cDNA Clone WNK4

• Synonyms: PHA2B; PRKWNK4
• Plasmid: pEBG2T Flag WNK4 D1131-L1167 L1155Q K1158A
• Parent Plasmid: pEBG2T
• Expressed: GST Thr Flag WNK4 D1131-L1167 L1155Q K1158A
• Genbank: NM_032387.4 GI:260166636
• Tag: GST
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGGATGAGGAGTTCTGGGCTGAGCTGCAGAGTCTTCGGCAGAAGCACTTGTCAGAGGTGGAAACACTACAGACACAACAGAAAGCAGAAATTGAAGATTTGTACAGCCGGCTGTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSATMDYKDDDDKDEEFWAELQSLRQKHLSEVETLQTQQKAEIEDLYSRL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU53756
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.
• Low Temp: No