cDNA Clone
CDC53/Mcm7

Plasmid
pKL2112 plus NotI/SacI SpMcm7 insert from pKL2097 (intermediate)
Parent Plasmid
pRS306
Species
Yeast
Sequence of Insert
GCGGCCGCtataaaacaATGGCTTTGCCAACTATTGACTTGAAGATTCCAGAATACGAAGAATGTCAACACAAGATTACTGATTTCTTGTCCCACTTCAA
GCAAGAACAAGTTCAAGATGGTCAACAAAACCAAGATATTTCTATGTCCGATGCTGGTGATGAACCATTCTTGAAGTCCAAGTACATGGATATTTTGCAA
AAGATCTCTAACAGAGAATCCAACGTCATCAACGTTGACTTGAACGATTTGTACGAATTCGATCCATCCGATACTCAATTGTTGCACAACATTGAATCTA
ACGCTAAGAGATTCGTTGAATTGTTCTCCCAATGTGCTGACGCTTTGATGCCACCACCAACTGTTGAAATCAACTACAGAAACGAAGTCTTGGATGTCAT
CATGCAACAAAGAGTTCAAAGAAACGAAAACATTGACCCAGAACACAAGGGTTTCCCACCAGAATTGACTAGAGGTTACGACTTGTACTTCAGACCAGTC
ACTAGAAACAAGAAGCCATTCTCCGTCAGAGATTTGAGAGGTGAAAACTTGGGTTCCTTGTTGACTGTCAGAGGTATCGTCACTAGAACTTCCGACGTCA
AGCCATCTTTGACTGTTAACGCTTACACTTGTGATAGATGTGGTTACGAAGTTTTCCAAGAAATTAGACAAAAGACTTTCTTGCCAATGTCCGAATGTCC
ATCCGATGAATGTAAGAAGAACGACGCTAAGGGTCAATTGTTCATGTCTACTAGAGCTTCTAAGTTCTTGCCATTCCAAGAAGTTAAGATTCAAGAATTG
ACCAACCAAGTCCCAATTGGTCACATTCCAAGATCTTTGACCGTTCACTTGTACGGTGCTATTACTAGATCCGTCAACCCAGGTGATATTGTCGACATCT
CTGGTATCTTCTTGCCAACCCCATACACCGGTTTCAGAGCTATGAGAGCTGGTTTGTTGACTGACACTTACTTGGAATGTCACTACGTCTCCCAAATCAT
CAAGAACTACACTAACATCGAAAAGACTCCACAATCTGAAGCTGCTATCGCTGAATTGAACCAAGGTGGTAACGTTTACGAAAAGTTGGCTAAGTCTATT
GCTCCAGAAATCTACGGTCACGAAGACGTTAAGAAGGCTTTGTTGTTGTTGTTGGTCGGTGGTGTCACCAAGGAATTGGGTGACGGTATGAGAATCAGAG
GTGACATTAACATCTGTTTGACCGGTGACCCAGGTGTCGCTAAGTCTCAATTGTTGAAGTACATTTCTAAGGTCGCTCCAAGAGGTGTTTACACTACTGG
TAGAGGTTCTTCCGGTGTCGGTTTGACCGCTGCTGTCATGAGAGACCCAGTTACCGACGAAATGGTTTTGGAAGGTGGTGCTTTGGTTTTGGCTGATAAC
GGTATTTGTTGTATCGATGAATTCGACAAGATGGATGAATCTGATAGAACTGCTATTCACGAAGTTATGGAACAACAAACCATTTCCATTTCTAAGGCTG
GTATCACTACCACTTTGAACGCTAGAACCTCTATCTTGGCTGCTGCTAACCCATTGTACGGTAGATACAACCCAAAGGTTGCTCCAATCCACAACATTAA
CTTGCCAGCTGCTTTGTTGTCTAGATTCGATATTTTGTTCTTGATCTTGGACACCCCATCTAGAGAAACTGACGAACACTTGGCTCAACACGTTACCTAC
GTTCACATGCACAACGAACAACCAAAGATGGACTTCGAACCATTGGATCCAAACATGATCAGACACTACATTTCTTCTGCTAGACAATACAGACCAGTTG
TTCCAAAGGATGTCTGTGATTACGTTACCGGTGCTTACGTCCAATTGAGACAAAACCAAAAGAGAGACGAAGCTAACGAAAGACAATTCGCTCACACTAC
TCCAAGAACTTTGTTGGCTATCTTGAGAATGGGTCAAGCTTTGGCTAGATTGAGATTCTCCAACAGAGTCGAAATCGGTGACGTTGATGAAGCTTTGAGA
TTGATGTCTGTTTCTAAGTCCTCCTTGTACGATGACTTGGACCCATCCTCTCACGATACTACCATCACTTCTAAGATTTACAAGATCATTAGAGATATGT
TGAACTCCATTCCAGACGTCGAAGGTAACGAAAGATCCTTGACTTTGAGAGCTATTAGAGAAAGAGTTTTGGCTAAGGGTTTCACTGAAGACCACTTGAT
CAACACCATCCAAGAATACACCGACTTGGGTGTCTTGTTGACCACCAACAACGGTCAAACTATTATGTTCTTGGACCCAGACTTGCACATGGAAAACtaa
attgaattgaattgaaatcgatagatcaatttttttcttttctctttccccatcctttacgctaaaataatagtttattttattttttgaatatttttta
tttatatacgtatatatagactattatttatcttttaatgattattaagatttttattaaaaaaaaattcgctcctcttttaatgcctttatgcGAGCTC
Vector Type
Yeast
Antibiotic
Amp
DU Number
DU53725
Unit Source
Protein Phosphorylation and Ubiquitylation Unit
Location
PPU
MTA
Dundee
Notes
2.5kb NotI/SacI insert (SpMcm7) from pKL2097 into NotI/SacI of pKL2112
Schizosaccharomyces pombe
Low Temp
No
Price
£125.00
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