cDNA Clone Zuo1

• Plasmid: pRS413 ADH Pr 400-ZUO1/YGR285C -200 S50A
• Parent Plasmid: pRS413 ADH Pr
• Expressed: ZUO1/YGR285C S50A

Sequence of Insert

GGATCCGCCTTGTACTTTCAACCGTTCATTTCATTAAGAACTTGACTAAATATGAACATTTCTTAAAAAAAAGGTTGACATATAAAAATAATCGAATATAAACGATGGAATTTTTATAAAATTAAACACATATATATATATATATTAACTATAAATATGTCAAAGAAACCATACAATCATAGATTTATAACTATCTTTTGGATGACATTAATGAACATAACGCTCCTAATACAAATGTCCAAAAAATATTACCCGCAAATACGAATCTTTTTTTTTTCTCGATGAAATTTTGCAAAGAGTTCGAAATTTTTATTTCAAGAGCTGGTAGAGAAAATTTCATAAGGTTTTCCTACCGATGCTTTTATAAAATCTTCGTTTTGTCTCACATATACCAACAAGAGTAACGATGTTTTCTTTACCTACCCTAACCTCAGACATCACTGTTGAAGTCAACAGTTCCGCTACCAAAACCCCATTCGTCCGTCGTCCGGTCGAACCGGTTGGTAAGTTCTTTTTGCAACATGCTCAAAGAACTTTGAGAAACCACACCTGGGCTGAATTTGAAAGAATTGAAGCTGAAAAGAACGTCAAAACCGTTGATGAATCCAATGTCGACCCAGATGAGTTGTTATTCGACACTGAATTGGCCGATGAAGATTTACTGACTCATGATGCTAGAGACTGGAAAACTGCCGATTTGTATGCTGCTATGGGTTTGTCTAAGTTGCGTTTCAGAGCTACTGAAAGTCAAATCATCAAGGCTCACAGAAAACAAGTTGTCAAGTACCATCCAGACAAGCAATCTGCTGCTGGTGGTAGTTTGGACCAAGATGGCTTTTTCAAGATTATTCAAAAGGCCTTTGAAACTTTGACTGATTCCAACAAGAGAGCTCAGTACGACTCATGTGATTTTGTTGCCGATGTTCCTCCTCCAAAGAAGGGTACCGATTATGACTTTTATGAAGCTTGGGGCCCCGTTTTCGAAGCTGAAGCTCGTTTTTCTAAGAAGACTCCTATTCCTTCTCTAGGTAACAAAGATTCTTCCAAGAAGGAAGTTGAACAATTCTATGCTTTCTGGCACAGATTTGACTCCTGGAGAACCTTTGAGTTCTTGGACGAAGATGTCCCAGATGACTCTTCTAACAGAGACCACAAGCGTTACATTGAAAGAAAGAACAAGGCCGCAAGAGACAAGAAGAAGACTGCTGATAACGCTAGATTGGTCAAACTTGTTGAAAGAGCTGTCAGTGAAGATCCCCGTATCAAAATGTTCAAAGAAGAAGAGAAGAAGGAAAAGGAAAGAAGAAAATGGGAAAGAGAAGCCGGTGCCAGAGCTGAAGCTGAAGCTAAGGCCAAGGCCGAAGCTGAAGCGAAGGCTAAAGCTGAATCTGAAGCCAAGGCTAACGCCTCCGCAAAAGCTGACAAAAAGAAGGCTAAGGAAGCTGCTAAGGCCGCCAAGAAAAAGAACAAGAGAGCCATCCGTAACTCTGCTAAGGAAGCTGACTACTTTGGTGATGCTGACAAGGCCACCACGATTGACGAACAAGTTGGTTTGATCGTTGACAGTTTGAATGACGAAGAGTTAGTGTCCACCGCCGATAAGATCAAGGCCAATGCTGCTGGTGCCAAGGAAGTTTTGAAGGAATCTGCAAAGACTATTGTCGATTCTGGCAAACTACCATCCAGCTTGTTGTCCTACTTCGTGTGAATACCGTAAGAAATGGAATAGAATATATACGAATGTATACGAATATTATAGAGAACGTTCTCTTTTATTTCTATAATGAATAGGTTCGGGTAACGGTTCCCTTTTTAGGTATTTCTAGAAGATGAGAGAAGAGGGAATAATGAGAAAGGCGAAAAATAAAGGACACCTTTAACGAAAGATCAAAGGTGTCCTTATTTACCTCGAG

Amino Acid Sequence

MFSLPTLTSDITVEVNSSATKTPFVRRPVEPVGKFFLQHAQRTLRNHTWAEFERIEAEKNVKTVDESNVDPDELLFDTELADEDLLTHDARDWKTADLYAAMGLSKLRFRATESQIIKAHRKQVVKYHPDKQSAAGGSLDQDGFFKIIQKAFETLTDSNKRAQYDSCDFVADVPPPKKGTDYDFYEAWGPVFEAEARFSKKTPIPSLGNKDSSKKEVEQFYAFWHRFDSWRTFEFLDEDVPDDSSNRDHKRYIERKNKAARDKKKTADNARLVKLVERAVSEDPRIKMFKEEEKKEKERRKWEREAGARAEAEAKAKAEAEAKAKAESEAKANASAKADKKKAKEAAKAAKKKNKRAIRNSAKEADYFGDADKATTIDEQVGLIVDSLNDEELVSTADKIKANAAGAKEVLKESAKTIVDSGKLPSSLLSYFV*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU66097
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Zuo1 S50A mutation in PRS413 Zuo1 (DU59842)
• Low Temp: No