Synonyms
HRCA1; pVHL; RCA1; VHL1
Plasmid
pcDNA5D FRT/TO FLAG VHL 5Gly aASC
Parent Plasmid
pcDNA5D FRT/TO FLAG
Expressed
FLAG VHL 5Gly aASC
Genbank
VHL: NM_000551.3 (one silent change) aASC: Anti-ASC nanobody for use in degron work. Codon optimised.
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
ggatccGCCATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTCGAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCGGAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGTCGGCCGCGGCCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGAGATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGGCTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATGGTGGAGGCGGAGGTGAATTCGCCATGGCTCAGGTCCAGCTGCAAGAGTCTGGCGGAGGACTTGTTCAGCCTGGCGGAAGCCTGAAGCTGTCTTGTGCCGCCAGCGGCTTCACCTTCAGCAGATACGCCATGAGCTGGTACAGACAGGCCCCTGGCAAAGAAAGGGAAAGCGTGGCCAGAATCAGCAGCGGCGGAGGCACAATCTACTACGCCGATAGCGTGAAGGGCAGATTCACCATCTCCAGAGAGGACGCCAAGAACACCGTGTACCTGCAGATGAACTCCCTGAAGCCTGAGGACACCGCCGTGTACTACTGTTACGTCGGCGGCTTTTGGGGCCAGGGCACACAAGTGACAGTGTCTAGCGGAGGCTGAgcggccgc
Amino Acid Sequence
View Sequence
MDYKDDDDKGSAMPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVW
LNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDL
EDHPNVQKDLERLTQERIAHQRMGDGGGGGEFAMAQVQLQESGGGLVQPGGSLKLSCAASGFTFSRYAMSWYRQAPGKERESVARISSGGGTIYYADSVK
GRFTISREDAKNTVYLQMNSLKPEDTAVYYCYVGGFWGQGTQVTVSSGG*
LNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDL
EDHPNVQKDLERLTQERIAHQRMGDGGGGGEFAMAQVQLQESGGGLVQPGGSLKLSCAASGFTFSRYAMSWYRQAPGKERESVARISSGGGTIYYADSVK
GRFTISREDAKNTVYLQMNSLKPEDTAVYYCYVGGFWGQGTQVTVSSGG*
Vector Type
Mammalian
Antibiotic
Amp
Full Snapgene Map
pcDNA5D FRT/TO FLAG VHL 5Gly aASC147.37 KBSnapgene files can be opened using software available here: www.snapgene.com
DU Number
DU63121
Unit Source
Location
PPU
MTA
Low Temp
No
Price
£125.00
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