cDNA Clone ULK1

• Synonyms: ATG1; ATG1A; hATG1; UNC51; Unc51.1
• Plasmid: pBabe.puro.3xHA-TURBO-ID-ULK1
• Parent Plasmid: pBabeD puro 3xHA
• Expressed: 3xHA TURBO-ID-ULK1
• Genbank: NM_003565.2 GI:225637564 with silent change c1065t and coding change c2141t (P714L) (exisiting SNP)
• Tag: HA
• Species: Human

Sequence of Insert

AGATCTATGAAAGACAATACTGTGCCTCTGAAGCTGATCGCTCTCCTGGC
TAATGGCGAGTTCCATAGTGGCGAACAGCTGGGAGAAACCCTGGGCATGT
CCAGGGCCGCTATCAACAAGCACATTCAGACTCTGCGCGACTGGGGCGTG
GACGTGTTCACCGTGCCCGGAAAGGGCTACTCTCTGCCCGAGCCTATCCC
GCTGCTGAACGCTAAACAGATTCTGGGACAGCTGGACGGCGGGAGCGTGG
CAGTCCTGCCTGTGGTCGACTCCACCAATCAGTACCTGCTGGATCGAATC
GGCGAGCTGAAGAGTGGGGATGCTTGCATTGCAGAATATCAGCAGGCAGG
GAGAGGAAGCAGAGGGAGGAAATGGTTCTCTCCTTTTGGAGCTAACCTGT
ACCTGAGTATGTTTTGGCGCCTGAAGCGGGGACCAGCAGCAATCGGCCTG
GGCCCGGTCATCGGAATTGTCATGGCAGAAGCGCTGCGAAAGCTGGGAGC
AGACAAGGTGCGAGTCAAATGGCCCAATGACCTGTATCTGCAGGATAGAA
AGCTGGCAGGCATCCTGGTGGAGCTGGCCGGAATAACAGGCGATGCTGCA
CAGATCGTCATTGGCGCCGGGATTAACGTGGCTATGAGGCGCGTGGAGGA
AAGCGTGGTCAATCAGGGCTGGATCACACTGCAGGAAGCAGGGATTAACC
TGGACAGGAATACTCTGGCCGCTACGCTGATCCGAGAGCTGCGGGCAGCC
CTGGAACTGTTCGAGCAGGAAGGCCTGGCTCCATATCTGCCACGGTGGGA
GAAGCTGGATAACTTCATCAATAGACCCGTGAAGCTGATCATTGGGGACA
AAGAGATTTTCGGGATTAGCCGGGGGATTGATAAACAGGGAGCCCTGCTG
CTGGAACAGGACGGAGTTATCAAACCCTGGATGGGCGGAGAAATCAGTCT
GCGGTCTGCCGAAAAGGGATCC

Amino Acid Sequence

MYPYDVPDYAYPYDVPDYAYPYDVPDYAGSMKDNTVPLKLIALLANGEFH
SGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIPLLNAK
QILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACIAEYQQAGRGSRG
RKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADKVRV
KWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQ
GWITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNF
INRPVKLIIGDKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK
GSLEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCI
NKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGG
DLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSN
PAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHY
DGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETS
APLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSS
GSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSK
DSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESH
GRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNL
QSPTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGG
GRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTS
GLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQS
CRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLAR
QGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRL
TDLLLKAAFGTQAPDLGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSS
PSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAP
APELPAPGHGCSFADPIAANLEGAVTFEAPDLPEETLMEQEHTEILRGLR
FTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAE
QLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVV
SCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQ
HREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGI
CA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65036
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 29961 ; Grow at 30 degrees.; silent change c1065t and coding change c2141t (P714L) (exisiting SNP)
• Low Temp: No