cDNA Clone YMR310C

• Plasmid: pRS413 ADH Pr -600-YMP310C-(+)300
• Parent Plasmid: pRS413 ADH Pr
• Expressed: yeast YMR310C including 600 bp promoter and 300 bp 3'UTR

Sequence of Insert

GGATCCACATAGACGAGCCCAAGACCCCCTACCAAGGTGCTGTGGACCCTCACGGGGAATACTACAGAGTAGATGATGATGAAGATGAGGATAATAGTGATAAAAAACCATGCCAGGTGGCAAACGATGACATCGACGATTTGTCCCTGGGCGAACCGGAATTTGAAATCAAAGAGAACAAACAACCGGATTTTGAGACCAACGACGAGAATGATGAAGACTCACCGGAGGCTAGACATAAGAAATTTGAAGAAATGAGAAAGAAGCATTACGACGTAAGGGCAATTTTCAACAAAAAGTCCCGTGAAGCGCTAAAGGACGAAGACGAAGACGAAGATGATAGTACGACAAAAGAACAATGAAACATGACACCTCCATAACGTTTACCTATACTAAATATATCTTATTTTTTGGATTCTTGGTACATAAATATGCACTGTTTGTTATCACGCGAAACGAACTGATTTTTTAACAAGCTTTATTTTATACATGAAAAATTTCCCATCGATGAGCTGTGATCGGCAAGTGAGAGTACTCTGTTTCACAACTACTACCGGTCCCAGGAATATATACTACTGACCACAAAGCTGTGTATCACTTAGTTTGATGAGCAGCACGAGGAAGTTCAAAAAGGTTGAGAAACCTCTATCACAAACCAGACATTATTCACTCTGTATCCCCACGACATTGGTATCGGACTGTCGCAACCTCTCTCAAATCACGCATAAGGTGTACCAAGTGGCCAAGTTTGCATCGCTGTTTAATGTTTCTGAGGTTGTCATTCTTGAGGACAATTCCCAGGTCGATGCCACCAAAAAAAAAATCTCCACCGCTAAATTAATACTGGCACTTTTACAATACTTCGTCACGCCTCCATATCTTCGCAATACTGTCTTCAACGAAAAATTCAGACCTTATTTGACCGCCGCTTCAAAATTACCAAGATTATCCACACTTCCTTTCACCAGGTACCAAAAGCAAGATCACGGTCGGTACAGAGAAGGCCTCACTATCAAGATGCAGAAACCCACCTTGGCTCGCAAAAAGACTGGTAAGGTATTCAAGCAAACCAAATATATTAATATAGGGAAAAGCAAAGCACTGGCTCTACAAAGTCAGCTGGTGCCGATAAACGCAAGGGTCACCATCGACACAATAACGAGGAAAATTGTATCTCCTCAGGAAGCTTACGGTGATTTCACCGGATTGGAGTCGCAGTATGGCTACTATACTCGTATAGCGTCATCGTTCACAGACTTGTTTATGAAGGGGCCTTTAAAAGAGGGTTATACACAATCAGTATATGTTCCCTTAACCACGAGAGACACCTCTATTCCCGAGTTATCATCACTGCCTACCGCTGAAACTAATCCCCACATCTTATTGGTATTCTCAACGTGGGACACTCTTGCTCGAGCATTCAAACTGGATCAAGATCAATTCGTAGATTGTCAAGGTCCTCAAGAGTTCTTTGATGCTCAGCTACCATGCCCCGTATCCAATTCGGACGTTGCAGATGCAATCCCCATGACACTGACCACTCTTTCAACTGTATTTTAATCCCAAATTACCTTCATGCAATGGATAAGTACGTATATATTAGAGACGCAATCATTATTATTACTGTCACTTTCCTCTACTCGTTGGAAACGACCAAGCTTTTTTTTTCGCATTTTTTTCGCCGTTGAAAAATTTTTCAATGAGAAAAAGAGCTTTGAAAGGAATGAAAAATTAGCTACAGGTTCGCAGTTGCACTTGTTAGTAGAGTAGCATAGAAGGAAAAAAAGGAGAACAAGGCACATATTTGCAATCTTCACACAATAGCCTGCCAAGCCCGAGATCTACGAAAATGTCCCGTTTGAATTC

Amino Acid Sequence

MSSTRKFKKVEKPLSQTRHYSLCIPTTLVSDCRNLSQITHKVYQVAKFASLFNVSEVVILEDNSQVDATKKKISTAKLILALLQYFVTPPYLRNTVFNEKFRPYLTAASKLPRLSTLPFTRYQKQDHGRYREGLTIKMQKPTLARKKIGKVFKQTKYINIGKSKALALQSQLVPINARVTIDTITRKIVSPQEAYGDFTGLDSQYGYYTRIASSFTDLFMKGPLKEGYTQSVYVPLTTRDTSIPELSSLPTAETNPHILLVFSTWDTLARAFKLDQDQFVDCQGPQEFFDAQLPCPVSNSDVADAIPMTLTTLSTVFZ

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU59580
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of the budding yeast YMR310C including 600 bp from its own promoter and 300 bp from its own 3'UTR in the pRS413 centromeric vector.
• Low Temp: No