cDNA Clone YPL191c

• Plasmid: pYes-GAL-6His-YPL191c-C28A
• Parent Plasmid: pYES2
• Expressed: GAL-6His-YPL191c-C28A
• Genbank: NP_015133.1
• Tag: 6His
• Tag: GAL4

Sequence of Insert

GGATCCATGCATCACCATCACCATCACATGGATTTAAGTTTCACGACTAAAAGTGTCAAAATTAATGGTCAAAACCATCGAATATTACTGCAAAACGAAAACGGACCTGCTGCCTTGTTAGCACTTGCAAATATTCTAATACTTTCACCAGATCATACACGTTTTTCCAATGAGTTAATAAGGTTGGTAAATAAGGGAAGTCAAATATCTTTAAAAGAACTTATTGAAGTCTTAGCAGATATCGCTTTACAAGTTACAGATAAACCTAGTACTGATATAAGTGAGTTACTAAGTTTGTTACCTAGATTACATGAAGGTTTAAATATTAACCCTGAGTTTAATGGGTCTTTTGAAAATACTAAAGAGATGTCAATATTTCGATTGTTTAACGTTGATGTTGTCCATGGCTGGGTTATCAACAGTTTCATAAATGAAAACATTGATGAGAAACTATCTCATTATTCTTATGAATCTGCGCAGAGAATCTTAACACAAGCAGCTGATATAAATTGTGGAATTTCCCAAGATGAAAACTCGGACGAAGTTTTACGTGATGCCATGCATTTGGGTCTGTTTTTGAACGAATCACCGACTCAGTTGACCGCCTTTGGACTCCTACGATTGAGAGAAAAGCTGCTACACAACAAATTCTCCATTTTATTCAGAAATGACCATTTTTCTACATTGTTCAAATACGAAGATCGATTATACACATTGGTTACGGACTTTGGTTATAAAAATTGTAAAGATATTGTATGGCAATCCCTAGATTCGGTGGACGGCTCCTGCGATGCTTTTTTTGCCGGTAACTTTAGTGCAGCAGAGGTAAATGGACAGCAATTGTCAACTGACATTGAACGTGATTTTGGAACTGGAAATTTGCTACTTGAAGAAATTCAGCAAATTGAAAACGATAAAGAACTAGCCAAACAGTTACAGGAACAAGAACAAGAACGTGTTACGAAATTTGAAGCAAAAAGGAAAATCCATTCTCACAAAAAAAACTCAGAAATACACGCACCAGTCAAAAAAGACAAGTTTAAGCGAAGAAGCAGCCTTCTCAATGCAAAAGCTTCTGAAAAAGAGAAAAGTGAGTGCGTTGTTATGTAAGCGGCCGC

Amino Acid Sequence

MHHHHHHMDLSFTTKSVKINGQNHRILLQNENGPAALLALANILILSPDHTRFSNELIRLVNKGSQISLKELIEVLADIALQVTDKPSTDISELLSLLPRLHEGLNINPEFNGSFENTKEMSIFRLFNVDVVHGWVINSFINENIDEKLSHYSYESAQRILTQAADINCGISQDENSDEVLRDAMHLGLFLNESPTQLTAFGLLRLREKLLHNKFSILFRNDHFSTLFKYEDRLYTLVTDFGYKNCKDIVWQSLDSVDGSCDAFFAGNFSAAEVNGQQLSTDIERDFGTGNLLLEEIQQIENDKELAKQLQEQEQERVTKFEAKRKIHSHKKNSEIHAPVKKDKFKRRSSLLNAKASEKEKSECVVM*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU55929
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please, can you clone the mammalian gene YPL191c-C28Ain the yeast plasmid pYes, to have the final plasmid pYes_Gal promoter - 6His - YPL191c-C28A. Thank you
• Low Temp: No