cDNA Clone WDTC1

• Synonyms: ADP; DCAF9
• Plasmid: pcDNA5 FRT/TO GFP NLS WDTC1
• Parent Plasmid: pcDNA5 FRT/TO GFP NLS
• Expressed: GFP NLS WDTC1
• Genbank: NM_001276252.1 with delta E317*
• Tag: NLS
• Species: Human

Sequence of Insert

GGATCCATGGCGAAAGTCAACATAACTAGAGACCTCATCCGTAGGCAGATCAAGGAGCGGGGTGCCCTGAGCTTTGAGCGGCGCTACCATGTCACTGACCCCTTTATCCGGCGGCTGGGCCTGGAAGCAGAGCTGCAGGGTCACTCAGGATGTGTCAACTGTCTGGAGTGGAATGAGAAAGGAGACTTGCTGGCCTCTGGTTCCGATGACCAGCACACGATTGTGTGGGACCCGCTGCACCACAAGAAGCTGCTCTCCATGCACACGGGACACACCGCAAATATCTTCTCTGTCAAGTTCCTGCCTCACGCTGGGGACCGCATCTTGATCACGGGGGCAGCCGACTCTAAGGTGCATGTGCACGACCTGACAGTAAAGGAGACCATCCACATGTTTGGAGACCACACAAACCGGGTGAAGCGCATCGCCACAGCGCCCATGTGGCCCAACACATTCTGGAGTGCTGCTGAGGATGGGCTTATCCGCCAGTATGACCTTCGAGAGAACAGCAAACACTCGGAGGTGCTGATTGACCTGACAGAGTACTGTGGCCAGCTGGTGGAGGCCAAGTGCCTCACTGTCAACCCCCAGGACAACAACTGCCTGGCAGTTGGGGCCAGCGGGCCCTTCGTGAGGCTCTATGACATCCGCATGATCCATAACCACAGAAAGAGCATGAAGCAGAGCCCTTCAGCGGGTGTGCACACCTTCTGTGACCGGCAGAAACCCCTTCCGGACGGTGCAGCCCAGTATTACGTAGCAGGTCACCTGCCAGTGAAGCTTCCTGACTACAACAACCGTTTGAGAGTGCTGGTTGCCACCTATGTGACCTTCAGCCCCAATGGCACAGAGCTACTAGTCAACATGGGGGGGGAACAGGTCTATTTGTTTGACTTGACTTACAAGCAGCGGCCGTACACCTTCCTCTTGCCTAGAAAATGCCACTCCTCGGGGGTCCAGAATGGCAAGATGTCCACCAACGGTGTGTCCAACGGTGTGTCCAATGGCCTGCACCTTCATAGCAATGGCTTCCGGCTGCCGGAGAGTAGGGGACATGTCAGCCCCCAAGTAGAGCTACCACCATACCTGGAGCGTGTGAAACAGCAAGCCAATGAGGCTTTTGCCTGCCAGCAGTGGACCCAAGCCATTCAGCTTTACAGCAAGGCTGTGCAGAGGGCCCCTCACAATGCCATGCTTTATGGAAACCGAGCAGCAGCCTACATGAAGCGCAAGTGGGATGGTGACCACTATGATGCCCTGAGGGACTGCCTCAAGGCCATCTCCCTAAACCCATGCCACCTGAAGGCACACTTTCGCCTGGCCCGCTGCCTCTTTGAGCTCAAGTATGTGGCTGAAGCCCTGGAGTGCCTGGACGACTTCAAAGGGAAATTTCCGGAGCAGGCCCACAGCAGCGCTTGTGATGCATTGGGCCGCGACATCACAGCTGCCCTCTTCTCTAAAAATGATGGTGAGGAGAAGAAGGGACCTGGTGGCGGCGCCCCAGTCCGCCTCCGCAGCACGAGCCGCAAGGACTCCATCTCAGAGGATGAAATGGTGCTGCGGGAGCGAAGCTACGACTATCAGTTCCGCTACTGCGGCCACTGCAACACCACCACGGATATCAAAGAGGCCAATTTCTTTGGCAGCAACGCTCAGTATATCGTCAGTGGCTCTGACGATGGCTCCTTCTTCATCTGGGAAAAGGAGACCACCAACCTGGTCCGTGTGCTCCAAGGGGATGAGTCCATTGTCAACTGCCTGCAGCCACACCCCAGCTACTGCTTCCTGGCCACCAGTGGCATCGATCCTGTTGTGCGGCTCTGGAACCCCCGACCAGAGAGTGAAGACCTCACAGGCCGAGTCGTGGAAGATATGGAGGGTGCTTCACAGGCCAACCAGCGGCGCATGAATGCAGACCCGTTGGAGGTGATGCTGCTCAACATGGGCTACCGGATCACGGGCCTGAGCAGTGGGGGTGCCGGGGCCTCTGATGATGAGGACAGCTCTGAGGGCCAGGTGCAGTGCCGGCCCAGCTAGGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLPPKKKRKVGSMAKVNITRDLIRRQIKERGALSFERRYHVTDPFIRRLGLEAELQGHSG
CVNCLEWNEKGDLLASGSDDQHTIVWDPLHHKKLLSMHTGHTANIFSVKFLPHAGDRILITGAADSKVHVHDLTVKETIHMFGDHTNRVKRIATAPMWPN
TFWSAAEDGLIRQYDLRENSKHSEVLIDLTEYCGQLVEAKCLTVNPQDNNCLAVGASGPFVRLYDIRMIHNHRKSMKQSPSAGVHTFCDRQKPLPDGAAQ
YYVAGHLPVKLPDYNNRLRVLVATYVTFSPNGTELLVNMGGEQVYLFDLTYKQRPYTFLLPRKCHSSGVQNGKMSTNGVSNGVSNGLHLHSNGFRLPESR
GHVSPQVELPPYLERVKQQANEAFACQQWTQAIQLYSKAVQRAPHNAMLYGNRAAAYMKRKWDGDHYDALRDCLKAISLNPCHLKAHFRLARCLFELKYV
AEALECLDDFKGKFPEQAHSSACDALGRDITAALFSKNDGEEKKGPGGGAPVRLRSTSRKDSISEDEMVLRERSYDYQFRYCGHCNTTTDIKEANFFGSN
AQYIVSGSDDGSFFIWEKETTNLVRVLQGDESIVNCLQPHPSYCFLATSGIDPVVRLWNPRPESEDLTGRVVEDMEGASQANQRRMNADPLEVMLLNMGY
RITGLSSGGAGASDDEDSSEGQVQCRPS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5 FRT/TO GFP NLS WDTC173.18 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61059
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Delta E317 *As seen in other published sequences for this protein.
• Low Temp: No