cDNA Clone alpha-Tubulin

• Synonyms: TUBA1A
• Plasmid: pSc-B GFP-TUBA1A
• Parent Plasmid: pSC-B
• Genbank: 7846
• Tag: GFP
• Species: Human

Sequence of Insert

GCTAGCGCCACCATGCATGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGT
GGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCA
GCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTG
AAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGT
GACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACA
TGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAG
GAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGA
GGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCA
TCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAAC
TACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCAT
CAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGC
TCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTG
CTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCC
CAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCG
GGATCACTCTCGGCATGGACGAGCTGTACAAGTCCGGACTCGGATCCATG
CGTGAGTGCATCTCCATCCACGTTGGCCAGGCTGGTGTCCAGATTGGCAA
TGCCTGCTGGGAGCTCTACTGCCTGGAACACGGCATCCAGCCCGATGGCC
AGATGCCAAGTGACAAGACCATTGGGGGAGGAGATGACTCCTTCAACACC
TTCTTCAGTGAGACGGGCGCTGGCAAGCACGTGCCCCGGGCTGTGTTTGT
AGACTTGGAACCCACAGTCATTGATGAAGTTCGCACTGGCACCTACCGCC
AGCTCTTCCACCCTGAGCAGCTCATCACAGGCAAGGAAGATGCTGCCAAT
AACTATGCCCGAGGGCACTACACCATTGGCAAGGAGATCATTGACCTTGT
GTTGGACCGAATTCGCAAGCTGGCTGACCAGTGCACCGGTCTTCAGGGCT
TCTTGGTTTTCCACAGCTTTGGTGGGGGAACTGGTTCTGGGTTCACCTCC
CTGCTCATGGAACGTCTCTCAGTTGATTATGGCAAGAAATCCAAGCTGGA
GTTCTCCATTTACCCGGCACCCCAGGTTTCCACAGCTGTAGTTGAGCCCT
ACAACTCCATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCC
TTCATGGTAGACAATGAGGCCATCTATGACATCTGTCGTAGAAACCTCGA
TATCGAGCGCCCAACCTACACTAACCTTAACCGCCTTATTGGTCAGATTG
TGTCCTCCATCACTGCTTCCCTGAGATTTGATGGAGCCCTGAATGTTGAC
CTGACAGAATTCCAGACCAACCTGGTGCCCTACCCCCGCATCCACTTCCC
TCTGGCCACATATGCCCCTGTCATCTCTGCTGAGAAAGCCTACCATGAAC
AGCTTTCTGTAGCAGAGATCACCAATGCTTGCTTTGAGCCAGCCAACCAG
ATGGTGAAATGTGACCCTCGCCATGGTAAATACATGGCTTGCTGCCTGTT
GTACCGTGGTGACGTGGTTCCCAAAGATGTCAATGCTGCCATTGCCACCA
TCAAAACCAAGCGCACCATCCAGTTTGTGGATTGGTGCCCCACTGGCTTC
AAGGTTGGCATCAACTACCAGCCTCCCACTGTGGTGCCTGGTGGAGACCT
GGCCAAGGTACAGAGAGCTGTGTGCATGCTGAGCAACACCACAGCCATTG
CTGAGGCCTGGGCTCGCCTGGACCACAAGTTTGACCTGATGTATGCCAAG
CGTGCCTTTGTTCACTGGTACGTGGGTGAGGGGATGGAGGAAGGCGAGTT
TTCAGAGGCCCGTGAAGATATGGCTGCCCTTGAGAAGGATTATGAGGAGG
TTGGTGTGGATTCTGTTGAAGGAGAGGGTGAGGAAGAAGGAGAGGAATAC
TAActcgag

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMRECI
SIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSE
TGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYAR
GHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLME
RLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVD
NEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEF
QTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKC
DPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGI
NYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFV
HWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU28843
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone contains the following silent change compared to NCBI ref shown:; D47 (GAT > GAC), G57 (GGG > GGC), H61 (CAT > CAC), A65 (GCA > GCT), Q91 (CAA > CAG), L92 (CTT > CTC), K96 (AAA > AAG), L117 (CTC > CTT), A126 (GCC > GCT), T130 (ACG > ACC), L132 (CTC > CTT), S151 (TCG > TCC), K164 (AAG > AAA), S170 (TCT > TCC), A174 (GCG > GCA), I219 (ATT > ATC), R221 (CGT > CGC), Y224 (TAT > TAC), L227 (CTG > CTT), N228 (AAT > AAC), R229 (AGG > CGC), L230 (TTA > CTT), I231 (ATA > ATT), Q233 (CAA > CAG), Y262 (TAT > TAC), K336 (AAG > AAA), R339 (CGT > CGC), K401 (AAA > AAG), V409 (GTT > GTG), G410 (GGG > GGT), G416 (GGT > GGC), E423 (GAG > GAA), D424 (GAC > GAT),
• Low Temp: No