cDNA Clone UBP5/YER144C

• Plasmid: pRS413 ADH Pr 400-UBP5/YER144C-200
• Parent Plasmid: pRS413 ADH Pr
• Expressed: UBP5/YER144C
• Genbank: (ID:856887)
• Species: Yeast

Sequence of Insert

AGAACTACGAACTTCCCGAGCAAACTACCAGTCATTCCTCGTCTCAAAACGTCGCCACAGACAAGGAAGTCCTCCACGTTAAAGCCGACTCTCTTTGAATGAAGTCTAGCACAGGGTGGGAGTTTTCCACCTATTTTCTGTTGATAAAAATACTACATTTATATACATATATACAATAATTTCTAATATCGTTTTTATAAAGTTGGCCTTGTCTGTACGGCGTCGCAAAACAAGAACCCCTGGTCCAATTGATCTACCGGGCATTGATTTTTTTTCTTTTTTCCTTGCGCCTCACTTCAACACATCAAGTAAACACCAGCTATATTTAGTTGCTAGGTATTCTCCAACGTATGTGGAGTCCTTTCTTTATTATCTCTCTACACAAATAGGCACAAGCGTAATGGGCTCAGAACAAGCCTTAAGTGAGGTTGTCGAATCTGCTAAAGAGCGGTTCGGCAGGCTTAGGCATTTAGTTCAGAAGTTTCTTGATGATGACGACGTGCCACAAGAGTGTCTGCCTTTGCTCCAAGAATGCGCAGAAATATGGTCTAGTTATGTGGACGCATGCCAGGATATAACCATGCAGGCCCCTAAAGAAGATGCAAACCGGTTGTCAAAGGGGTTTCTCCGATTGAATGAAACAGCTTTTTTATATTACATGATCGTATATACGCTCTTGGAGGATACTCTACCAAGACTAAAGGAATTTTCGAGCAACAAGGACCAGAATGTTAGAAATTTGTACGGGGAGAGAATCCAGCTTTTGCATAATGACCCAAATATTGAGCGGATTCGAAATGTCATTGAAAATTACCCTAAATTTATACAGTTGCAAACTATAGAGCCTGGAAAATTAAGTTCAATGTTGCATTTTCATGGTGACGCTCTCTTGCTTATCGATGTACGGCCCAGGTCTGAATTCGTTAGGGCCCATATCAAATGCAAAAACATTATATGCATCGATCCTGCCTCATTTAAGGATTCTTTTACAGACCAACAAATTGAAAGTGTCTCTTTGATCACTTCGCCGCACTCAGATATCACTTTCTTTTCAAATCGAGACAAGTTCAAGTTCATTATTTTATATACTGACACTCAATTGCATAATAATTTCCAGCAAAGGCAAACTCGCATCCTTGCGAAGATTCTTTCCCAAAATTCAGTAATTAAACCGCTGAGTGGGACGAAAATACTCATTTTGGAAAATGGGTTCTCAAACTGGGTTAAATTAGGTGGGGCGTATCAATCATCTGTCAGTGAAACAGCGCATCTTACATCCTCGTCTTCAACGCCAGCTTTTGGTAGTCCGCAAGTCCCAACAGGCTTGTTCAATCAGAAATCTCTATCTCCAAATAAAGATAAGAGTATGCCGATGGTATCCATGAATACGCAGCCTTTGCTAACAACGGTGCAAAGGCCTCAATTACCCTTATACTATTCAGATTTGCCCATTATACCGCAACCAAGCCCCAACAGAAATTCTCCCACCGTTCAAAAATTTAGTCCACATCCACCAACCACTCTCTCAAAACTCAATACACCCTCGACCATTCAAAATAAAGCAAACACTGTGGAAAGAATATCGCCAGATATCCGCGCTGCACAAGCCCATGCCTATTTGCCCCCTGCATCGAATGTATTCTCGCCACGTATTCCGCCTTTACCACAACAGAACTTGTCTTCGTCGAGGCAGACCATCCTAAACAACTCACAAGTGCTTGATCTAGATCTTATAGTAGGATTGGAAAATATAGGTAACTGTTGTTATATGAACTGTATACTTCAATGTTTGGTGGGAACACACGATTTGGTTCGAATGTTTTTGGATAACACATATCTGAATTTTATTAATTTTGATAGTTCAAGAGGTTCTAAGGGGTTGCTAGCTAAAAATTTCGCCATCTTGGTCAATAATATGCACAGACACGGTGCATTCACACCTCCTAACGTAAGAACGATACCAGTTCAGACTATTCAGTTTAAAAAAATTTGCGGCCATATCAACCCCATGTATAGTGATTCAATGCAGCAAGATTGTCAAGAATTTTGTCAATTTTTGCTTGATGGCTTGCACGAGGATTTGAACCAAAACGGCAGCAAAAAGCATCTTAAACAGTTATCAGATGAGGAAGAACGAATGAGAGAAAAAATGTCCATACGCAAAGCTAGTGCTCTCGAGTGGGAAAGATTTTTGCTTACCGACTTCAGTGCGATAATTGACCTTTTCCAAGGACAGTACGCATCTAGGCTACAATGTCAAGTTTGTGAACATACCTCCACAACTTACCAAACATTCTCTGTTCTTTCTGTTCCTGTCCCACGCGTTAAAACTTGTAACATATTAGACTGTTTCCGGGAATTCACCAAATGCGAAAGGTTAGGTGTCGATGAACAATGGTCATGTCCTAAATGCTTAAAAAAGCAGCCTTCCACTAAACAACTGAAGATTACTAGATTGCCTAAGAAACTAATTATTAATTTGAAACGATTTGACAATCAAATGAATAAGAATAATGTGTTTGTCCAATATCCTTACTCCTTAGATCTTACACCATATTGGGCGAGAGATTTTAATCATGAAGCTATTGTTAATGAGGACATTCCTACCAGGGGCCAAGTACCACCATTTAGATACAGACTGTATGGGGTTGCATGTCATTCGGGGAGTTTGTATGGGGGACACTATACTTCATACGTTTATAAGGGACCAAAAAAAGGTTGGTATTTTTTCGATGACTCGCTTTATCGTCCTATAACGTTTAGTACTGAATTTATCACACCCAGTGCATATGTTTTATTTTACGAAAGAATTTTTTGAATCAAATTACCGGTTTGCAACACATGAATGGCGCACATTGAGGCACAAATACAATCTGCATATTATATACTTAACAGAAGTACAATCATATACAATACAAACAACAATTGACGAAACAATGAAAGTATGTACATCCTTATACTTATCTATTTGTGTTATGGGCTACATACGTAGAGGCCGATCACAATATCAGTGGTTGC

Amino Acid Sequence

MGSEQALSEVVESAKERFGRLRHLVQKFLDDDDVPQECLPLLQECAEIWSSYVDACQDITMQAPKEDANRLSKGFLRLNETAFLYYMIVYTLLEDTLPRLKEFSSNKDQNVRNLYGERIQLLHNDPNIERIRNVIENYPKFIQLQTIEPGKLSSMLHFHGDALLLIDVRPRSEFVRAHIKCKNIICIDPASFKDSFTDQQIESVSLITSPHSDITFFSNRDKFKFIILYTDTQLHNNFQQRQTRILAKILSQNSVIKPLSGTKILILENGFSNWVKLGGAYQSSVSETAHLTSSSSTPAFGSPQVPTGLFNQKSLSPNKDKSMPMVSMNTQPLLTTVQRPQLPLYYSDLPIIPQPSPNRNSPTVQKFSPHPPTTLSKLNTPSTIQNKANTVERISPDIRAAQAHAYLPPASNVFSPRIPPLPQQNLSSSRQTILNNSQVLDLDLIVGLENIGNCCYMNCILQCLVGTHDLVRMFLDNTYLNFINFDSSRGSKGLLAKNFAILVNNMHRHGAFTPPNVRTIPVQTIQFKKICGHINPMYSDSMQQDCQEFCQFLLDGLHEDLNQNGSKKHLKQLSDEEERMREKMSIRKASALEWERFLLTDFSAIIDLFQGQYASRLQCQVCEHTSTTYQTFSVLSVPVPRVKTCNILDCFREFTKCERLGVDEQWSCPKCLKKQPSTKQLKITRLPKKLIINLKRFDNQMNKNNVFVQYPYSLDLTPYWARDFNHEAIVNEDIPTRGQVPPFRYRLYGVACHSGSLYGGHYTSYVYKGPKKGWYFFDDSLYRPITFSTEFITPSAYVLFYERIF*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU59809
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of UBP5 including 400bp of the 5'UTR and 200bp of the 3'UTR in the vector pRS413-ADH-Pr
• Low Temp: No