGTGGATCAAGTGCGAGAAATTATTGAAAAAGCTG
ATGAAATGCTCAGTGAGGATGTCAGTGTGGAACCAGAGGGTGATCAGGGA
TTGGAGAGTCTACAAGGAAAGGATGACTATGGCTTTTCAGGTTCTCAGAA
GCTTGAAGGAGAGTTCAAACAACCAATTCCTGCGTCTTCCATGCCACAGC
AAATAGGCATTCCTACCAGTTCTTTAACTCAAGTTGTTCATTCTGCGGGA
AGGCGGTTTATAGTGAGTCCTGTGCCAGAAAGCCGATTACGAGAATCAAA
AGTTTTCCCCAGTGAAATAACAGATACAGTTGCTGCCTCTACAGCTCAGA
GCCCTGGAATGAACTTGTCTCACTCTGCATCATCCCTTAGTCTACAACAG
GCCTTTTCTGAACTTAGACGTGCCCAAATGACAGAAGGACCCAACACAGC
ACCTCCAAACTTTAGTCATACAGGACCAACATTTCCAGTAGTACCTCCTT
TCTTAAGTAGCATTGCTGGAGTCCCAACCACAGCAGCAGCCACAGCACCA
GTCCCTGCAACAAGCAGCCCTCCTAATGACATTTCCACATCAGTAATTCA
GTCTGAGGTTACAGTGCCCACTGAAGAGGGGATTGCTGGAGTTGCCACCA
GCACAGGTGTGGTAACTTCGGGTGGTCTCCCCATACCACCTGTGTCTGAA
TCACCAGTACTTTCCAGCGTAGTTTCAAGTATCACAATACCTGCAGTTGT
CTCAATATCTACTACATCCCCGTCACTTCAAGTCCCCACATCCACATCTG
AGATCGTTGTTTCTAGTACAGCACTGTATCCTTCAGTAACAGTTTCAGCA
ACTTCAGCCTCTGCAGGGGGCAGTACTGCTACCCCAGGTCCTAAGCCTCC
AGCTGTAGTATCTCAGCAGGCAGCAGGCAGCACTACTGTGGGAGCCACAT
TAACATCAGTTTCTACCACCACTTCATTCCCAAGCACAGCTTCACAGCTG
TCCATTCAGCTTAGCAGCAGTACTTCTACTCCTACTTTAGCTGAAACCGT
GGTAGTTAGCGCACACTCACTAGATAAGACATCTCATAGCAGTACAACTG
GATTGGCTTTCTCCCTCTCTGCACCATCTTCCTCTTCCTCTCCTGGAGCA
GGAGTGTCTAGTTATATTTCTCAGCCTGGTGGGCTGCATCCTTTGGTCAT
TCCATCAGTGATAGCTTCTACTCCTATTCTTCCCCAAGCAGCAGGACCTA
CTTCTACACCTTTATTACCCCAAGTACCTAGTATCCCACCCTTGGTACAG
CCTGTTGCCAATGTGCCTGCTGTACAGCAGACACTAATTCATAGTCAGCC
TCAACCAGCTTTGCTTCCCAACCAGCCCCATACTCATTGTCCTGAAGTAG
ATTCTGATACACAACCCAAAGCTCCTGGAATTGATGACATAAAGACTCTA
GAAGAAAAGCTGCGGTCTCTGTTCAGTGAACACAGCTCATCTGGAGCTCA
GCATGCCTCTGTCTCACTGGAGACCTCACTAGTCATAGAGAGCACTGTCA
CACCAGGCATCCCAACTACTGCTGTTGCACCAAGCAAACTCCTGACTTCT
ACCACAAGTACTTGCTTACCACCAACCAATTTACCACTAGGAACAGTTGC
TTTGCCAGTTACACCAGTGGTCACACCTGGGCAAGTTTCTACCCCAGTCA
GCACTACTACATCAGGAGTGAAACCTGGAACTGCTCCCTCCAAGCCACCT
CTAACTAAGGCTCCGGTGCTGCCAGTGGGTACTGAACTTCCAGCAGGTAC
TCTACCCAGCGAGCAGCTGCCACCTTTTCCAGGACCTTCTCTAACCCAGT
CCCAGCAACCTCTAGAGGATCTTGATGCTCAATTGAGAAGAACACTTAGT
CCAGAGATTATCACAGTGACTTCTGCGGTTGGTCCTGTGTCCATGGCGGC
TCCAACAGCAATCACAGAAGCAGGAACACAGCCTCAGAAGGGTGTTTCTC
AAGTCAAAGAAGGCCCTGTCCTAGCGACTAGTTCAGGAGCTGGTGTTTTT
AAGATGGGACGATTTCAGGTTTCTGTTGCAGCAGACGGTGCCCAGAAAGA
GGGTAAAAATAAGTCAGAAGATGCAAAGTCTGTTCATTTTGAATCCAGCA
CCTCAGAGTCCTCAGTGCTATCAAGTAGTAGTCCAGAGAGTACCTTGGTG
AAACCAGAGCCGAATGGCATAACCATCCCTGGTATCTCTTCAGATGTGCC
AGAGAGTGCCCACAAAACTACTGCCTCAGAGGCAAAGTCAGACACTGGGC
AGCCTACCAAGGTTGGACGTTTTCAGGTGACAACTACAGCAAACAAAGTG
GGTCGTTTCTCTGTATCAAAAACTGAGGACAAGATCACTGACACAAAGAA
AGAAGGACCAGTGGCATCTCCTCCTTTTATGGATTTGGAACAAGCTGTTC
TTCCTGCTGTGATACCAAAGAAAGAGAAGCCTGAACTGTCAGAGCCTTCA
CATCTAAATGGGCCGTCTTCTGACCCGGAGGCCGCTTTTTTAAGTAGGGA
TGTGGATGATGGTTCCGGTAGTCCACACTCGCCCCATCAGCTGAGCTCAA
AGAGCCTTCCTAGCCAGAATCTAAGTCAAAGCCTTAGTAATTCATTTAAC
TCCTCTTACATGAGTAGCGACAATGAGTCAGATATCGAAGATGAAGACTT
AAAGTTAGAGCTGCGACGACTACGAGATAAACATCTCAAAGAGATTCAGG
ACCTGCAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACCAAACTG
GGCAAGGTGCCCCCTGCTGTTATTATTCCCCCAGCTGCTCCCCTTTCAGG
GAGAAGACGACGACCCACTAAAAGCAAAGGCAGCAAATCTAGTCGAAGCA
GTTCCTTGGGGAATAAAAGCCCCCAGCTTTCAGGTAACCTGTCTGGTCAG
AGTGCAGCTTCAGTCTTGCACCCCCAGCAGACCCTCCACCCTCCTGGCAA
CATCCCAGAGTCCGGGCAGAATCAGCTGTTACAGCCCCTTAAGCCATCTC
CCTCCAGTGACAACCTCTATTCAGCCTTCACCAGTGATGGTGCCATTTCA
GTACCAAGCCTTTCTGCTCCAGGTCAAGGAACCAGCAGCACAAACACTGT
TGGGGCAACAGTGAACAGCCAAGCCGCCCAAGCTCAGCCTCCTGCCATGA
CGTCCAGCAGGAAGGGCACATTCACAGATGACTTGCACAAGTTGGTAGAC
AATTGGGCCCGAGATGCCATGAATCTCTCAGGCAGGAGAGGAAGCAAAGG
GCACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCACCTG
GGCAACTGTGCATCTCCATGACCTCGAACCTGGGTGGCTCTGCCCCCATC
TCTGCAGCATCAGCTACCTCTCTAGGTCACTTCACCAAGTCTATGTGCCC
CCCACAGCAGTATGGCTTTCCAGCTACCCCATTTGGCGCTCAATGGAGTG
GGACGGGTGGCCCAGCACCACAGCCACTTGGCCAGTTCCAACCTGTGGGA
ACTGCCTCCTTGCAGAATTTCAACATCAGCAATTTGCAGAAATCCATCAG
CAACCCCCCAGGCTCCAACCTGCGGACCACTTAGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSSEFMDIKKKDFCSVFVIINSHCCCCPQKDCINEGVKPAS
FDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEED
DGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVES
GYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQV
EQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQ
QPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGH
IPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQ
GIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLP
VSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQ
IPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVP
SQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLY
QGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPT
VVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVS
QVAPAEPVAVAQPQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRH
EGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRK
MVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLS
EDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIP
TSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMN
LSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSI
AGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVV
TSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVS
STALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVS
TTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFS
LSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPL
LPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQ
PKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIP
TTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTS
GVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPL
EDLDAQLRRTLSPEIITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEG
PVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESS
VLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKV
GRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVI
PKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPS
QNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSR
QKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGN
KSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDN
LYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRK
GTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCI
SMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGP
APQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65274
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: This is a kidney specific isoform of WNK1. It is a kinase-defective isoform produced by alternative promoter usage and alternative splicing. It lacks residues 1-407, and residues 408-437 are different to WT. Sequence is available on uniprot as isoform 3 of WNK1 (identifier: Q9H4A3-5); Parental 37983; Grow at 30 degrees
• Low Temp: Yes