cDNA Clone ULK1

• Plasmid: pcDNA5D FRT/TO-2x(6His)-ULK1 kinase domain
• Parent Plasmid: pcDNA5D FRT/TO
• Expressed: 2x(6His)-ULK1 kinase domain
• Genbank: NM_003565.3
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccATGcatcatcatcatcatcatagcagcggcctggtgccgcgcgg
cagccatatggcgagccatcatcatcatcatcatcatcatcatcatggcg
aaaacctgtattttcagggcGAGTTCTCCCGCAAGGACCTGATCGGCCAC
GGCGCCTTCGCGGTGGTCTTCAAGGGCCGCCACCGCGAGAAGCACGATTT
GGAGGTCGCCGTCAAGTGCATTAACAAGAAGAACCTCGCCAAGTCTCAGA
CGCTGCTGGGGAAGGAAATCAAAATCCTGAAGGAACTGAAACATGAAAAC
ATCGTGGCCCTGTACGACTTCCAGGAAATGGCTAATTCTGTCTACCTGGT
TATGGAGTACTGCAACGGTGGGGACCTGGCCGACTACCTGCACGCCATGC
GCACGCTGAGCGAGGACACCATCAGGCTCTTCCTGCAGCAGATCGCGGGC
GCCATGCGGCTTCTGCACAGCAAAGGCATCATCCACCGCGACCTGAAACC
GCAGAACATCCTGCTGTCCAACCCCGCCGGCCGCCGCGCCAACCCCAACA
GCATCCGCGTCAAGATCGCTGACTTCGGCTTCGCGCGGTACCTCCAGAGC
AACATGATGGCGGCCACACTCTGCGGCTCCCCCATGTACATGGCCCCCGA
GGTCATCATGTCCCAGCACTACGACGGGAAGGCGGACCTGTGGAGCATCG
GCACCATCGTCTACCAGTGCCTGACGGGGAAGGCGCCCTTCCAGGCCAGC
AGCCCCCAGGACCTGCGCCTGTTCTACGAGAAGAACAAGACGTTGGTCCC
CACCATCCCCCGGGAGACCTCGGCCCCGCTGCGGCAGCTGCTCCTGGCCC
TACTGCAACGCAACCACAAGGACCGCATGGACTTCGATGAGTTTTTTCAT
CACCCTTTCtgaGCGGCCGC

Amino Acid Sequence

MHHHHHHSSGLVPRGSHMASHHHHHHHHHHGENLYFQGEFSRKDLIGHGA
FAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIV
ALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAM
RLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNM
MAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSP
QDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHP
F*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65169
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 6HIS-Thrombin-6His-TEV-ULK1 kinase domain (comprising Glu15-Phe277); Parental 41459
• Low Temp: No