cDNA Clone BUL2 (YML111W)

• Plasmid: pRS413 ADH Pr 800- yeast BUL2 (YML111W) +300
• Parent Plasmid: pRS413 ADH Pr
• Expressed: yeast BUL2 (YML111W) including 800 bp promoter and 300 bp 3'UTR

Sequence of Insert

GTCGACTGCAGACGAATAAACTTAACACGGTTTGCAGAGTGTGTTGTTGAGTATAAAACAGTCAATTTCTGTGGACCTGGCCGTAGCCTATTGCTGAAAACACACAAAAGAGGAGTTTGACGAGACTTAAAACAAGCCAAGGAAAATACCATGTTAGACTACTCCCTTCATTCGATGGTTTTACGCCGCATTTCTACACAAATGAATTTACATTCGGGCTGATATGCTTGCTACATAAGCGAATTTAAAATTTGAGAAGGTTCATTGTTGCCTTGTGGTTCGAGCTTAAAGATCATAAAGAACCGCGGGGAATTTGTCTAGTTACAAGCTGGAAAGGACTGAGCAAAGGAAGAGCATTCGTTCACAGAGAGGTCCACGATAGAAAAGTGCCAAGAATATTACTTCTTTCACATCAAAAATGTGTGACGGTTCCTTTGCACGTTGAAGCGTTATCATTTTTTTATGTATATACGGCACCGGTTTTTCGCCTTTTCCTGTTCGTATATTTATAGTCGCCCCGCCATTGCTCGCCCTGTTCCTGGTAGTCTAGGCTCTTTGCATTTCTCTTTTGGTGATTTTTATTTTTTATCTGCCGTTACCAATTAAGAAAAATTTCTGAAATTGTCCAACAGATATGCGTAAAGCAAATATTGCAATTATCATTTTGTGCATAACTTACCAATAATTGATTTCTGTTTCTGTAGGATTTCTCGACAAGAATCTCGACTTCTTACCCGTTTTGGCCAAGGACTTTGCAACTGAAGCAGCAGATTTGAGATATATTCTGGGGAACAAAAGAAGTATTAATGACTTTTACATTCTCCACTTCATCAAGGAAAAATGGGAGACCTCCTTTAAAATCAGTTTCTACGGAAGATAACATTCATCTATTGAGGAAGCGGCGTCAACAGCAGCTGTCAAGTAATTCTACAGATAATTCGCTGCATCCAAATAGTGGGCAGACTCCACGTGCAAGTGACAGCCAGGACGATGACATACGCAGCGCTTCAACAACCAATTTAGACCGTTTACGACAAGAACGAGAGGAAAATTCACTGGAAATGGACTGCACACAGTCAAGATTATCTCATAGAGCAAATATGCTAGTGGATGTCCTACCATCTTTTGAGATGTATAATGCCTTGCACAGACACATACCTCAAGGAAACGTTGATCCCGATAGACATGATTTCCCCCCTTCTTATCAAGAAGTTCGCACTCAAAGAATGACTATATTGCCCAGCAATGATAATTCCGTGGAAAGGTCACAATTGACAGCAGTGCCAGGATCCGAAAACGCATGTAATAATGCGACTGCCCATTCTCTTACCAACCTACATCCTCTACAAACACAACATCTTACAATAAACAGTACAAGAAGTGGTGGACAGTCGCTCCATTCTTCATCAGACACCAATATCAGTCAAATACCGTTCGAAGATGATCTAAACGACTCGGATAACATTTTTATAGACAAGTTATACACCTTACCCAAGTTATCCACTCCTATCGAAATTGATATAAGAATAACGAAAACTGCATCAATACCTCATGAGCGTCCTGAGGAACAGTCAATATTGAAGGAATACACCTCGGGTGATATTATTCATGGTTATTGTTTAATTGAAAATCGGTCCTCTCAACCACTGAAATTTGAAATGTTTTATGTCACTTTAGAAGCGTACATATCTGTAATTGACCGTCAGAAAGGGAAAAGAACGTTAAAGAGATTTTTAAGAATGGTCGATTTGAGCGCATCTTGGTCTTATACAAACATAACCCCAAGTACGGGTATTAATATTGTCCCCGGTGAAAGGGATTTTGACGATGCCATCATTGGTCTTTCAAACAGCAGAGAATTGAAGCCGAACACAAAGTATAAGAAATTCTTCATGTTCAAGCTTCCTACACAGTTATTGGACGTGACTTGCAAACAAGAGCAATTCTCACACTGCCTTCTGCCTCCCAGCTTTGGTATCGACAAGTATAAAAATAATTGTAAATACTCTGGCATAAAAGTAAATAGTGTCCTTGGCTGTGGGCATTTAGGTACAAAAGGTTCACCCATTCTGACACTTGACATGGCAGATGATAATTTATCAATAAATTATACAATTGATGCCAAAATTGTCGGTAAAGATAAAAGAACATCTAAGCTGAATATTATGAAAGAAAAAGAATATAACTTGAGAGTTATGCCCTTCCCCTTTGCTGGTGTCACTAATCAACAAAACGAGAAAACGTGTTTGAGACAACTGAAAAATTTGGAAAGCTTGATTGAAGATAGATTTGAAGCTTTGAACAAGATTTTCAAGAAACTGGAATTAAATGAGGCCATTTCGAATGTAGATATTCATGACACAGATATAAGCGGGACTTTGGACGGTAATGAAGATTTGGATTCCGATGAGATATTAAGACGTAAGCTAGATCAACTGCACATCAATAACAGGATTGATGATACCGCCAGCCAATCACCATCCTATGACTCCAAAAATATGGCTCCAAAGGAAAACCTAGTCGAGACTGAGTTACGCTATAAGTTTAAAAATAAGAATAAATCAAATTCAAGTTTATTTTCTCATTTTTTGAGTTCATCAGAAACGGGGTCATCTTCAACTGGTCCACATGTATATAATTCCGGATTGATTGTATTATCAGTTAAAAAACCGCAATCTGCATTACCTTATTGGTCACCATCATTGTTGAGAAAAACCAACAAATTTGAAGCAAAGAGTGAACAAGAAAAGGAAAACTGGCAAAGGTTAATGGGCATGCTTCCAGAAGGAGTGAAAACCCCACTGACCAAGTTAGATGTACATTTGACTTGTATCCAATCTAATAATAGTGCAGGACATAAGCCGCCAGAAATTAGCTCTGTAACGACAGAATTTGTGGTCATCACAGCAAAGTCTGATAATTCGATTCCTATCAAATTCTGCACTGAACTGTTGATGAATGAGAATAGATTAAACAAACTAAAGACAAAATTTTTGACGTATCAGAAAAAAGTTCATGAATATCGTAAAAAATTTGAAGAAAACCATGCGAAGCTAAACGAGCTTTATAATAGAAATAGAGATCATTTCACTCCAAAAGAGCTTTTGTTCACAAATTTCATATCTGATCAAATAAACAATGATATTGACAGTTTAGCTGGATTAAAAGTTAACATTATTGATTTACATGATATCTTCAAAAAGCAAATACATACTTTTGAAGAAGAAAACGAGGATATTATTTCGAAGAAAGGAAGTTCCAATCCTCCTTCCGCGTCATCCAGTAATAATAATTTCTTACAAGCAACATTTAGCAATGGTGCATCCACCGCTACTAAGTTTACTCAGCAAATTGTGCATGAATGGGAAAAAGTTAAGCCATTACAGTATAAGAGGGATGTTACAGTTAATTTGAAGCTCAACCCCAACATTAAAGAAACGTTAGTGCCCAATTTTGAAACGTGTTTGTGTTGTAGATTTTATTGTGTTCGAGTAAACATCAAATTTGATAACCACTTGGGTTCTATGAAAGTTGATATTCCGGTTGATGTGAAAAAGTTACAAATTTAGACCATACCACACCAACAGTAATCTCGCAGTTTTACAAATAATTGATAAAAGCAGGACTATTTTCTAACCATTTAATATACCTATTACTATTAATATCATTAATATTATTATATTATGTACAGTCACTTATTTCTTTTTTTTTTTTTTTTCTTTCTTTCTTTCGTTCTATTTCCCCCTAGTATATACGAATTCTGCATATCACAGCAAAATATATGGCTATCACATGGCACAGGTATTACTAAAGAAGAAGAGAGACAGAAATAGTATAGGCATGTTTTACTAGCTTCGATTTTCTTGACAAAACTCGAG

Amino Acid Sequence

MTFTFSTSSRKNGRPPLKSVSTEDNIHLLRKRRQQQLSSNSTDNSLHPNSGQTPRASDSQDDDIRSASTTNLDRLRQEREENSLEMDCTQSRLSHRANMLVDVLPSFEMYNALHRHIPQGNVDPDRHDFPPSYQEVRTQRMTILPSNDNSVERSQLTAVPGSENACNNATAHSLTNLHPLQTQHLTINSTRSGGQSLHSSSDTNISQIPFEDDLNDSDNIFIDKLYTLPKLSTPIEIDIRITKTASIPHERPEEQSILKEYTSGDIIHGYCLIENRSSQPLKFEMFYVTLEAYISVIDRQKGKRTLKRFLRMVDLSASWSYTNITPSTGINIVPGERDFDDAIIGLSNSRELKPNTKYKKFFMFKLPTQLLDVTCKQEQFSHCLLPPSFGIDKYKNNCKYSGIKVNSVLGCGHLGTKGSPILTLDMADDNLSINYTIDAKIVGKDKRTSKLNIMKEKEYNLRVMPFPFAGVTNQQNEKTCLRQLKNLESLIEDRFEALNKIFKKLELNEAISNVDIHDTDISGTLDGNEDLDSDEILRRKLDQLHINNRIDDTASQSPSYDSKNMAPKENLVETELRYKFKNKNKSNSSLFSHFLSSSETGSSSTGPHVYNSGLIVLSVKKPQSALPYWSPSLLRKTNKFEAKSEQEKENWQRLMGMLPEGVKTPLTKLDVHLTCIQSNNSAGHKPPEISSVTTEFVVITAKSDNSIPIKFCTELLMNENRLNKLKTKFLTYQKKVHEYRKKFEENHAKLNELYNRNRDHFTPKELLFTNFISDQINNDIDSLAGLKVNIIDLHDIFKKQIHTFEEENEDIISKKGSSNPPSASSSNNNFLQATFSNGASTATKFTQQIVHEWEKVKPLQYKRDVTVNLKLNPNIKETLVPNFETCLCCRFYCVRVNIKFDNHLGSMKVDIPVDVKKLQI*

• Vector Type: Yeast
• DU Number: DU59788
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of the budding yeast BUL2 (YML111W) including 1000 bp from its own promoter and 300 bp from its own 3'UTR in the pRS413 centromeric vector.
• Low Temp: No